DNA microarray analysis of Staphylococcus aureus from Nigeria and South Africa.

Staphylococcus aureus is an important human pathogen with an arsenal of virulence factors and a propensity to acquire antibiotic resistance genes. The understanding of the global epidemiology of S. aureus through the use of various typing methods is important in the detection and tracking of novel a...

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Autores principales:	Adebayo Osagie Shittu, Tomiwa Adesoji, Edet Ekpenyong Udo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d8819e74eac14294bc12cae67ab8bdf6
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spelling	oai:doaj.org-article:d8819e74eac14294bc12cae67ab8bdf62021-12-02T20:05:00ZDNA microarray analysis of Staphylococcus aureus from Nigeria and South Africa.1932-620310.1371/journal.pone.0237124https://doaj.org/article/d8819e74eac14294bc12cae67ab8bdf62021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0237124https://doaj.org/toc/1932-6203Staphylococcus aureus is an important human pathogen with an arsenal of virulence factors and a propensity to acquire antibiotic resistance genes. The understanding of the global epidemiology of S. aureus through the use of various typing methods is important in the detection and tracking of novel and epidemic clones in countries and regions. However, detailed information on antibiotic resistance and virulence genes of S. aureus, and its population structure is still limited in Africa. In this study, S. aureus isolates collected in South Africa (n = 38) and Nigeria (n = 2) from 2001-2004 were characterized by spa typing and DNA microarray. The combination of these two methods classified the isolates into seven spa types and three clonal complexes (CCs) i.e. t064-CC8 (n = 17), t037-CC8 (n = 8), t1257-CC8 (n = 6), t045-CC5 (n = 5), t951-CC8 (n = 1), t2723-CC88 (n = 1), t6238-CC8 (n = 1), and untypeable-CC8 (n = 1). A high percentage agreement (>95%) and kappa coefficient (>0.60) was largely observed with antibiotic susceptibility testing and DNA microarray, indicating substantial agreement. Some antibiotic and virulence gene markers were associated with specific clones. The detection of the collagen-binding adhesion (cna) gene was unique for t037-CC8-MRSA while the enterotoxin gene cluster (egc) and staphylococcal complement inhibitor (scn) gene were identified with t045-CC5-MRSA. Moreover, the combination of genes encoding enterotoxins (entA, entB, entK, entQ) was noted with most of the CC8 isolates. The t045-CC5-MRSA clone was positive for the mercury resistance (mer) operon. DNA microarray provides information on antibiotic resistance and virulence gene determinants and can be a useful tool to identify gene markers for specific S. aureus clones in Africa.Adebayo Osagie ShittuTomiwa AdesojiEdet Ekpenyong UdoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0237124 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adebayo Osagie Shittu Tomiwa Adesoji Edet Ekpenyong Udo DNA microarray analysis of Staphylococcus aureus from Nigeria and South Africa.
description	Staphylococcus aureus is an important human pathogen with an arsenal of virulence factors and a propensity to acquire antibiotic resistance genes. The understanding of the global epidemiology of S. aureus through the use of various typing methods is important in the detection and tracking of novel and epidemic clones in countries and regions. However, detailed information on antibiotic resistance and virulence genes of S. aureus, and its population structure is still limited in Africa. In this study, S. aureus isolates collected in South Africa (n = 38) and Nigeria (n = 2) from 2001-2004 were characterized by spa typing and DNA microarray. The combination of these two methods classified the isolates into seven spa types and three clonal complexes (CCs) i.e. t064-CC8 (n = 17), t037-CC8 (n = 8), t1257-CC8 (n = 6), t045-CC5 (n = 5), t951-CC8 (n = 1), t2723-CC88 (n = 1), t6238-CC8 (n = 1), and untypeable-CC8 (n = 1). A high percentage agreement (>95%) and kappa coefficient (>0.60) was largely observed with antibiotic susceptibility testing and DNA microarray, indicating substantial agreement. Some antibiotic and virulence gene markers were associated with specific clones. The detection of the collagen-binding adhesion (cna) gene was unique for t037-CC8-MRSA while the enterotoxin gene cluster (egc) and staphylococcal complement inhibitor (scn) gene were identified with t045-CC5-MRSA. Moreover, the combination of genes encoding enterotoxins (entA, entB, entK, entQ) was noted with most of the CC8 isolates. The t045-CC5-MRSA clone was positive for the mercury resistance (mer) operon. DNA microarray provides information on antibiotic resistance and virulence gene determinants and can be a useful tool to identify gene markers for specific S. aureus clones in Africa.
format	article
author	Adebayo Osagie Shittu Tomiwa Adesoji Edet Ekpenyong Udo
author_facet	Adebayo Osagie Shittu Tomiwa Adesoji Edet Ekpenyong Udo
author_sort	Adebayo Osagie Shittu
title	DNA microarray analysis of Staphylococcus aureus from Nigeria and South Africa.
title_short	DNA microarray analysis of Staphylococcus aureus from Nigeria and South Africa.
title_full	DNA microarray analysis of Staphylococcus aureus from Nigeria and South Africa.
title_fullStr	DNA microarray analysis of Staphylococcus aureus from Nigeria and South Africa.
title_full_unstemmed	DNA microarray analysis of Staphylococcus aureus from Nigeria and South Africa.
title_sort	dna microarray analysis of staphylococcus aureus from nigeria and south africa.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/d8819e74eac14294bc12cae67ab8bdf6
work_keys_str_mv	AT adebayoosagieshittu dnamicroarrayanalysisofstaphylococcusaureusfromnigeriaandsouthafrica AT tomiwaadesoji dnamicroarrayanalysisofstaphylococcusaureusfromnigeriaandsouthafrica AT edetekpenyongudo dnamicroarrayanalysisofstaphylococcusaureusfromnigeriaandsouthafrica
_version_	1718375572379271168

DNA microarray analysis of Staphylococcus aureus from Nigeria and South Africa.

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