Structural and physiological effects of chromium uptake in the seagrass Halophila stipulacea

This work aims to provide insight on the effects of chromium (Cr) uptake in seagrasses at environmentally relevant exposure concentrations; information on this issue can further increase the utility of marine angiosperms as indicators of trace metal contamination. Cr uptake kinetics, effects on phys...

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Autores principales:	Paraskevi Malea, Zoi Mylona, Emmanuel Panteris, Theodoros Kevrekidis
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Marine plant Hexavalent chromium Accumulation kinetics Toxicity Bioindicator Biomarker Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/d85d641c479e47d0a5dfd832f13a9d5e
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spelling	oai:doaj.org-article:d85d641c479e47d0a5dfd832f13a9d5e2021-12-01T04:39:28ZStructural and physiological effects of chromium uptake in the seagrass Halophila stipulacea1470-160X10.1016/j.ecolind.2020.107224https://doaj.org/article/d85d641c479e47d0a5dfd832f13a9d5e2021-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X20311638https://doaj.org/toc/1470-160XThis work aims to provide insight on the effects of chromium (Cr) uptake in seagrasses at environmentally relevant exposure concentrations; information on this issue can further increase the utility of marine angiosperms as indicators of trace metal contamination. Cr uptake kinetics, effects on physiological and structural cell traits, and the uptake-effects relationship in Halophila stipulacea leaves incubated in 0.01–10 mg L−1 (0.2–200 μM) of Cr (VI) were examined. An initial uptake was followed by a decrease at 0.01–0.5 mg L−1 (0.2–10 μM); at higher exposures, the fit of uptake data to a Michaelis-Menten-type equation was significant (p < 0.001). The mean tissue concentration over the exposure period, and the highest tissue concentration attained both increased as exposure concentration (Cw) increased from 0.05 to 10 mg L−1 (1 to 200 μM); uptake rate generally increased with Cw. Cytotoxic effects occurred even at 0.01 mg L−1 (0.2 μM). Actin filaments (AFs) and endoplasmic reticulum (ER) were impacted in a dose- and time- dependent pattern, while microtubule integrity was not markedly affected even at the highest exposure. AF disruption in differentiating cells initiated at 0.01 mg L−1 (0.2 μM), while ER disorganization did so at 0.1 mg L−1 (2 μM); regression equations described the relationships of Cw vs. time required for effect onset, and of Cw vs. AF disturbance progress. Elevated levels of hydrogen peroxide (H2O2) were detected on the 2d. In addition, a significant reduction in epidermal cell viability appeared at 5–10 or 10 mg L−1 (100–200 or 200 μM), depending on the leaf age. The lowest tissue concentration associated with effect onset was 31.05 μg g−1 dry wt (2d, 5 mg L−1); at lower exposures, higher tissue concentrations did not coincide with impact, implying that the uptake rate rather than the tissue concentration relates to the effect onset. The above suggest that (a) Cr (VI) may pose a high risk to seagrass meadows, (b) H. stipulacea can act as a bioindicator of ambient Cr (VI) bioavailability, mainly at elevated exposure concentrations, (c) AF and ER disturbances in seagrass leaf cells could be considered as sensitive biomarkers of Cr (VI)-induced stress, (d) regression equations describing the relationships Cw vs. time required for effect onset and Cw vs. effect progress increase the utility of AF and ER impairment as biomarkers, and (e) the time frame of the exposure should be considered in the interpretation of tissue residues. Our findings improve the utility of seagrasses as indicators of trace metal contamination and could be utilized in the overall effort for seagrass meadows conservation.Paraskevi MaleaZoi MylonaEmmanuel PanterisTheodoros KevrekidisElsevierarticleMarine plantHexavalent chromiumAccumulation kineticsToxicityBioindicatorBiomarkerEcologyQH540-549.5ENEcological Indicators, Vol 122, Iss , Pp 107224- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Marine plant Hexavalent chromium Accumulation kinetics Toxicity Bioindicator Biomarker Ecology QH540-549.5
