Immunoglobulin subtype-coated bacteria are correlated with the disease activity of inflammatory bowel disease

Abstract Immune response involving various immunoglobulin (Ig) isotypes and subtypes to microbiome is involved in the pathogenesis and disease activity of inflammatory bowel diseases (IBDs). To clarify the presence of Ig-coated bacteria in the intestine and its association with disease activity in u...

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Autores principales:	Yutaro Masu, Yoshitake Kanazawa, Yoichi Kakuta, Yusuke Shimoyama, Motoyuki Onodera, Takeo Naito, Rintaro Moroi, Masatake Kuroha, Tomoya Kimura, Hisashi Shiga, Yoshitaka Kinouchi, Atsushi Masamune
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d84d1fc0ae324cd7bd7d37a982b675dd
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spelling	oai:doaj.org-article:d84d1fc0ae324cd7bd7d37a982b675dd2021-12-02T17:08:44ZImmunoglobulin subtype-coated bacteria are correlated with the disease activity of inflammatory bowel disease10.1038/s41598-021-96289-52045-2322https://doaj.org/article/d84d1fc0ae324cd7bd7d37a982b675dd2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96289-5https://doaj.org/toc/2045-2322Abstract Immune response involving various immunoglobulin (Ig) isotypes and subtypes to microbiome is involved in the pathogenesis and disease activity of inflammatory bowel diseases (IBDs). To clarify the presence of Ig-coated bacteria in the intestine and its association with disease activity in ulcerative colitis (UC) and Crohn’s disease (CD), we extracted and classified Ig-coated bacteria from fecal samples of 42 patients with IBD and 12 healthy controls (HCs) using flow cytometry and 16S ribosomal RNA sequence analysis. The percentage of bacteria coated with IgA and IgM was higher in patients with IBD than in HCs, and IgG-coated bacteria were found only in patients with IBD. Moreover, the percentages of bacteria coated with IgG1, IgG2, IgG3, and IgM in UC samples and IgG3, IgG4, and IgM in CD samples were correlated with disease activities. The proportions of Bacteroides ovatus and Streptococcus increased during the active phase of CD. Hence, the detailed analysis of Ig-coated bacteria and Ig subtypes using flow cytometry could aid in developing useful indicators of disease activity and identifying more disease-related bacteria, which could become novel treatment targets for IBDs.Yutaro MasuYoshitake KanazawaYoichi KakutaYusuke ShimoyamaMotoyuki OnoderaTakeo NaitoRintaro MoroiMasatake KurohaTomoya KimuraHisashi ShigaYoshitaka KinouchiAtsushi MasamuneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yutaro Masu Yoshitake Kanazawa Yoichi Kakuta Yusuke Shimoyama Motoyuki Onodera Takeo Naito Rintaro Moroi Masatake Kuroha Tomoya Kimura Hisashi Shiga Yoshitaka Kinouchi Atsushi Masamune Immunoglobulin subtype-coated bacteria are correlated with the disease activity of inflammatory bowel disease
description	Abstract Immune response involving various immunoglobulin (Ig) isotypes and subtypes to microbiome is involved in the pathogenesis and disease activity of inflammatory bowel diseases (IBDs). To clarify the presence of Ig-coated bacteria in the intestine and its association with disease activity in ulcerative colitis (UC) and Crohn’s disease (CD), we extracted and classified Ig-coated bacteria from fecal samples of 42 patients with IBD and 12 healthy controls (HCs) using flow cytometry and 16S ribosomal RNA sequence analysis. The percentage of bacteria coated with IgA and IgM was higher in patients with IBD than in HCs, and IgG-coated bacteria were found only in patients with IBD. Moreover, the percentages of bacteria coated with IgG1, IgG2, IgG3, and IgM in UC samples and IgG3, IgG4, and IgM in CD samples were correlated with disease activities. The proportions of Bacteroides ovatus and Streptococcus increased during the active phase of CD. Hence, the detailed analysis of Ig-coated bacteria and Ig subtypes using flow cytometry could aid in developing useful indicators of disease activity and identifying more disease-related bacteria, which could become novel treatment targets for IBDs.
format	article
author	Yutaro Masu Yoshitake Kanazawa Yoichi Kakuta Yusuke Shimoyama Motoyuki Onodera Takeo Naito Rintaro Moroi Masatake Kuroha Tomoya Kimura Hisashi Shiga Yoshitaka Kinouchi Atsushi Masamune
author_facet	Yutaro Masu Yoshitake Kanazawa Yoichi Kakuta Yusuke Shimoyama Motoyuki Onodera Takeo Naito Rintaro Moroi Masatake Kuroha Tomoya Kimura Hisashi Shiga Yoshitaka Kinouchi Atsushi Masamune
author_sort	Yutaro Masu
title	Immunoglobulin subtype-coated bacteria are correlated with the disease activity of inflammatory bowel disease
title_short	Immunoglobulin subtype-coated bacteria are correlated with the disease activity of inflammatory bowel disease
title_full	Immunoglobulin subtype-coated bacteria are correlated with the disease activity of inflammatory bowel disease
title_fullStr	Immunoglobulin subtype-coated bacteria are correlated with the disease activity of inflammatory bowel disease
title_full_unstemmed	Immunoglobulin subtype-coated bacteria are correlated with the disease activity of inflammatory bowel disease
title_sort	immunoglobulin subtype-coated bacteria are correlated with the disease activity of inflammatory bowel disease
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d84d1fc0ae324cd7bd7d37a982b675dd
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Immunoglobulin subtype-coated bacteria are correlated with the disease activity of inflammatory bowel disease

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