Enhanced photocytotoxicity of curcumin delivered by solid lipid nanoparticles

Shan Jiang,1 Rongrong Zhu,1 Xiaolie He,1 Jiao Wang,1 Mei Wang,1 Yechang Qian,2 Shilong Wang1 1Tenth People’s Hospital, School of Life Science and Technology, Tongji University, 2Department of Respiratory Disease, Baoshan District Hospital of Integrated Traditional Chinese and Western Medi...

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Autores principales:	Jiang S, Zhu R, He X, Wang J, Wang M, Qian Y, Wang S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Photodynamic therapy Curcumin Solid lipid nanoparticles Drug delivery Reactive oxygen species Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d840144ac0544d05ac0d79ce8d08b9a0
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spelling	oai:doaj.org-article:d840144ac0544d05ac0d79ce8d08b9a02021-12-02T05:40:37ZEnhanced photocytotoxicity of curcumin delivered by solid lipid nanoparticles1178-2013https://doaj.org/article/d840144ac0544d05ac0d79ce8d08b9a02016-12-01T00:00:00Zhttps://www.dovepress.com/enhanced-photocytotoxicity-of-curcumin-delivered-by-solid-lipid-nanopa-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Shan Jiang,1 Rongrong Zhu,1 Xiaolie He,1 Jiao Wang,1 Mei Wang,1 Yechang Qian,2 Shilong Wang1 1Tenth People’s Hospital, School of Life Science and Technology, Tongji University, 2Department of Respiratory Disease, Baoshan District Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, People’s Republic of China Abstract: Curcumin (Cur) is a promising photosensitizer that could be used in photodynamic therapy. However, its poor solubility and hydrolytic instability limit its clinical use. The aim of the present study was to encapsulate Cur into solid lipid nanoparticles (SLNs) in order to improve its therapeutic activity. The Cur-loaded SLNs (Cur-SLNs) were prepared using an emulsification and low-temperature solidification method. The functions of Cur and Cur-SLNs were studied on the non-small cell lung cancer A549 cells for photodynamic therapy. The results revealed that Cur-SLNs induced ~2.27-fold toxicity higher than free Cur at a low concentration of 15 µM under light excitation, stocking more cell cycle at G2/M phase. Cur-SLNs could act as an efficient drug delivery system to increase the intracellular concentration of Cur and its accumulation in mitochondria; meanwhile, the hydrolytic stability of free Cur could be improved. Furthermore, Cur-SLNs exposed to 430 nm light could produce more reactive oxygen species to induce the disruption of mitochondrial membrane potential. Western blot analysis revealed that Cur-SLNs increased the expression of caspase-3, caspase-9 proteins and promoted the ratio of Bax/Bcl-2. Overall, the results from these studies demonstrated that the SLNs could enhance the phototoxic effects of Cur. Keywords: photodynamic therapy, curcumin, solid lipid nanoparticles, drug delivery, reactive oxygen speciesJiang SZhu RHe XWang JWang MQian YWang SDove Medical PressarticlePhotodynamic therapyCurcuminSolid lipid nanoparticlesDrug deliveryReactive oxygen speciesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 167-178 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Photodynamic therapy Curcumin Solid lipid nanoparticles Drug delivery Reactive oxygen species Medicine (General) R5-920
spellingShingle	Photodynamic therapy Curcumin Solid lipid nanoparticles Drug delivery Reactive oxygen species Medicine (General) R5-920 Jiang S Zhu R He X Wang J Wang M Qian Y Wang S Enhanced photocytotoxicity of curcumin delivered by solid lipid nanoparticles
description	Shan Jiang,1 Rongrong Zhu,1 Xiaolie He,1 Jiao Wang,1 Mei Wang,1 Yechang Qian,2 Shilong Wang1 1Tenth People’s Hospital, School of Life Science and Technology, Tongji University, 2Department of Respiratory Disease, Baoshan District Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, People’s Republic of China Abstract: Curcumin (Cur) is a promising photosensitizer that could be used in photodynamic therapy. However, its poor solubility and hydrolytic instability limit its clinical use. The aim of the present study was to encapsulate Cur into solid lipid nanoparticles (SLNs) in order to improve its therapeutic activity. The Cur-loaded SLNs (Cur-SLNs) were prepared using an emulsification and low-temperature solidification method. The functions of Cur and Cur-SLNs were studied on the non-small cell lung cancer A549 cells for photodynamic therapy. The results revealed that Cur-SLNs induced ~2.27-fold toxicity higher than free Cur at a low concentration of 15 µM under light excitation, stocking more cell cycle at G2/M phase. Cur-SLNs could act as an efficient drug delivery system to increase the intracellular concentration of Cur and its accumulation in mitochondria; meanwhile, the hydrolytic stability of free Cur could be improved. Furthermore, Cur-SLNs exposed to 430 nm light could produce more reactive oxygen species to induce the disruption of mitochondrial membrane potential. Western blot analysis revealed that Cur-SLNs increased the expression of caspase-3, caspase-9 proteins and promoted the ratio of Bax/Bcl-2. Overall, the results from these studies demonstrated that the SLNs could enhance the phototoxic effects of Cur. Keywords: photodynamic therapy, curcumin, solid lipid nanoparticles, drug delivery, reactive oxygen species
format	article
author	Jiang S Zhu R He X Wang J Wang M Qian Y Wang S
author_facet	Jiang S Zhu R He X Wang J Wang M Qian Y Wang S
author_sort	Jiang S
title	Enhanced photocytotoxicity of curcumin delivered by solid lipid nanoparticles
title_short	Enhanced photocytotoxicity of curcumin delivered by solid lipid nanoparticles
title_full	Enhanced photocytotoxicity of curcumin delivered by solid lipid nanoparticles
title_fullStr	Enhanced photocytotoxicity of curcumin delivered by solid lipid nanoparticles
title_full_unstemmed	Enhanced photocytotoxicity of curcumin delivered by solid lipid nanoparticles
title_sort	enhanced photocytotoxicity of curcumin delivered by solid lipid nanoparticles
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/d840144ac0544d05ac0d79ce8d08b9a0
work_keys_str_mv	AT jiangs enhancedphotocytotoxicityofcurcumindeliveredbysolidlipidnanoparticles AT zhur enhancedphotocytotoxicityofcurcumindeliveredbysolidlipidnanoparticles AT hex enhancedphotocytotoxicityofcurcumindeliveredbysolidlipidnanoparticles AT wangj enhancedphotocytotoxicityofcurcumindeliveredbysolidlipidnanoparticles AT wangm enhancedphotocytotoxicityofcurcumindeliveredbysolidlipidnanoparticles AT qiany enhancedphotocytotoxicityofcurcumindeliveredbysolidlipidnanoparticles AT wangs enhancedphotocytotoxicityofcurcumindeliveredbysolidlipidnanoparticles
_version_	1718400323723198464

Enhanced photocytotoxicity of curcumin delivered by solid lipid nanoparticles

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