Chitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its metastasis

Ping Sun,1,2 Wei Huang,1,2 Mingji Jin,1,2 Qiming Wang,1,2 Bo Fan,1,2 Lin Kang,1,2 Zhonggao Gao1,2 1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, 2Department of Pharmaceutics, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical C...

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Autores principales:	Sun P, Huang W, Jin M, Wang Q, Fan B, Kang L, Gao Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Key words: PEGylated chitosan Non-viral vector siRNA delivery Breast carcinoma Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d87e1dfe41d147bda4b2cf350e2d3338
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spelling	oai:doaj.org-article:d87e1dfe41d147bda4b2cf350e2d33382021-12-02T01:01:50ZChitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its metastasis1178-2013https://doaj.org/article/d87e1dfe41d147bda4b2cf350e2d33382016-09-01T00:00:00Zhttps://www.dovepress.com/chitosan-based-nanoparticles-for-survivin-targeted-sirna-delivery-in-b-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ping Sun,1,2 Wei Huang,1,2 Mingji Jin,1,2 Qiming Wang,1,2 Bo Fan,1,2 Lin Kang,1,2 Zhonggao Gao1,2 1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, 2Department of Pharmaceutics, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China Abstract: Nanoparticle-mediated small interfering RNA (siRNA) delivery is a promising therapeutic strategy in various cancers. However, it is difficult to deliver degradative siRNA to tumor tissue, and thus a safe and efficient vector for siRNA delivery is essential for cancer therapy. In this study, poly(ethylene glycol)-modified chitosan (PEG-CS) was synthesized successfully for delivering nucleic acid drug. We deemed that PEGylated CS could improve its solubility by forming a stable siRNA loaded in nanoparticles, and enhancing transfection efficiency of siRNA-loaded CS nanoparticles in cancer cell line. The research results showed that siRNA loaded in PEGylated CS (PEG-CS/siRNA) nanoparticles with smaller particle size had superior structural stability in the physical environment compared to CS nanoparticles. The data of in vitro antitumor activity revealed that 4T1 tumor cell growth was significantly inhibited and cellular uptake of PEG-CS/siRNA nanoparticles in 4T1 cells was dramatically enhanced compared to naked siRNA groups. The results from flow cytometry and confocal laser scanning microscopy showed that PEG-CS/siRNA nanoparticles were more easily taken up than naked siRNA. Importantly, PEG-CS/siRNA nanoparticles significantly reduced the growth of xenograft tumors of 4T1 cells in vivo. It has been demonstrated that the PEG-CS is a safe and efficient vector for siRNA delivery, and it can effectively reduce tumor growth and prevent metastasis. Keywords: PEGylated chitosan, non-viral vector, siRNA delivery, breast anti-tumor therapySun PHuang WJin MWang QFan BKang LGao ZDove Medical PressarticleKey words: PEGylated chitosanNon-viral vectorsiRNA deliveryBreast carcinomaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 4931-4945 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Key words: PEGylated chitosan Non-viral vector siRNA delivery Breast carcinoma Medicine (General) R5-920
spellingShingle	Key words: PEGylated chitosan Non-viral vector siRNA delivery Breast carcinoma Medicine (General) R5-920 Sun P Huang W Jin M Wang Q Fan B Kang L Gao Z Chitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its metastasis
description	Ping Sun,1,2 Wei Huang,1,2 Mingji Jin,1,2 Qiming Wang,1,2 Bo Fan,1,2 Lin Kang,1,2 Zhonggao Gao1,2 1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, 2Department of Pharmaceutics, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China Abstract: Nanoparticle-mediated small interfering RNA (siRNA) delivery is a promising therapeutic strategy in various cancers. However, it is difficult to deliver degradative siRNA to tumor tissue, and thus a safe and efficient vector for siRNA delivery is essential for cancer therapy. In this study, poly(ethylene glycol)-modified chitosan (PEG-CS) was synthesized successfully for delivering nucleic acid drug. We deemed that PEGylated CS could improve its solubility by forming a stable siRNA loaded in nanoparticles, and enhancing transfection efficiency of siRNA-loaded CS nanoparticles in cancer cell line. The research results showed that siRNA loaded in PEGylated CS (PEG-CS/siRNA) nanoparticles with smaller particle size had superior structural stability in the physical environment compared to CS nanoparticles. The data of in vitro antitumor activity revealed that 4T1 tumor cell growth was significantly inhibited and cellular uptake of PEG-CS/siRNA nanoparticles in 4T1 cells was dramatically enhanced compared to naked siRNA groups. The results from flow cytometry and confocal laser scanning microscopy showed that PEG-CS/siRNA nanoparticles were more easily taken up than naked siRNA. Importantly, PEG-CS/siRNA nanoparticles significantly reduced the growth of xenograft tumors of 4T1 cells in vivo. It has been demonstrated that the PEG-CS is a safe and efficient vector for siRNA delivery, and it can effectively reduce tumor growth and prevent metastasis. Keywords: PEGylated chitosan, non-viral vector, siRNA delivery, breast anti-tumor therapy
format	article
author	Sun P Huang W Jin M Wang Q Fan B Kang L Gao Z
author_facet	Sun P Huang W Jin M Wang Q Fan B Kang L Gao Z
author_sort	Sun P
title	Chitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its metastasis
title_short	Chitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its metastasis
title_full	Chitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its metastasis
title_fullStr	Chitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its metastasis
title_full_unstemmed	Chitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its metastasis
title_sort	chitosan-based nanoparticles for survivin targeted sirna delivery in breast tumor therapy and preventing its metastasis
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/d87e1dfe41d147bda4b2cf350e2d3338
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Chitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its metastasis

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