Analysis of phagoand antibiotic sensitivity of Enterobacteriaceae bacteria isolated from women of reproductive age

Pelvic inflammatory diseases occupy a special place in the structure of general morbidity, and are polymicrobial in nature with dominance of opportunistic microorganisms, in particular bacteria of the family Enterobacteriaceae. The aim was to study the composition of the vaginal microbiota in women...

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Autores principales:	E. V. Grigorova, N. M. Shabanova, U. M. Nemchenko, E. B. Rakova, I. N. Danusevich
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2016
Materias:	enterobacteriaceae antibiotics bacteriophages inflammatory diseases of the pelvic organs Science Q
Acceso en línea:	https://doaj.org/article/d8776430cbdc49dfb7224583bb1c9fbe
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spelling	oai:doaj.org-article:d8776430cbdc49dfb7224583bb1c9fbe2021-11-23T06:14:36ZAnalysis of phagoand antibiotic sensitivity of Enterobacteriaceae bacteria isolated from women of reproductive age2541-94202587-959610.12737/23414https://doaj.org/article/d8776430cbdc49dfb7224583bb1c9fbe2016-09-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/299https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Pelvic inflammatory diseases occupy a special place in the structure of general morbidity, and are polymicrobial in nature with dominance of opportunistic microorganisms, in particular bacteria of the family Enterobacteriaceae. The aim was to study the composition of the vaginal microbiota in women of reproductive age with pelvic inflammatory diseases, as well as to determine the sensitivity of isolated microorganisms to antibiotics and bacteriophages. The study included 70 women of reproductive age, among them 37 were diagnosed with colpitis and cervicitis, 33 women in the comparison group (women screened for a diagnosis). Isolated microorganisms were identified by abdominoperineal methods, including the disk diffusion method to determine the sensitivity of microorganism cultures of Enterobacteriaceae family to antibiotics, and the method of crosses (evaluation of lytic activity of bacteriophages by the number of crosses) to determine the sensitivity to specific therapeutic bacteriophages. Vaginal biocenosis was characterized by deficit of lactobacilli (< 106 CFU/ml in 100 %), the presence of conditionally pathogenic microflora: bacteria of Enterobacteriaceae family, coccal flora and Candida fungi. From 60.0 to 89.3 % of Enterobacteria strains were resistant to aminoglycosides and quinolones, but also had a low level of sensitivity to therapeutic bacteriophages. The obtained data indicate the reduction of colonization resistance of vaginal mucosa in pelvic inflammatory diseases and specify the need to use medicinal drugs only under medical supervision to prevent clinically significant drug resistance.E. V. GrigorovaN. M. ShabanovaU. M. NemchenkoE. B. RakovaI. N. DanusevichScientific Сentre for Family Health and Human Reproduction Problemsarticleenterobacteriaceaeantibioticsbacteriophagesinflammatory diseases of the pelvic organsScienceQRUActa Biomedica Scientifica, Vol 1, Iss 5, Pp 150-154 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	enterobacteriaceae antibiotics bacteriophages inflammatory diseases of the pelvic organs Science Q
spellingShingle	enterobacteriaceae antibiotics bacteriophages inflammatory diseases of the pelvic organs Science Q E. V. Grigorova N. M. Shabanova U. M. Nemchenko E. B. Rakova I. N. Danusevich Analysis of phagoand antibiotic sensitivity of Enterobacteriaceae bacteria isolated from women of reproductive age
description	Pelvic inflammatory diseases occupy a special place in the structure of general morbidity, and are polymicrobial in nature with dominance of opportunistic microorganisms, in particular bacteria of the family Enterobacteriaceae. The aim was to study the composition of the vaginal microbiota in women of reproductive age with pelvic inflammatory diseases, as well as to determine the sensitivity of isolated microorganisms to antibiotics and bacteriophages. The study included 70 women of reproductive age, among them 37 were diagnosed with colpitis and cervicitis, 33 women in the comparison group (women screened for a diagnosis). Isolated microorganisms were identified by abdominoperineal methods, including the disk diffusion method to determine the sensitivity of microorganism cultures of Enterobacteriaceae family to antibiotics, and the method of crosses (evaluation of lytic activity of bacteriophages by the number of crosses) to determine the sensitivity to specific therapeutic bacteriophages. Vaginal biocenosis was characterized by deficit of lactobacilli (< 106 CFU/ml in 100 %), the presence of conditionally pathogenic microflora: bacteria of Enterobacteriaceae family, coccal flora and Candida fungi. From 60.0 to 89.3 % of Enterobacteria strains were resistant to aminoglycosides and quinolones, but also had a low level of sensitivity to therapeutic bacteriophages. The obtained data indicate the reduction of colonization resistance of vaginal mucosa in pelvic inflammatory diseases and specify the need to use medicinal drugs only under medical supervision to prevent clinically significant drug resistance.
format	article
author	E. V. Grigorova N. M. Shabanova U. M. Nemchenko E. B. Rakova I. N. Danusevich
author_facet	E. V. Grigorova N. M. Shabanova U. M. Nemchenko E. B. Rakova I. N. Danusevich
author_sort	E. V. Grigorova
title	Analysis of phagoand antibiotic sensitivity of Enterobacteriaceae bacteria isolated from women of reproductive age
title_short	Analysis of phagoand antibiotic sensitivity of Enterobacteriaceae bacteria isolated from women of reproductive age
title_full	Analysis of phagoand antibiotic sensitivity of Enterobacteriaceae bacteria isolated from women of reproductive age
title_fullStr	Analysis of phagoand antibiotic sensitivity of Enterobacteriaceae bacteria isolated from women of reproductive age
title_full_unstemmed	Analysis of phagoand antibiotic sensitivity of Enterobacteriaceae bacteria isolated from women of reproductive age
title_sort	analysis of phagoand antibiotic sensitivity of enterobacteriaceae bacteria isolated from women of reproductive age
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2016
url	https://doaj.org/article/d8776430cbdc49dfb7224583bb1c9fbe
work_keys_str_mv	AT evgrigorova analysisofphagoandantibioticsensitivityofenterobacteriaceaebacteriaisolatedfromwomenofreproductiveage AT nmshabanova analysisofphagoandantibioticsensitivityofenterobacteriaceaebacteriaisolatedfromwomenofreproductiveage AT umnemchenko analysisofphagoandantibioticsensitivityofenterobacteriaceaebacteriaisolatedfromwomenofreproductiveage AT ebrakova analysisofphagoandantibioticsensitivityofenterobacteriaceaebacteriaisolatedfromwomenofreproductiveage AT indanusevich analysisofphagoandantibioticsensitivityofenterobacteriaceaebacteriaisolatedfromwomenofreproductiveage
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Analysis of phagoand antibiotic sensitivity of Enterobacteriaceae bacteria isolated from women of reproductive age

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