Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents

Abstract The DNA demethylating agent, 5-Azacytidine (5Aza), and histone deacetylase inhibitor, valproic acid (Vpa), can improve the reprogramming efficiencies of pluripotent cells. This study aimed to examine the roles of 5Aza and Vpa in the dedifferentiation of epithelial cell rests of Malassez (ER...

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Autores principales:	Koki Yoshida, Osamu Uehara, Yoshihito Kurashige, Durga Paudel, Aya Onishi, Puja Neopane, Daichi Hiraki, Tetsuro Morikawa, Fumiya Harada, Rie Takai, Jun Sato, Masato Saitoh, Yoshihiro Abiko
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d84d9fdd546a47f8b5875b4315730b1b
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spelling	oai:doaj.org-article:d84d9fdd546a47f8b5875b4315730b1b2021-12-02T10:49:11ZDirect reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents10.1038/s41598-020-79426-42045-2322https://doaj.org/article/d84d9fdd546a47f8b5875b4315730b1b2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79426-4https://doaj.org/toc/2045-2322Abstract The DNA demethylating agent, 5-Azacytidine (5Aza), and histone deacetylase inhibitor, valproic acid (Vpa), can improve the reprogramming efficiencies of pluripotent cells. This study aimed to examine the roles of 5Aza and Vpa in the dedifferentiation of epithelial cell rests of Malassez (ERM) into stem-like cells. Additionally, the ability of stem-like cells to differentiate into mesenchymal cells was evaluated. ERM was cultured in embryonic stem cell medium (ESCM) with 1 µM of 5Aza, or 2 mM of Vpa, or a combination of 5Aza and Vpa. The cells stimulated with both 5Aza and Vpa were named as progenitor-dedifferentiated into stem-like cells (Pro-DSLCs). The Pro-DSLCs cultured in ESCM alone for another week were named as DSLCs. The stem cell markers were significantly higher in the DSLCs than the controls (no additions). The mRNA and protein levels of the endothelial, mesenchymal stem, and osteogenic cell markers were significantly higher in the Pro-DSLCs and DSLCs than the controls. The combination of a demethylating agent and a deacetylated inhibitor induced the dedifferentiation of ERM into DSLCs. The Pro-DSLCs derived from ERM can be directly reprogrammed into mesenchymal-like cells without dedifferentiation into stem-like cells. Isolated ERM treated with epigenetic agents may be used for periodontal regeneration.Koki YoshidaOsamu UeharaYoshihito KurashigeDurga PaudelAya OnishiPuja NeopaneDaichi HirakiTetsuro MorikawaFumiya HaradaRie TakaiJun SatoMasato SaitohYoshihiro AbikoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Koki Yoshida Osamu Uehara Yoshihito Kurashige Durga Paudel Aya Onishi Puja Neopane Daichi Hiraki Tetsuro Morikawa Fumiya Harada Rie Takai Jun Sato Masato Saitoh Yoshihiro Abiko Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents
description	Abstract The DNA demethylating agent, 5-Azacytidine (5Aza), and histone deacetylase inhibitor, valproic acid (Vpa), can improve the reprogramming efficiencies of pluripotent cells. This study aimed to examine the roles of 5Aza and Vpa in the dedifferentiation of epithelial cell rests of Malassez (ERM) into stem-like cells. Additionally, the ability of stem-like cells to differentiate into mesenchymal cells was evaluated. ERM was cultured in embryonic stem cell medium (ESCM) with 1 µM of 5Aza, or 2 mM of Vpa, or a combination of 5Aza and Vpa. The cells stimulated with both 5Aza and Vpa were named as progenitor-dedifferentiated into stem-like cells (Pro-DSLCs). The Pro-DSLCs cultured in ESCM alone for another week were named as DSLCs. The stem cell markers were significantly higher in the DSLCs than the controls (no additions). The mRNA and protein levels of the endothelial, mesenchymal stem, and osteogenic cell markers were significantly higher in the Pro-DSLCs and DSLCs than the controls. The combination of a demethylating agent and a deacetylated inhibitor induced the dedifferentiation of ERM into DSLCs. The Pro-DSLCs derived from ERM can be directly reprogrammed into mesenchymal-like cells without dedifferentiation into stem-like cells. Isolated ERM treated with epigenetic agents may be used for periodontal regeneration.
format	article
author	Koki Yoshida Osamu Uehara Yoshihito Kurashige Durga Paudel Aya Onishi Puja Neopane Daichi Hiraki Tetsuro Morikawa Fumiya Harada Rie Takai Jun Sato Masato Saitoh Yoshihiro Abiko
author_facet	Koki Yoshida Osamu Uehara Yoshihito Kurashige Durga Paudel Aya Onishi Puja Neopane Daichi Hiraki Tetsuro Morikawa Fumiya Harada Rie Takai Jun Sato Masato Saitoh Yoshihiro Abiko
author_sort	Koki Yoshida
title	Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents
title_short	Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents
title_full	Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents
title_fullStr	Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents
title_full_unstemmed	Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents
title_sort	direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d84d9fdd546a47f8b5875b4315730b1b
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Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents

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