INFLUENCE OF HLA-DRB1* ALLELIC SETS ON THE DEVELOPMENT OF TUBERCULOSIS IN CHILDREN

According to the WHO data, tuberculosis still represents a serious public health problem worldwide. Deterioration of socio-economic conditions in the population complicates epidemic situation for tuberculosis inRussia, thus leading to increase in acute progressive and complicated forms of tuberculos...

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Autores principales:	A. A. Starshinova, N. V. Korneva, I. F. Dovgalyuk, M. V. Pavlova, I. E. Pavlova, L. N. Bubnova
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2015
Materias:	children diagnostic immunogenetic tuberculosis hla-drb1 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/d85787992cad458ea6f40cc1750e991f
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spelling	oai:doaj.org-article:d85787992cad458ea6f40cc1750e991f2021-11-18T08:03:44ZINFLUENCE OF HLA-DRB1* ALLELIC SETS ON THE DEVELOPMENT OF TUBERCULOSIS IN CHILDREN1563-06252313-741X10.15789/1563-0625-2015-3-275-280https://doaj.org/article/d85787992cad458ea6f40cc1750e991f2015-06-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/893https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAccording to the WHO data, tuberculosis still represents a serious public health problem worldwide. Deterioration of socio-economic conditions in the population complicates epidemic situation for tuberculosis inRussia, thus leading to increase in acute progressive and complicated forms of tuberculosis in children and, consequently, to worsening structure of its clinical forms. Objectives: to determine associations between certain HLA-DRB1 alleles and risk of tuberculosis development in children. We examined 188 children aged from 3 to 14 years with various manifestations of tuberculous infection. Along with thorough examination of the patients, including multi-spiral CT scans of chest, we undertook genotyping of HLA-DRB1 alleles. Activity of tuberculous infection was determined by a set of immunological tests, i.e., tuberculin skin test, DIASKINTEST® (recombinant allergen of tuberculosis DIASKINTEST®). X ray diagnostics was performed with multi-spiral «Aquilion-32» computed tomograph (Toshiba), according to standard procedures. Molecular genetic typing of HLA-DRB1 alleles was performed by polymerase chain reaction (PCR-SSP), using standard commercial kits PROTRANS Ceclerplate System Protrans HLA-DRB1. The children were divided into two groups: I group, 90 healthy children, II group, 98 children with tuberculosis. A comparisons group consisted of healthy donors (n = 346). Statistical processing of genetic material included evauation and analysis of the following parameters: frequency distribution of the antigen (F), χ2 criterion for significance (chi-square), the relative risk ratio (RR), etiologic fraction (EF), preventive fraction (PF). Children of the II group had significantly higher 04 allele HLADRB1, as compared with control group (36.7% vs. 21.1%, χ2 = 10.08; р < 0.01). This finding may suppose a predisposal of these allele carriers to development of tuberculosis. At the same time, the rates of 07 (14.3% vs. 27.5%, χ2 = 7.15, р < 0.01) and 15 (18.4% vs. 28.3%, χ2 = 3.92; р < 0.01) HLA-DRB1 alleles were significantly lower, thus suggesting a protective effect of this allele. 04 allele seems to be a predisposing factor, whereas 07 and *15 alleles are protective for development of tuberculosis in children.A. A. StarshinovaN. V. KornevaI. F. DovgalyukM. V. PavlovaI. E. PavlovaL. N. BubnovaSPb RAACIarticlechildrendiagnosticimmunogenetictuberculosishla-drb1Immunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 17, Iss 3, Pp 275-280 (2015)
institution	DOAJ
collection	DOAJ
language	RU
