Recombinant epidermal growth factor-like domain-1 from coagulation factor VII functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging

Heng Liu,1,2 Xiao Chen,1 Wei Xue,1 Chengchao Chu,2 Yu Liu,2 Haipeng Tong,1 Xuesong Du,1 Tian Xie,1 Gang Liu,2 Weiguo Zhang1,3 1Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 2State Key Laboratory of Molecular Vaccinology and Mo...

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Autores principales:	Liu H, Chen X, Xue W, Chu C, Liu Y, Tong HP, Du X, Xie T, Liu G, Zhang WG
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	epidermal growth factor-like domain-1 tissue factor iron oxide nanoparticles MRI glioma Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d85360251aa944819bef8daa85dd2e0c
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spelling	oai:doaj.org-article:d85360251aa944819bef8daa85dd2e0c2021-12-02T07:15:23ZRecombinant epidermal growth factor-like domain-1 from coagulation factor VII functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging1178-2013https://doaj.org/article/d85360251aa944819bef8daa85dd2e0c2016-10-01T00:00:00Zhttps://www.dovepress.com/recombinant-epidermal-growth-factor-like-domain-1-from-coagulation-fac-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Heng Liu,1,2 Xiao Chen,1 Wei Xue,1 Chengchao Chu,2 Yu Liu,2 Haipeng Tong,1 Xuesong Du,1 Tian Xie,1 Gang Liu,2 Weiguo Zhang1,3 1Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 2State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, Fujian, 3Chongqing Clinical Research Center for Imaging and Nuclear Medicine, Chongqing, People’s Republic of China Abstract: The highly infiltrative and invasive nature of glioma cells often leads to blurred tumor margins, resulting in incomplete tumor resection and tumor recurrence. Accurate detection and precise delineation of glioma help in preoperative delineation, surgical planning and survival prediction. In this study, recombinant epidermal growth factor-like domain-1, derived from human coagulation factor VII, was conjugated to iron oxide nanoparticles (IONPs) for targeted glioma magnetic resonance (MR) imaging. The synthesized EGF1-EGFP-IONPs exhibited excellent targeting ability toward tissue factor (TF)-positive U87MG cells and human umbilical vein endothelial cells in vitro, and demonstrated persistent and efficient MR contrast enhancement up to 12 h for preclinical glioma models with high targeting specificity in vivo. They hold great potential for clinical translation and developing targeted theranostics against brain glioma. Keywords: epidermal growth factor-like domain-1, tissue factor, iron oxide nanoparticles, MRI, gliomaLiu HChen XXue WChu CLiu YTong HPDu XXie TLiu GZhang WGDove Medical Pressarticleepidermal growth factor-like domain-1tissue factoriron oxide nanoparticlesMRIgliomaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5099-5108 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	epidermal growth factor-like domain-1 tissue factor iron oxide nanoparticles MRI glioma Medicine (General) R5-920
spellingShingle	epidermal growth factor-like domain-1 tissue factor iron oxide nanoparticles MRI glioma Medicine (General) R5-920 Liu H Chen X Xue W Chu C Liu Y Tong HP Du X Xie T Liu G Zhang WG Recombinant epidermal growth factor-like domain-1 from coagulation factor VII functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging
description	Heng Liu,1,2 Xiao Chen,1 Wei Xue,1 Chengchao Chu,2 Yu Liu,2 Haipeng Tong,1 Xuesong Du,1 Tian Xie,1 Gang Liu,2 Weiguo Zhang1,3 1Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 2State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, Fujian, 3Chongqing Clinical Research Center for Imaging and Nuclear Medicine, Chongqing, People’s Republic of China Abstract: The highly infiltrative and invasive nature of glioma cells often leads to blurred tumor margins, resulting in incomplete tumor resection and tumor recurrence. Accurate detection and precise delineation of glioma help in preoperative delineation, surgical planning and survival prediction. In this study, recombinant epidermal growth factor-like domain-1, derived from human coagulation factor VII, was conjugated to iron oxide nanoparticles (IONPs) for targeted glioma magnetic resonance (MR) imaging. The synthesized EGF1-EGFP-IONPs exhibited excellent targeting ability toward tissue factor (TF)-positive U87MG cells and human umbilical vein endothelial cells in vitro, and demonstrated persistent and efficient MR contrast enhancement up to 12 h for preclinical glioma models with high targeting specificity in vivo. They hold great potential for clinical translation and developing targeted theranostics against brain glioma. Keywords: epidermal growth factor-like domain-1, tissue factor, iron oxide nanoparticles, MRI, glioma
format	article
author	Liu H Chen X Xue W Chu C Liu Y Tong HP Du X Xie T Liu G Zhang WG
author_facet	Liu H Chen X Xue W Chu C Liu Y Tong HP Du X Xie T Liu G Zhang WG
author_sort	Liu H
title	Recombinant epidermal growth factor-like domain-1 from coagulation factor VII functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging
title_short	Recombinant epidermal growth factor-like domain-1 from coagulation factor VII functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging
title_full	Recombinant epidermal growth factor-like domain-1 from coagulation factor VII functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging
title_fullStr	Recombinant epidermal growth factor-like domain-1 from coagulation factor VII functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging
title_full_unstemmed	Recombinant epidermal growth factor-like domain-1 from coagulation factor VII functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging
title_sort	recombinant epidermal growth factor-like domain-1 from coagulation factor vii functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/d85360251aa944819bef8daa85dd2e0c
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Recombinant epidermal growth factor-like domain-1 from coagulation factor VII functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging

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