Identification of multiple functional receptors for tyramine on an insect secretory epithelium

Abstract The biogenic amine tyramine (TA) regulates many aspects of invertebrate physiology and development. Although three TA receptor subtypes have been identified (TAR1-3), specific receptors have not been linked to physiological responses in native tissue. In the Malpighian (renal) tubule of Dro...

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Autores principales:	Haiying Zhang, Edward M. Blumenthal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d8830c5a13804561bb6777cafb68522a
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spelling	oai:doaj.org-article:d8830c5a13804561bb6777cafb68522a2021-12-02T15:04:55ZIdentification of multiple functional receptors for tyramine on an insect secretory epithelium10.1038/s41598-017-00120-z2045-2322https://doaj.org/article/d8830c5a13804561bb6777cafb68522a2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00120-zhttps://doaj.org/toc/2045-2322Abstract The biogenic amine tyramine (TA) regulates many aspects of invertebrate physiology and development. Although three TA receptor subtypes have been identified (TAR1-3), specific receptors have not been linked to physiological responses in native tissue. In the Malpighian (renal) tubule of Drosophila melanogaster, TA activates a transepithelial chloride conductance, resulting in diuresis and depolarization of the transepithelial potential. In the current work, mutation or RNAi-mediated knockdown in the stellate cells of the tubule of TAR2 (tyrR, CG7431) resulted in a dramatic reduction, but not elimination, of the TA-mediated depolarization. Mutation or knockdown of TAR3 (tyrRII, CG16766) had no effect. However, deletion of both genes, or knockdown of TAR3 on a TAR2 mutant background, eliminated the TA responses. Thus while TAR2 is responsible for the majority of the TA sensitivity of the tubule, TAR3 also contributes to the response. Knockdown or mutation of TAR2 also eliminated the response of tubules to the related amine octopamine (OA), indicating that OA can activate TAR2. This finding contrasts to reports that heterologously expressed TAR2 is highly selective for TA over OA. This is the first report of TA receptor function in a native tissue and indicates unexpected complexity in the physiology of the Malpighian tubule.Haiying ZhangEdward M. BlumenthalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haiying Zhang Edward M. Blumenthal Identification of multiple functional receptors for tyramine on an insect secretory epithelium
description	Abstract The biogenic amine tyramine (TA) regulates many aspects of invertebrate physiology and development. Although three TA receptor subtypes have been identified (TAR1-3), specific receptors have not been linked to physiological responses in native tissue. In the Malpighian (renal) tubule of Drosophila melanogaster, TA activates a transepithelial chloride conductance, resulting in diuresis and depolarization of the transepithelial potential. In the current work, mutation or RNAi-mediated knockdown in the stellate cells of the tubule of TAR2 (tyrR, CG7431) resulted in a dramatic reduction, but not elimination, of the TA-mediated depolarization. Mutation or knockdown of TAR3 (tyrRII, CG16766) had no effect. However, deletion of both genes, or knockdown of TAR3 on a TAR2 mutant background, eliminated the TA responses. Thus while TAR2 is responsible for the majority of the TA sensitivity of the tubule, TAR3 also contributes to the response. Knockdown or mutation of TAR2 also eliminated the response of tubules to the related amine octopamine (OA), indicating that OA can activate TAR2. This finding contrasts to reports that heterologously expressed TAR2 is highly selective for TA over OA. This is the first report of TA receptor function in a native tissue and indicates unexpected complexity in the physiology of the Malpighian tubule.
format	article
author	Haiying Zhang Edward M. Blumenthal
author_facet	Haiying Zhang Edward M. Blumenthal
author_sort	Haiying Zhang
title	Identification of multiple functional receptors for tyramine on an insect secretory epithelium
title_short	Identification of multiple functional receptors for tyramine on an insect secretory epithelium
title_full	Identification of multiple functional receptors for tyramine on an insect secretory epithelium
title_fullStr	Identification of multiple functional receptors for tyramine on an insect secretory epithelium
title_full_unstemmed	Identification of multiple functional receptors for tyramine on an insect secretory epithelium
title_sort	identification of multiple functional receptors for tyramine on an insect secretory epithelium
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d8830c5a13804561bb6777cafb68522a
work_keys_str_mv	AT haiyingzhang identificationofmultiplefunctionalreceptorsfortyramineonaninsectsecretoryepithelium AT edwardmblumenthal identificationofmultiplefunctionalreceptorsfortyramineonaninsectsecretoryepithelium
_version_	1718388986265731072

Identification of multiple functional receptors for tyramine on an insect secretory epithelium

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