NanoMIP-Based Solid Phase Extraction of Fluoroquinolones from Human Urine: A Proof-of-Concept Study

NanoMIPs that are prepared by solid phase synthesis have proven to be very versatile, but to date only limited attention has been paid to their use in solid phase extraction. Thus, since nanoMIPs show close similarities, in terms of binding behavior, to antibodies, it seems relevant to verify if it...

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Autores principales:	Matteo Chiarello, Laura Anfossi, Simone Cavalera, Fabio Di Nardo, Thea Serra, Claudio Baggiani
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	molecularly imprinted polymer nanoMIP solid phase synthesis MISPE solid phase extraction fluoroquinolone Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d840eccdbd354d498c29f5c7347042fd
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spelling	oai:doaj.org-article:d840eccdbd354d498c29f5c7347042fd2021-11-25T18:59:30ZNanoMIP-Based Solid Phase Extraction of Fluoroquinolones from Human Urine: A Proof-of-Concept Study10.3390/separations81102262297-8739https://doaj.org/article/d840eccdbd354d498c29f5c7347042fd2021-11-01T00:00:00Zhttps://www.mdpi.com/2297-8739/8/11/226https://doaj.org/toc/2297-8739NanoMIPs that are prepared by solid phase synthesis have proven to be very versatile, but to date only limited attention has been paid to their use in solid phase extraction. Thus, since nanoMIPs show close similarities, in terms of binding behavior, to antibodies, it seems relevant to verify if it is possible to use them as mimics of the natural antibodies that are used in immunoextraction methods. As a proof-of-concept, we considered prepared nanoMIPs against fluoroquinolone ciprofloxacin. Several nanoMIPs were prepared in water with polymerization mixtures of different compositions. The polymer with the highest affinity towards ciprofloxacin was then grafted onto a solid support and used to set up a solid phase extraction–HPLC method with fluorescence detection, for the determination of fluoroquinolones in human urine. The method resulted in successful selection for the fluoroquinolone antibiotics, such that the nanoMIPs were suitable for direct extraction of the antibiotics from the urine samples at the µg mL<sup>−1</sup> level. They required no preliminary treatment, except for a 1 + 9 (<i>v</i>/<i>v</i>) dilution with a buffer of pH 4.5 and they had good analyte recovery rates; up to 85% with precision in the range of 3 to 4.5%, without interference from the matrix. These experimental results demonstrate, for the first time, the feasibility of the use of nanoMIPs to develop solid phase extraction methods.Matteo ChiarelloLaura AnfossiSimone CavaleraFabio Di NardoThea SerraClaudio BaggianiMDPI AGarticlemolecularly imprinted polymernanoMIPsolid phase synthesisMISPEsolid phase extractionfluoroquinolonePhysicsQC1-999ChemistryQD1-999ENSeparations, Vol 8, Iss 226, p 226 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	molecularly imprinted polymer nanoMIP solid phase synthesis MISPE solid phase extraction fluoroquinolone Physics QC1-999 Chemistry QD1-999
spellingShingle	molecularly imprinted polymer nanoMIP solid phase synthesis MISPE solid phase extraction fluoroquinolone Physics QC1-999 Chemistry QD1-999 Matteo Chiarello Laura Anfossi Simone Cavalera Fabio Di Nardo Thea Serra Claudio Baggiani NanoMIP-Based Solid Phase Extraction of Fluoroquinolones from Human Urine: A Proof-of-Concept Study
description	NanoMIPs that are prepared by solid phase synthesis have proven to be very versatile, but to date only limited attention has been paid to their use in solid phase extraction. Thus, since nanoMIPs show close similarities, in terms of binding behavior, to antibodies, it seems relevant to verify if it is possible to use them as mimics of the natural antibodies that are used in immunoextraction methods. As a proof-of-concept, we considered prepared nanoMIPs against fluoroquinolone ciprofloxacin. Several nanoMIPs were prepared in water with polymerization mixtures of different compositions. The polymer with the highest affinity towards ciprofloxacin was then grafted onto a solid support and used to set up a solid phase extraction–HPLC method with fluorescence detection, for the determination of fluoroquinolones in human urine. The method resulted in successful selection for the fluoroquinolone antibiotics, such that the nanoMIPs were suitable for direct extraction of the antibiotics from the urine samples at the µg mL<sup>−1</sup> level. They required no preliminary treatment, except for a 1 + 9 (<i>v</i>/<i>v</i>) dilution with a buffer of pH 4.5 and they had good analyte recovery rates; up to 85% with precision in the range of 3 to 4.5%, without interference from the matrix. These experimental results demonstrate, for the first time, the feasibility of the use of nanoMIPs to develop solid phase extraction methods.
format	article
author	Matteo Chiarello Laura Anfossi Simone Cavalera Fabio Di Nardo Thea Serra Claudio Baggiani
author_facet	Matteo Chiarello Laura Anfossi Simone Cavalera Fabio Di Nardo Thea Serra Claudio Baggiani
author_sort	Matteo Chiarello
title	NanoMIP-Based Solid Phase Extraction of Fluoroquinolones from Human Urine: A Proof-of-Concept Study
title_short	NanoMIP-Based Solid Phase Extraction of Fluoroquinolones from Human Urine: A Proof-of-Concept Study
title_full	NanoMIP-Based Solid Phase Extraction of Fluoroquinolones from Human Urine: A Proof-of-Concept Study
title_fullStr	NanoMIP-Based Solid Phase Extraction of Fluoroquinolones from Human Urine: A Proof-of-Concept Study
title_full_unstemmed	NanoMIP-Based Solid Phase Extraction of Fluoroquinolones from Human Urine: A Proof-of-Concept Study
title_sort	nanomip-based solid phase extraction of fluoroquinolones from human urine: a proof-of-concept study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d840eccdbd354d498c29f5c7347042fd
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NanoMIP-Based Solid Phase Extraction of Fluoroquinolones from Human Urine: A Proof-of-Concept Study

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