Formulation and evaluation of Bacillus coagulans-loaded hypromellose mucoadhesive microspheres

Sk Md Athar AlliDepartment of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, IndiaAbstract: Development of a novel delivery system has been attempted to deliver viable probiotic cells into the gut for a prolonged period of time while maintaining high numbers of viable cells wi...

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Autor principal:	Alli SMA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d863611abbce4e0fa17aba8f4d4b01a0
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spelling	oai:doaj.org-article:d863611abbce4e0fa17aba8f4d4b01a02021-12-02T07:37:56ZFormulation and evaluation of Bacillus coagulans-loaded hypromellose mucoadhesive microspheres1176-91141178-2013https://doaj.org/article/d863611abbce4e0fa17aba8f4d4b01a02011-03-01T00:00:00Zhttp://www.dovepress.com/formulation-and-evaluation-of-bacillus-coagulans-loaded-hypromellose-m-a6885https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Sk Md Athar AlliDepartment of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, IndiaAbstract: Development of a novel delivery system has been attempted to deliver viable probiotic cells into the gut for a prolonged period of time while maintaining high numbers of viable cells within the formulation throughout the shelf-life of the product and during the gastrointestinal transit. Core mucoadhesive microspheres of Bacillus coagulans were developed employing several grades of hypromellose, a mucoadhesive polymer, following coacervation and phase separation technique and were subsequently enteric-coated with hypromellose phthalate. Microspheres were evaluated for percent yield; entrapment efficiency; in vitro swelling; surface morphology; particle size, size distribution, and zeta potential; flow property, mucoadhesion property by the ex vivo mucoadhesive strength test and the in vitro wash off test; in vitro release profile and release kinetic; in vivo probiotic activity; and stability. The values for the kinetic constant and regression coefficient of model-dependent approaches and the difference factor ( &fnof;1), the similarity factor (&fnof;2), and the Rescigno index (&xi;1 and &xi; 2) of model independent approaches were determined for comparing in vitro dissolution profiles. Freeze dried B. coagulans cells were successfully formulated as enteric-coated mucoadhesive microspheres with satisfactory physical structure and yield. The viability of B. coagulans was maintained in the simulated gastric conditions and during processing; in simulated intestinal conditions exhibiting mucoadhesion, and controlling and extending the viable cell release following zero-order; and was satisfactorily stable at room temperature. Test results depict statistically significant effects of the hypromellose grade and their concentration on the performance and release profile of formulations.Keywords: probiotics, B. coagulans, mucoadhesive, microspheres, extended-release Alli SMADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 619-629 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Alli SMA Formulation and evaluation of Bacillus coagulans-loaded hypromellose mucoadhesive microspheres
description	Sk Md Athar AlliDepartment of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, IndiaAbstract: Development of a novel delivery system has been attempted to deliver viable probiotic cells into the gut for a prolonged period of time while maintaining high numbers of viable cells within the formulation throughout the shelf-life of the product and during the gastrointestinal transit. Core mucoadhesive microspheres of Bacillus coagulans were developed employing several grades of hypromellose, a mucoadhesive polymer, following coacervation and phase separation technique and were subsequently enteric-coated with hypromellose phthalate. Microspheres were evaluated for percent yield; entrapment efficiency; in vitro swelling; surface morphology; particle size, size distribution, and zeta potential; flow property, mucoadhesion property by the ex vivo mucoadhesive strength test and the in vitro wash off test; in vitro release profile and release kinetic; in vivo probiotic activity; and stability. The values for the kinetic constant and regression coefficient of model-dependent approaches and the difference factor ( &fnof;1), the similarity factor (&fnof;2), and the Rescigno index (&xi;1 and &xi; 2) of model independent approaches were determined for comparing in vitro dissolution profiles. Freeze dried B. coagulans cells were successfully formulated as enteric-coated mucoadhesive microspheres with satisfactory physical structure and yield. The viability of B. coagulans was maintained in the simulated gastric conditions and during processing; in simulated intestinal conditions exhibiting mucoadhesion, and controlling and extending the viable cell release following zero-order; and was satisfactorily stable at room temperature. Test results depict statistically significant effects of the hypromellose grade and their concentration on the performance and release profile of formulations.Keywords: probiotics, B. coagulans, mucoadhesive, microspheres, extended-release
format	article
author	Alli SMA
author_facet	Alli SMA
author_sort	Alli SMA
title	Formulation and evaluation of Bacillus coagulans-loaded hypromellose mucoadhesive microspheres
title_short	Formulation and evaluation of Bacillus coagulans-loaded hypromellose mucoadhesive microspheres
title_full	Formulation and evaluation of Bacillus coagulans-loaded hypromellose mucoadhesive microspheres
title_fullStr	Formulation and evaluation of Bacillus coagulans-loaded hypromellose mucoadhesive microspheres
title_full_unstemmed	Formulation and evaluation of Bacillus coagulans-loaded hypromellose mucoadhesive microspheres
title_sort	formulation and evaluation of bacillus coagulans-loaded hypromellose mucoadhesive microspheres
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/d863611abbce4e0fa17aba8f4d4b01a0
work_keys_str_mv	AT allisma formulationandevaluationofbacilluscoagulansloadedhypromellosemucoadhesivemicrospheres
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Formulation and evaluation of Bacillus coagulans-loaded hypromellose mucoadhesive microspheres

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