Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients

Prostate cancer is a heterogeneous disease that remains dormant for long periods or acts aggressively with poor clinical outcomes. Identifying aggressive prostate tumor behavior using current glandular-focused histopathological criteria is challenging. Recent evidence has implicated the stroma in mo...

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Autores principales:	Ina P. Pavlova, Sujit S. Nair, Dara Lundon, Stanislaw Sobotka, Reza Roshandel, Patrick-Julien Treacy, Parita Ratnani, Rachel Brody, Jonathan I. Epstein, Gustavo E. Ayala, Natasha Kyprianou, Ashutosh K. Tewari
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	optical microscopy prostate cancer reactive stroma collagen signatures prognosis Medicine R
Acceso en línea:	https://doaj.org/article/d8316fc960fc40c6ac773fb15e79a94d
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spelling	oai:doaj.org-article:d8316fc960fc40c6ac773fb15e79a94d2021-11-25T18:06:48ZMultiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients10.3390/jpm111110612075-4426https://doaj.org/article/d8316fc960fc40c6ac773fb15e79a94d2021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4426/11/11/1061https://doaj.org/toc/2075-4426Prostate cancer is a heterogeneous disease that remains dormant for long periods or acts aggressively with poor clinical outcomes. Identifying aggressive prostate tumor behavior using current glandular-focused histopathological criteria is challenging. Recent evidence has implicated the stroma in modulating prostate tumor behavior and in predicting post-surgical outcomes. However, the emergence of stromal signatures has been limited, due in part to the lack of adoption of imaging modalities for stromal-specific profiling. Herein, label-free multiphoton microscopy (MPM), with its ability to image tissue with stromal-specific contrast, is used to identify prostate stromal features associated with aggressive tumor behavior and clinical outcome. MPM was performed on unstained prostatectomy specimens from 59 patients and on biopsy specimens from 17 patients with known post-surgery recurrence status. MPM-identified collagen content, organization, and morphological tumor signatures were extracted for each patient and screened for association with recurrent disease. Compared to tumors from patients whose disease did not recur, tumors from patients with recurrent disease exhibited higher MPM-identified collagen amount and collagen fiber intensity signal and width. Our study shows an association between MPM-identified stromal collagen features of prostate tumors and post-surgical disease recurrence, suggesting their potential for prostate cancer risk assessment.Ina P. PavlovaSujit S. NairDara LundonStanislaw SobotkaReza RoshandelPatrick-Julien TreacyParita RatnaniRachel BrodyJonathan I. EpsteinGustavo E. AyalaNatasha KyprianouAshutosh K. TewariMDPI AGarticleoptical microscopyprostate cancerreactive stromacollagen signaturesprognosisMedicineRENJournal of Personalized Medicine, Vol 11, Iss 1061, p 1061 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	optical microscopy prostate cancer reactive stroma collagen signatures prognosis Medicine R
spellingShingle	optical microscopy prostate cancer reactive stroma collagen signatures prognosis Medicine R Ina P. Pavlova Sujit S. Nair Dara Lundon Stanislaw Sobotka Reza Roshandel Patrick-Julien Treacy Parita Ratnani Rachel Brody Jonathan I. Epstein Gustavo E. Ayala Natasha Kyprianou Ashutosh K. Tewari Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients
description	Prostate cancer is a heterogeneous disease that remains dormant for long periods or acts aggressively with poor clinical outcomes. Identifying aggressive prostate tumor behavior using current glandular-focused histopathological criteria is challenging. Recent evidence has implicated the stroma in modulating prostate tumor behavior and in predicting post-surgical outcomes. However, the emergence of stromal signatures has been limited, due in part to the lack of adoption of imaging modalities for stromal-specific profiling. Herein, label-free multiphoton microscopy (MPM), with its ability to image tissue with stromal-specific contrast, is used to identify prostate stromal features associated with aggressive tumor behavior and clinical outcome. MPM was performed on unstained prostatectomy specimens from 59 patients and on biopsy specimens from 17 patients with known post-surgery recurrence status. MPM-identified collagen content, organization, and morphological tumor signatures were extracted for each patient and screened for association with recurrent disease. Compared to tumors from patients whose disease did not recur, tumors from patients with recurrent disease exhibited higher MPM-identified collagen amount and collagen fiber intensity signal and width. Our study shows an association between MPM-identified stromal collagen features of prostate tumors and post-surgical disease recurrence, suggesting their potential for prostate cancer risk assessment.
format	article
author	Ina P. Pavlova Sujit S. Nair Dara Lundon Stanislaw Sobotka Reza Roshandel Patrick-Julien Treacy Parita Ratnani Rachel Brody Jonathan I. Epstein Gustavo E. Ayala Natasha Kyprianou Ashutosh K. Tewari
author_facet	Ina P. Pavlova Sujit S. Nair Dara Lundon Stanislaw Sobotka Reza Roshandel Patrick-Julien Treacy Parita Ratnani Rachel Brody Jonathan I. Epstein Gustavo E. Ayala Natasha Kyprianou Ashutosh K. Tewari
author_sort	Ina P. Pavlova
title	Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients
title_short	Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients
title_full	Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients
title_fullStr	Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients
title_full_unstemmed	Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients
title_sort	multiphoton microscopy for identifying collagen signatures associated with biochemical recurrence in prostate cancer patients
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d8316fc960fc40c6ac773fb15e79a94d
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Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients

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