Enhanced cellulase enzyme production by Aspergillus niger using cellulase/iron oxide magnetic nano-composites

Objectives: The cellulase enzyme is used for various industrial applications such as textile, paper, food and biofuel industries. Industrially, the fungal strain, Aspergillus niger, is widely used to produce cellulase enzymes. Emerging evidence has indicated the possible role of magnetic nanocomposi...

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Autores principales:	Durairaj Siva, Gunasekaran Srivethi, Poovanalingam Thirumalai Vasan, Durairaj Rajesh, Ahmed Alfarhan, Rajakrishnan Rajagopal
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Cellulase Cellulase/iron oxide magnetic composites Carboxymethyl cellulose assay Filter paper assay Aspergillus niger Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/d850fda0f6eb4aa09aedd8124447dc8e
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spelling	oai:doaj.org-article:d850fda0f6eb4aa09aedd8124447dc8e2021-11-22T04:18:45ZEnhanced cellulase enzyme production by Aspergillus niger using cellulase/iron oxide magnetic nano-composites1018-364710.1016/j.jksus.2021.101695https://doaj.org/article/d850fda0f6eb4aa09aedd8124447dc8e2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721003578https://doaj.org/toc/1018-3647Objectives: The cellulase enzyme is used for various industrial applications such as textile, paper, food and biofuel industries. Industrially, the fungal strain, Aspergillus niger, is widely used to produce cellulase enzymes. Emerging evidence has indicated the possible role of magnetic nanocomposites in enhancing cellulase production by A. niger. The cellulase/iron oxide nanocomposites are already reported to be an eco-friendly method for modulating microbial biochemical characteristics. Methods: The present study was used to assess the efficacy of cellulase/iron oxide magnetic composites (CMNPS) for the enhanced production of cellulase enzyme. The fungal strain Aspergillus niger was isolated from the soil samples by using standard techniques. The fungi were then transfected with CMNPS and further evaluated for the production of cellulase enzyme. Results: Synthesis of the cellulase/iron oxide magnetic composites was characterized by UV, FTIR, and XRD. The produced CMNPS was used as substrate and enzyme production. The cellulase enzyme production by Aspergillus niger was analyzed by CMC (0.82 IU/ml enzyme activity) and FPA assay (0.039 IU/ml enzyme activity). CMNPS was also analyzed by CMC (0.74 IU/ml enzyme activity) and FPA assay (0.039 IU/ml enzyme activity). Reuse of CMNPS is the ultimate application and ecofriendly approach for the synthesis of cellulase enzymes. Conclusion: The study concludes that the cellulase/ iron oxide nanocomposites may be a useful tool for the enhanced production of cellulase enzyme from the soil fungus A. niger.Durairaj SivaGunasekaran SrivethiPoovanalingam Thirumalai VasanDurairaj RajeshAhmed AlfarhanRajakrishnan RajagopalElsevierarticleCellulaseCellulase/iron oxide magnetic compositesCarboxymethyl cellulose assayFilter paper assayAspergillus nigerScience (General)Q1-390ENJournal of King Saud University: Science, Vol 34, Iss 1, Pp 101695- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cellulase Cellulase/iron oxide magnetic composites Carboxymethyl cellulose assay Filter paper assay Aspergillus niger Science (General) Q1-390
spellingShingle	Cellulase Cellulase/iron oxide magnetic composites Carboxymethyl cellulose assay Filter paper assay Aspergillus niger Science (General) Q1-390 Durairaj Siva Gunasekaran Srivethi Poovanalingam Thirumalai Vasan Durairaj Rajesh Ahmed Alfarhan Rajakrishnan Rajagopal Enhanced cellulase enzyme production by Aspergillus niger using cellulase/iron oxide magnetic nano-composites
description	Objectives: The cellulase enzyme is used for various industrial applications such as textile, paper, food and biofuel industries. Industrially, the fungal strain, Aspergillus niger, is widely used to produce cellulase enzymes. Emerging evidence has indicated the possible role of magnetic nanocomposites in enhancing cellulase production by A. niger. The cellulase/iron oxide nanocomposites are already reported to be an eco-friendly method for modulating microbial biochemical characteristics. Methods: The present study was used to assess the efficacy of cellulase/iron oxide magnetic composites (CMNPS) for the enhanced production of cellulase enzyme. The fungal strain Aspergillus niger was isolated from the soil samples by using standard techniques. The fungi were then transfected with CMNPS and further evaluated for the production of cellulase enzyme. Results: Synthesis of the cellulase/iron oxide magnetic composites was characterized by UV, FTIR, and XRD. The produced CMNPS was used as substrate and enzyme production. The cellulase enzyme production by Aspergillus niger was analyzed by CMC (0.82 IU/ml enzyme activity) and FPA assay (0.039 IU/ml enzyme activity). CMNPS was also analyzed by CMC (0.74 IU/ml enzyme activity) and FPA assay (0.039 IU/ml enzyme activity). Reuse of CMNPS is the ultimate application and ecofriendly approach for the synthesis of cellulase enzymes. Conclusion: The study concludes that the cellulase/ iron oxide nanocomposites may be a useful tool for the enhanced production of cellulase enzyme from the soil fungus A. niger.
format	article
author	Durairaj Siva Gunasekaran Srivethi Poovanalingam Thirumalai Vasan Durairaj Rajesh Ahmed Alfarhan Rajakrishnan Rajagopal
author_facet	Durairaj Siva Gunasekaran Srivethi Poovanalingam Thirumalai Vasan Durairaj Rajesh Ahmed Alfarhan Rajakrishnan Rajagopal
author_sort	Durairaj Siva
title	Enhanced cellulase enzyme production by Aspergillus niger using cellulase/iron oxide magnetic nano-composites
title_short	Enhanced cellulase enzyme production by Aspergillus niger using cellulase/iron oxide magnetic nano-composites
title_full	Enhanced cellulase enzyme production by Aspergillus niger using cellulase/iron oxide magnetic nano-composites
title_fullStr	Enhanced cellulase enzyme production by Aspergillus niger using cellulase/iron oxide magnetic nano-composites
title_full_unstemmed	Enhanced cellulase enzyme production by Aspergillus niger using cellulase/iron oxide magnetic nano-composites
title_sort	enhanced cellulase enzyme production by aspergillus niger using cellulase/iron oxide magnetic nano-composites
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/d850fda0f6eb4aa09aedd8124447dc8e
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Enhanced cellulase enzyme production by Aspergillus niger using cellulase/iron oxide magnetic nano-composites

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