spellingShingle	Marine plant Hexavalent chromium Accumulation kinetics Toxicity Bioindicator Biomarker Ecology QH540-549.5 Paraskevi Malea Zoi Mylona Emmanuel Panteris Theodoros Kevrekidis Structural and physiological effects of chromium uptake in the seagrass Halophila stipulacea
description	This work aims to provide insight on the effects of chromium (Cr) uptake in seagrasses at environmentally relevant exposure concentrations; information on this issue can further increase the utility of marine angiosperms as indicators of trace metal contamination. Cr uptake kinetics, effects on physiological and structural cell traits, and the uptake-effects relationship in Halophila stipulacea leaves incubated in 0.01–10 mg L−1 (0.2–200 μM) of Cr (VI) were examined. An initial uptake was followed by a decrease at 0.01–0.5 mg L−1 (0.2–10 μM); at higher exposures, the fit of uptake data to a Michaelis-Menten-type equation was significant (p < 0.001). The mean tissue concentration over the exposure period, and the highest tissue concentration attained both increased as exposure concentration (Cw) increased from 0.05 to 10 mg L−1 (1 to 200 μM); uptake rate generally increased with Cw. Cytotoxic effects occurred even at 0.01 mg L−1 (0.2 μM). Actin filaments (AFs) and endoplasmic reticulum (ER) were impacted in a dose- and time- dependent pattern, while microtubule integrity was not markedly affected even at the highest exposure. AF disruption in differentiating cells initiated at 0.01 mg L−1 (0.2 μM), while ER disorganization did so at 0.1 mg L−1 (2 μM); regression equations described the relationships of Cw vs. time required for effect onset, and of Cw vs. AF disturbance progress. Elevated levels of hydrogen peroxide (H2O2) were detected on the 2d. In addition, a significant reduction in epidermal cell viability appeared at 5–10 or 10 mg L−1 (100–200 or 200 μM), depending on the leaf age. The lowest tissue concentration associated with effect onset was 31.05 μg g−1 dry wt (2d, 5 mg L−1); at lower exposures, higher tissue concentrations did not coincide with impact, implying that the uptake rate rather than the tissue concentration relates to the effect onset. The above suggest that (a) Cr (VI) may pose a high risk to seagrass meadows, (b) H. stipulacea can act as a bioindicator of ambient Cr (VI) bioavailability, mainly at elevated exposure concentrations, (c) AF and ER disturbances in seagrass leaf cells could be considered as sensitive biomarkers of Cr (VI)-induced stress, (d) regression equations describing the relationships Cw vs. time required for effect onset and Cw vs. effect progress increase the utility of AF and ER impairment as biomarkers, and (e) the time frame of the exposure should be considered in the interpretation of tissue residues. Our findings improve the utility of seagrasses as indicators of trace metal contamination and could be utilized in the overall effort for seagrass meadows conservation.
format	article
author	Paraskevi Malea Zoi Mylona Emmanuel Panteris Theodoros Kevrekidis
author_facet	Paraskevi Malea Zoi Mylona Emmanuel Panteris Theodoros Kevrekidis
author_sort	Paraskevi Malea
title	Structural and physiological effects of chromium uptake in the seagrass Halophila stipulacea
title_short	Structural and physiological effects of chromium uptake in the seagrass Halophila stipulacea
title_full	Structural and physiological effects of chromium uptake in the seagrass Halophila stipulacea
title_fullStr	Structural and physiological effects of chromium uptake in the seagrass Halophila stipulacea
title_full_unstemmed	Structural and physiological effects of chromium uptake in the seagrass Halophila stipulacea
title_sort	structural and physiological effects of chromium uptake in the seagrass halophila stipulacea
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/d85d641c479e47d0a5dfd832f13a9d5e
work_keys_str_mv	AT paraskevimalea structuralandphysiologicaleffectsofchromiumuptakeintheseagrasshalophilastipulacea AT zoimylona structuralandphysiologicaleffectsofchromiumuptakeintheseagrasshalophilastipulacea AT emmanuelpanteris structuralandphysiologicaleffectsofchromiumuptakeintheseagrasshalophilastipulacea AT theodoroskevrekidis structuralandphysiologicaleffectsofchromiumuptakeintheseagrasshalophilastipulacea
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Structural and physiological effects of chromium uptake in the seagrass Halophila stipulacea

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