Comprehensive Compositional Analysis of the Slit Lamp Bacteriota

Slit lamps are routinely used to examine large numbers of patients every day due to high throughput. Previous, cultivation-based results suggested slit lamps to be contaminated with bacteria, mostly coagulase-negative staphylococci, followed by micrococci, bacilli, but also Staphylococcus aureus. Ou...

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Autores principales:	Birgit Fritz, Edita Paschko, Wayne Young, Daniel Böhringer, Siegfried Wahl, Focke Ziemssen, Markus Egert
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	eye hygiene MRSA Illumina MiSeq ® microbiota (16S) 16S rRNA gene amplicon sequencing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d84d778db65946469ed67f93c2d5bdd0
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spelling	oai:doaj.org-article:d84d778db65946469ed67f93c2d5bdd02021-11-17T05:51:59ZComprehensive Compositional Analysis of the Slit Lamp Bacteriota2235-298810.3389/fcimb.2021.745653https://doaj.org/article/d84d778db65946469ed67f93c2d5bdd02021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.745653/fullhttps://doaj.org/toc/2235-2988Slit lamps are routinely used to examine large numbers of patients every day due to high throughput. Previous, cultivation-based results suggested slit lamps to be contaminated with bacteria, mostly coagulase-negative staphylococci, followed by micrococci, bacilli, but also Staphylococcus aureus. Our study aimed at obtaining a much more comprehensive, cultivation-independent view of the slit lamp bacteriota and its hygienic relevance, as regularly touched surfaces usually represent fomites, particularly if used by different persons. We performed extensive 16S rRNA gene sequencing to analyse the bacteriota, of 46 slit lamps from two tertiary care centers at two sampling sites, respectively. 82 samples yielded enough sequences for downstream analyses and revealed contamination with bacteria of mostly human skin, mucosa and probably eye origin, predominantly cutibacteria, staphylococci and corynebacteria. The taxonomic assignment of 3369 ASVs (amplicon sequence variants) revealed 19 bacterial phyla and 468 genera across all samples. As antibiotic resistances are of major concern, we screened all samples for methicillin-resistant Staphylococcus aureus (MRSA) using qPCR, however, no signals above the detection limit were detected. Our study provides first comprehensive insight into the slit lamp microbiota. It underlines that slit lamps carry a highly diverse, skin-like bacterial microbiota and that thorough cleaning and disinfection after use is highly recommendable to prevent eye and skin infections.Birgit FritzEdita PaschkoWayne YoungDaniel BöhringerSiegfried WahlSiegfried WahlFocke ZiemssenMarkus EgertFrontiers Media S.A.articleeyehygieneMRSAIllumina MiSeq ®microbiota (16S)16S rRNA gene amplicon sequencingMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	eye hygiene MRSA Illumina MiSeq ® microbiota (16S) 16S rRNA gene amplicon sequencing Microbiology QR1-502
spellingShingle	eye hygiene MRSA Illumina MiSeq ® microbiota (16S) 16S rRNA gene amplicon sequencing Microbiology QR1-502 Birgit Fritz Edita Paschko Wayne Young Daniel Böhringer Siegfried Wahl Siegfried Wahl Focke Ziemssen Markus Egert Comprehensive Compositional Analysis of the Slit Lamp Bacteriota
description	Slit lamps are routinely used to examine large numbers of patients every day due to high throughput. Previous, cultivation-based results suggested slit lamps to be contaminated with bacteria, mostly coagulase-negative staphylococci, followed by micrococci, bacilli, but also Staphylococcus aureus. Our study aimed at obtaining a much more comprehensive, cultivation-independent view of the slit lamp bacteriota and its hygienic relevance, as regularly touched surfaces usually represent fomites, particularly if used by different persons. We performed extensive 16S rRNA gene sequencing to analyse the bacteriota, of 46 slit lamps from two tertiary care centers at two sampling sites, respectively. 82 samples yielded enough sequences for downstream analyses and revealed contamination with bacteria of mostly human skin, mucosa and probably eye origin, predominantly cutibacteria, staphylococci and corynebacteria. The taxonomic assignment of 3369 ASVs (amplicon sequence variants) revealed 19 bacterial phyla and 468 genera across all samples. As antibiotic resistances are of major concern, we screened all samples for methicillin-resistant Staphylococcus aureus (MRSA) using qPCR, however, no signals above the detection limit were detected. Our study provides first comprehensive insight into the slit lamp microbiota. It underlines that slit lamps carry a highly diverse, skin-like bacterial microbiota and that thorough cleaning and disinfection after use is highly recommendable to prevent eye and skin infections.
format	article
author	Birgit Fritz Edita Paschko Wayne Young Daniel Böhringer Siegfried Wahl Siegfried Wahl Focke Ziemssen Markus Egert
author_facet	Birgit Fritz Edita Paschko Wayne Young Daniel Böhringer Siegfried Wahl Siegfried Wahl Focke Ziemssen Markus Egert
author_sort	Birgit Fritz
title	Comprehensive Compositional Analysis of the Slit Lamp Bacteriota
title_short	Comprehensive Compositional Analysis of the Slit Lamp Bacteriota
title_full	Comprehensive Compositional Analysis of the Slit Lamp Bacteriota
title_fullStr	Comprehensive Compositional Analysis of the Slit Lamp Bacteriota
title_full_unstemmed	Comprehensive Compositional Analysis of the Slit Lamp Bacteriota
title_sort	comprehensive compositional analysis of the slit lamp bacteriota
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d84d778db65946469ed67f93c2d5bdd0
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Comprehensive Compositional Analysis of the Slit Lamp Bacteriota

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