Effects of SDF-1/CXCR7 on the Migration, Invasion and Epithelial-Mesenchymal Transition of Gastric Cancer Cells

We found that SDF-1/CXCR7 axis played an important role in the growth and proliferation of gastric cancer in the previous studies. The objectives of this study were to explore the effects of SDF-1/CXCR7 on the metastatic ability of gastric cancer cells and the possible mechanisms. CXCR7 expression i...

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Autores principales:	Ameng Shi, Ting Wang, Miao Jia, Lei Dong, Haitao Shi
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	chemokine (CXC motif) receptor 7 (CXCR7) migration invasion epithelial-mesenchymal transition (EMT) gastric cancer Genetics QH426-470
Acceso en línea:	https://doaj.org/article/d84cea52f9614fe4b7d28e2d8e7ce169
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spelling	oai:doaj.org-article:d84cea52f9614fe4b7d28e2d8e7ce1692021-11-09T06:08:03ZEffects of SDF-1/CXCR7 on the Migration, Invasion and Epithelial-Mesenchymal Transition of Gastric Cancer Cells1664-802110.3389/fgene.2021.760048https://doaj.org/article/d84cea52f9614fe4b7d28e2d8e7ce1692021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.760048/fullhttps://doaj.org/toc/1664-8021We found that SDF-1/CXCR7 axis played an important role in the growth and proliferation of gastric cancer in the previous studies. The objectives of this study were to explore the effects of SDF-1/CXCR7 on the metastatic ability of gastric cancer cells and the possible mechanisms. CXCR7 expression in SGC-7901 gastric cancer cells was stably knocked down via lentiviral vectors. The cell migration and invasion abilities were detected by transwell migration and invasion assays. The expressions of matrix metalloproteinase 2 (MMP-2), MMP-9, vascular endothelial growth factor (VEGF), epithelial-mesenchymal transition (EMT) markers and Akt phosphorylation were detected with real-time PCR and/or western blot. We found that SDF-1 markedly enhanced the migration and invasion abilities of SGC-7901 gastric cancer cells; CXCR7 knockdown inhibited these effects. SDF-1/CXCR7 increased the expressions of MMP-2, MMP-9 and VEGF. SDF-1/CXCR7 also downregulated E-cadherin expression but upregulated N-cadherin, vimentin and Snail expressions, suggesting that SDF-1/CXCR7 could promote the development of EMT in gastric cancer cells. Furthermore, SDF-1/CXCR7 could promote Akt phosphorylation. Our results indicated that SDF-1/CXCR7 enhanced the migration, invasion and EMT of gastric cancer cells and thus CXCR7 supression may be a strategy for inhibiting gastric cancer metastasis.Ameng ShiTing WangMiao JiaLei DongHaitao ShiFrontiers Media S.A.articlechemokine (CXC motif) receptor 7 (CXCR7)migrationinvasionepithelial-mesenchymal transition (EMT)gastric cancerGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chemokine (CXC motif) receptor 7 (CXCR7) migration invasion epithelial-mesenchymal transition (EMT) gastric cancer Genetics QH426-470
spellingShingle	chemokine (CXC motif) receptor 7 (CXCR7) migration invasion epithelial-mesenchymal transition (EMT) gastric cancer Genetics QH426-470 Ameng Shi Ting Wang Miao Jia Lei Dong Haitao Shi Effects of SDF-1/CXCR7 on the Migration, Invasion and Epithelial-Mesenchymal Transition of Gastric Cancer Cells
description	We found that SDF-1/CXCR7 axis played an important role in the growth and proliferation of gastric cancer in the previous studies. The objectives of this study were to explore the effects of SDF-1/CXCR7 on the metastatic ability of gastric cancer cells and the possible mechanisms. CXCR7 expression in SGC-7901 gastric cancer cells was stably knocked down via lentiviral vectors. The cell migration and invasion abilities were detected by transwell migration and invasion assays. The expressions of matrix metalloproteinase 2 (MMP-2), MMP-9, vascular endothelial growth factor (VEGF), epithelial-mesenchymal transition (EMT) markers and Akt phosphorylation were detected with real-time PCR and/or western blot. We found that SDF-1 markedly enhanced the migration and invasion abilities of SGC-7901 gastric cancer cells; CXCR7 knockdown inhibited these effects. SDF-1/CXCR7 increased the expressions of MMP-2, MMP-9 and VEGF. SDF-1/CXCR7 also downregulated E-cadherin expression but upregulated N-cadherin, vimentin and Snail expressions, suggesting that SDF-1/CXCR7 could promote the development of EMT in gastric cancer cells. Furthermore, SDF-1/CXCR7 could promote Akt phosphorylation. Our results indicated that SDF-1/CXCR7 enhanced the migration, invasion and EMT of gastric cancer cells and thus CXCR7 supression may be a strategy for inhibiting gastric cancer metastasis.
format	article
author	Ameng Shi Ting Wang Miao Jia Lei Dong Haitao Shi
author_facet	Ameng Shi Ting Wang Miao Jia Lei Dong Haitao Shi
author_sort	Ameng Shi
title	Effects of SDF-1/CXCR7 on the Migration, Invasion and Epithelial-Mesenchymal Transition of Gastric Cancer Cells
title_short	Effects of SDF-1/CXCR7 on the Migration, Invasion and Epithelial-Mesenchymal Transition of Gastric Cancer Cells
title_full	Effects of SDF-1/CXCR7 on the Migration, Invasion and Epithelial-Mesenchymal Transition of Gastric Cancer Cells
title_fullStr	Effects of SDF-1/CXCR7 on the Migration, Invasion and Epithelial-Mesenchymal Transition of Gastric Cancer Cells
title_full_unstemmed	Effects of SDF-1/CXCR7 on the Migration, Invasion and Epithelial-Mesenchymal Transition of Gastric Cancer Cells
title_sort	effects of sdf-1/cxcr7 on the migration, invasion and epithelial-mesenchymal transition of gastric cancer cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d84cea52f9614fe4b7d28e2d8e7ce169
work_keys_str_mv	AT amengshi effectsofsdf1cxcr7onthemigrationinvasionandepithelialmesenchymaltransitionofgastriccancercells AT tingwang effectsofsdf1cxcr7onthemigrationinvasionandepithelialmesenchymaltransitionofgastriccancercells AT miaojia effectsofsdf1cxcr7onthemigrationinvasionandepithelialmesenchymaltransitionofgastriccancercells AT leidong effectsofsdf1cxcr7onthemigrationinvasionandepithelialmesenchymaltransitionofgastriccancercells AT haitaoshi effectsofsdf1cxcr7onthemigrationinvasionandepithelialmesenchymaltransitionofgastriccancercells
_version_	1718441337917800448

Effects of SDF-1/CXCR7 on the Migration, Invasion and Epithelial-Mesenchymal Transition of Gastric Cancer Cells

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