Transcriptome analysis discloses dysregulated genes in normal appearing tumor-adjacent thyroid tissues from patients with papillary thyroid carcinoma

Abstract Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. The molecular characteristics of histologically normal appearing tissue adjacent to the tumor (NAT) from PTC patients are not well characterized. The aim of this study was to characterize the global gene expression...

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Autores principales:	Huiling He, Sandya Liyanarachchi, Wei Li, Daniel F. Comiskey, Pearlly Yan, Ralf Bundschuh, Altan M. Turkoglu, Pamela Brock, Matthew D. Ringel, Albert de la Chapelle
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d833978c405a45319b21b5be60f8ba9c
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spelling	oai:doaj.org-article:d833978c405a45319b21b5be60f8ba9c2021-12-02T15:39:51ZTranscriptome analysis discloses dysregulated genes in normal appearing tumor-adjacent thyroid tissues from patients with papillary thyroid carcinoma10.1038/s41598-021-93526-92045-2322https://doaj.org/article/d833978c405a45319b21b5be60f8ba9c2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93526-9https://doaj.org/toc/2045-2322Abstract Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. The molecular characteristics of histologically normal appearing tissue adjacent to the tumor (NAT) from PTC patients are not well characterized. The aim of this study was to characterize the global gene expression profile of NAT and compare it with those of normal and tumor thyroid tissues. We performed total RNA sequencing with fresh frozen thyroid tissues from a cohort of three categories of samples including NAT, normal thyroid (N), and PTC tumor (T). Transcriptome analysis shows that NAT presents a unique gene expression profile, which was not associated with sex or the presence of lymphocytic thyroiditis. Among the differentially expressed genes (DEGs) of NAT vs N, 256 coding genes and 5 noncoding genes have been reported as cancer genes involved in cell proliferation, apoptosis, and/or tumorigenesis. Bioinformatics analysis with Ingenuity Pathway Analysis software revealed that “Cancer, Organismal Injury and Abnormalities, Cellular Response to Therapeutics, and Cellular Movement” were major dysregulated pathways in the NAT tissues. This study provides improved insight into the complexity of gene expression changes in the thyroid glands of patients with PTC.Huiling HeSandya LiyanarachchiWei LiDaniel F. ComiskeyPearlly YanRalf BundschuhAltan M. TurkogluPamela BrockMatthew D. RingelAlbert de la ChapelleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Huiling He Sandya Liyanarachchi Wei Li Daniel F. Comiskey Pearlly Yan Ralf Bundschuh Altan M. Turkoglu Pamela Brock Matthew D. Ringel Albert de la Chapelle Transcriptome analysis discloses dysregulated genes in normal appearing tumor-adjacent thyroid tissues from patients with papillary thyroid carcinoma
description	Abstract Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. The molecular characteristics of histologically normal appearing tissue adjacent to the tumor (NAT) from PTC patients are not well characterized. The aim of this study was to characterize the global gene expression profile of NAT and compare it with those of normal and tumor thyroid tissues. We performed total RNA sequencing with fresh frozen thyroid tissues from a cohort of three categories of samples including NAT, normal thyroid (N), and PTC tumor (T). Transcriptome analysis shows that NAT presents a unique gene expression profile, which was not associated with sex or the presence of lymphocytic thyroiditis. Among the differentially expressed genes (DEGs) of NAT vs N, 256 coding genes and 5 noncoding genes have been reported as cancer genes involved in cell proliferation, apoptosis, and/or tumorigenesis. Bioinformatics analysis with Ingenuity Pathway Analysis software revealed that “Cancer, Organismal Injury and Abnormalities, Cellular Response to Therapeutics, and Cellular Movement” were major dysregulated pathways in the NAT tissues. This study provides improved insight into the complexity of gene expression changes in the thyroid glands of patients with PTC.
format	article
author	Huiling He Sandya Liyanarachchi Wei Li Daniel F. Comiskey Pearlly Yan Ralf Bundschuh Altan M. Turkoglu Pamela Brock Matthew D. Ringel Albert de la Chapelle
author_facet	Huiling He Sandya Liyanarachchi Wei Li Daniel F. Comiskey Pearlly Yan Ralf Bundschuh Altan M. Turkoglu Pamela Brock Matthew D. Ringel Albert de la Chapelle
author_sort	Huiling He
title	Transcriptome analysis discloses dysregulated genes in normal appearing tumor-adjacent thyroid tissues from patients with papillary thyroid carcinoma
title_short	Transcriptome analysis discloses dysregulated genes in normal appearing tumor-adjacent thyroid tissues from patients with papillary thyroid carcinoma
title_full	Transcriptome analysis discloses dysregulated genes in normal appearing tumor-adjacent thyroid tissues from patients with papillary thyroid carcinoma
title_fullStr	Transcriptome analysis discloses dysregulated genes in normal appearing tumor-adjacent thyroid tissues from patients with papillary thyroid carcinoma
title_full_unstemmed	Transcriptome analysis discloses dysregulated genes in normal appearing tumor-adjacent thyroid tissues from patients with papillary thyroid carcinoma
title_sort	transcriptome analysis discloses dysregulated genes in normal appearing tumor-adjacent thyroid tissues from patients with papillary thyroid carcinoma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d833978c405a45319b21b5be60f8ba9c
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Transcriptome analysis discloses dysregulated genes in normal appearing tumor-adjacent thyroid tissues from patients with papillary thyroid carcinoma

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