RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρ0 cells of Saccharomyces cerevisiae

Abstract Background In Saccharomyces cerevisiae, the retrograde signalling pathway is activated in ρ0/− cells, which lack mitochondrial DNA. Within this pathway, the activation of the transcription factor Pdr3 induces transcription of the ATP-binding cassette (ABC) transporter gene, PDR5, and causes...

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Autor principal:	Yoichi Yamada
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Saccharomyces cerevisiae UME6 RPD3 Pleiotropic drug resistance ρ0 cells Retrograde signalling Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d87cdea4782e474e98e40229a308162f
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spelling	oai:doaj.org-article:d87cdea4782e474e98e40229a308162f2021-11-14T12:08:50ZRPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρ0 cells of Saccharomyces cerevisiae10.1186/s12866-021-02373-11471-2180https://doaj.org/article/d87cdea4782e474e98e40229a308162f2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12866-021-02373-1https://doaj.org/toc/1471-2180Abstract Background In Saccharomyces cerevisiae, the retrograde signalling pathway is activated in ρ0/− cells, which lack mitochondrial DNA. Within this pathway, the activation of the transcription factor Pdr3 induces transcription of the ATP-binding cassette (ABC) transporter gene, PDR5, and causes pleiotropic drug resistance (PDR). Although a histone deacetylase, Rpd3, is also required for cycloheximide resistance in ρ0/− cells, it is currently unknown whether Rpd3 and its DNA binding partners, Ume6 and Ash1, are involved in the activation of PDR5 transcription and PDR in ρ0/− cells. This study investigated the roles of RPD3, UME6, and ASH1 in the activation of PDR5 transcription and PDR by retrograde signalling in ρ0 cells. Results ρ0 cells in the rpd3∆ and ume6∆ strains, with the exception of the ash1∆ strain, were sensitive to fluconazole and cycloheximide. The PDR5 mRNA levels in ρ0 cells of the rpd3∆ and ume6∆ strains were significantly reduced compared to the wild-type and ash1∆ strain. Transcriptional expression of PDR5 was reduced in cycloheximide-exposed and unexposed ρ0 cells of the ume6∆ strain; the transcriptional positive response of PDR5 to cycloheximide exposure was also impaired in this strain. Conclusions RPD3 and UME6 are responsible for enhanced PDR5 mRNA levels and PDR by retrograde signalling in ρ0 cells of S. cerevisiae.Yoichi YamadaBMCarticleSaccharomyces cerevisiaeUME6RPD3Pleiotropic drug resistanceρ0 cellsRetrograde signallingMicrobiologyQR1-502ENBMC Microbiology, Vol 21, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Saccharomyces cerevisiae UME6 RPD3 Pleiotropic drug resistance ρ0 cells Retrograde signalling Microbiology QR1-502
spellingShingle	Saccharomyces cerevisiae UME6 RPD3 Pleiotropic drug resistance ρ0 cells Retrograde signalling Microbiology QR1-502 Yoichi Yamada RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρ0 cells of Saccharomyces cerevisiae
description	Abstract Background In Saccharomyces cerevisiae, the retrograde signalling pathway is activated in ρ0/− cells, which lack mitochondrial DNA. Within this pathway, the activation of the transcription factor Pdr3 induces transcription of the ATP-binding cassette (ABC) transporter gene, PDR5, and causes pleiotropic drug resistance (PDR). Although a histone deacetylase, Rpd3, is also required for cycloheximide resistance in ρ0/− cells, it is currently unknown whether Rpd3 and its DNA binding partners, Ume6 and Ash1, are involved in the activation of PDR5 transcription and PDR in ρ0/− cells. This study investigated the roles of RPD3, UME6, and ASH1 in the activation of PDR5 transcription and PDR by retrograde signalling in ρ0 cells. Results ρ0 cells in the rpd3∆ and ume6∆ strains, with the exception of the ash1∆ strain, were sensitive to fluconazole and cycloheximide. The PDR5 mRNA levels in ρ0 cells of the rpd3∆ and ume6∆ strains were significantly reduced compared to the wild-type and ash1∆ strain. Transcriptional expression of PDR5 was reduced in cycloheximide-exposed and unexposed ρ0 cells of the ume6∆ strain; the transcriptional positive response of PDR5 to cycloheximide exposure was also impaired in this strain. Conclusions RPD3 and UME6 are responsible for enhanced PDR5 mRNA levels and PDR by retrograde signalling in ρ0 cells of S. cerevisiae.
format	article
author	Yoichi Yamada
author_facet	Yoichi Yamada
author_sort	Yoichi Yamada
title	RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρ0 cells of Saccharomyces cerevisiae
title_short	RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρ0 cells of Saccharomyces cerevisiae
title_full	RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρ0 cells of Saccharomyces cerevisiae
title_fullStr	RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρ0 cells of Saccharomyces cerevisiae
title_full_unstemmed	RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρ0 cells of Saccharomyces cerevisiae
title_sort	rpd3 and ume6 are involved in the activation of pdr5 transcription and pleiotropic drug resistance in ρ0 cells of saccharomyces cerevisiae
publisher	BMC
publishDate	2021
url	https://doaj.org/article/d87cdea4782e474e98e40229a308162f
work_keys_str_mv	AT yoichiyamada rpd3andume6areinvolvedintheactivationofpdr5transcriptionandpleiotropicdrugresistanceinr0cellsofsaccharomycescerevisiae
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RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρ0 cells of Saccharomyces cerevisiae

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