topic	children diagnostic immunogenetic tuberculosis hla-drb1 Immunologic diseases. Allergy RC581-607
spellingShingle	children diagnostic immunogenetic tuberculosis hla-drb1 Immunologic diseases. Allergy RC581-607 A. A. Starshinova N. V. Korneva I. F. Dovgalyuk M. V. Pavlova I. E. Pavlova L. N. Bubnova INFLUENCE OF HLA-DRB1* ALLELIC SETS ON THE DEVELOPMENT OF TUBERCULOSIS IN CHILDREN
description	According to the WHO data, tuberculosis still represents a serious public health problem worldwide. Deterioration of socio-economic conditions in the population complicates epidemic situation for tuberculosis inRussia, thus leading to increase in acute progressive and complicated forms of tuberculosis in children and, consequently, to worsening structure of its clinical forms. Objectives: to determine associations between certain HLA-DRB1 alleles and risk of tuberculosis development in children. We examined 188 children aged from 3 to 14 years with various manifestations of tuberculous infection. Along with thorough examination of the patients, including multi-spiral CT scans of chest, we undertook genotyping of HLA-DRB1 alleles. Activity of tuberculous infection was determined by a set of immunological tests, i.e., tuberculin skin test, DIASKINTEST® (recombinant allergen of tuberculosis DIASKINTEST®). X ray diagnostics was performed with multi-spiral «Aquilion-32» computed tomograph (Toshiba), according to standard procedures. Molecular genetic typing of HLA-DRB1 alleles was performed by polymerase chain reaction (PCR-SSP), using standard commercial kits PROTRANS Ceclerplate System Protrans HLA-DRB1. The children were divided into two groups: I group, 90 healthy children, II group, 98 children with tuberculosis. A comparisons group consisted of healthy donors (n = 346). Statistical processing of genetic material included evauation and analysis of the following parameters: frequency distribution of the antigen (F), χ2 criterion for significance (chi-square), the relative risk ratio (RR), etiologic fraction (EF), preventive fraction (PF). Children of the II group had significantly higher 04 allele HLADRB1, as compared with control group (36.7% vs. 21.1%, χ2 = 10.08; р < 0.01). This finding may suppose a predisposal of these allele carriers to development of tuberculosis. At the same time, the rates of 07 (14.3% vs. 27.5%, χ2 = 7.15, р < 0.01) and 15 (18.4% vs. 28.3%, χ2 = 3.92; р < 0.01) HLA-DRB1 alleles were significantly lower, thus suggesting a protective effect of this allele. 04 allele seems to be a predisposing factor, whereas 07 and *15 alleles are protective for development of tuberculosis in children.
format	article
author	A. A. Starshinova N. V. Korneva I. F. Dovgalyuk M. V. Pavlova I. E. Pavlova L. N. Bubnova
author_facet	A. A. Starshinova N. V. Korneva I. F. Dovgalyuk M. V. Pavlova I. E. Pavlova L. N. Bubnova
author_sort	A. A. Starshinova
title	INFLUENCE OF HLA-DRB1* ALLELIC SETS ON THE DEVELOPMENT OF TUBERCULOSIS IN CHILDREN
title_short	INFLUENCE OF HLA-DRB1* ALLELIC SETS ON THE DEVELOPMENT OF TUBERCULOSIS IN CHILDREN
title_full	INFLUENCE OF HLA-DRB1* ALLELIC SETS ON THE DEVELOPMENT OF TUBERCULOSIS IN CHILDREN
title_fullStr	INFLUENCE OF HLA-DRB1* ALLELIC SETS ON THE DEVELOPMENT OF TUBERCULOSIS IN CHILDREN
title_full_unstemmed	INFLUENCE OF HLA-DRB1* ALLELIC SETS ON THE DEVELOPMENT OF TUBERCULOSIS IN CHILDREN
title_sort	influence of hla-drb1* allelic sets on the development of tuberculosis in children
publisher	SPb RAACI
publishDate	2015
url	https://doaj.org/article/d85787992cad458ea6f40cc1750e991f
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INFLUENCE OF HLA-DRB1* ALLELIC SETS ON THE DEVELOPMENT OF TUBERCULOSIS IN CHILDREN

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