Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice.

Human rhinovirus is a key viral trigger for asthma exacerbations. To date, murine studies investigating rhinovirus-induced exacerbation of allergic airways disease have employed systemic sensitisation/intranasal challenge with ovalbumin. In this study, we combined human-rhinovirus infection with a c...

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Autores principales:	Jennifer A Phan, Anthony Kicic, Luke J Berry, Lynette B Fernandes, Graeme R Zosky, Peter D Sly, Alexander N Larcombe
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d88529ac46474f719d545ea3ec0a1325
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spelling	oai:doaj.org-article:d88529ac46474f719d545ea3ec0a13252021-11-18T08:28:08ZRhinovirus exacerbates house-dust-mite induced lung disease in adult mice.1932-620310.1371/journal.pone.0092163https://doaj.org/article/d88529ac46474f719d545ea3ec0a13252014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24632596/?tool=EBIhttps://doaj.org/toc/1932-6203Human rhinovirus is a key viral trigger for asthma exacerbations. To date, murine studies investigating rhinovirus-induced exacerbation of allergic airways disease have employed systemic sensitisation/intranasal challenge with ovalbumin. In this study, we combined human-rhinovirus infection with a clinically relevant mouse model of aero-allergen exposure using house-dust-mite in an attempt to more accurately understand the links between human-rhinovirus infection and exacerbations of asthma. Adult BALB/c mice were intranasally exposed to low-dose house-dust-mite (or vehicle) daily for 10 days. On day 9, mice were inoculated with human-rhinovirus-1B (or UV-inactivated human-rhinovirus-1B). Forty-eight hours after inoculation, we assessed bronchoalveolar cellular inflammation, levels of relevant cytokines/serum antibodies, lung function and responsiveness/sensitivity to methacholine. House-dust-mite exposure did not result in a classical TH2-driven response, but was more representative of noneosinophilic asthma. However, there were significant effects of house-dust-mite exposure on most of the parameters measured including increased cellular inflammation (primarily macrophages and neutrophils), increased total IgE and house-dust-mite-specific IgG1 and increased responsiveness/sensitivity to methacholine. There were limited effects of human-rhinovirus-1B infection alone, and the combination of the two insults resulted in additive increases in neutrophil levels and lung parenchymal responses to methacholine (tissue elastance). We conclude that acute rhinovirus infection exacerbates house-dust-mite-induced lung disease in adult mice. The similarity of our results using the naturally occurring allergen house-dust-mite, to previous studies using ovalbumin, suggests that the exacerbation of allergic airways disease by rhinovirus infection could act via multiple or conserved mechanisms.Jennifer A PhanAnthony KicicLuke J BerryLynette B FernandesGraeme R ZoskyPeter D SlyAlexander N LarcombePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92163 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jennifer A Phan Anthony Kicic Luke J Berry Lynette B Fernandes Graeme R Zosky Peter D Sly Alexander N Larcombe Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice.
description	Human rhinovirus is a key viral trigger for asthma exacerbations. To date, murine studies investigating rhinovirus-induced exacerbation of allergic airways disease have employed systemic sensitisation/intranasal challenge with ovalbumin. In this study, we combined human-rhinovirus infection with a clinically relevant mouse model of aero-allergen exposure using house-dust-mite in an attempt to more accurately understand the links between human-rhinovirus infection and exacerbations of asthma. Adult BALB/c mice were intranasally exposed to low-dose house-dust-mite (or vehicle) daily for 10 days. On day 9, mice were inoculated with human-rhinovirus-1B (or UV-inactivated human-rhinovirus-1B). Forty-eight hours after inoculation, we assessed bronchoalveolar cellular inflammation, levels of relevant cytokines/serum antibodies, lung function and responsiveness/sensitivity to methacholine. House-dust-mite exposure did not result in a classical TH2-driven response, but was more representative of noneosinophilic asthma. However, there were significant effects of house-dust-mite exposure on most of the parameters measured including increased cellular inflammation (primarily macrophages and neutrophils), increased total IgE and house-dust-mite-specific IgG1 and increased responsiveness/sensitivity to methacholine. There were limited effects of human-rhinovirus-1B infection alone, and the combination of the two insults resulted in additive increases in neutrophil levels and lung parenchymal responses to methacholine (tissue elastance). We conclude that acute rhinovirus infection exacerbates house-dust-mite-induced lung disease in adult mice. The similarity of our results using the naturally occurring allergen house-dust-mite, to previous studies using ovalbumin, suggests that the exacerbation of allergic airways disease by rhinovirus infection could act via multiple or conserved mechanisms.
format	article
author	Jennifer A Phan Anthony Kicic Luke J Berry Lynette B Fernandes Graeme R Zosky Peter D Sly Alexander N Larcombe
author_facet	Jennifer A Phan Anthony Kicic Luke J Berry Lynette B Fernandes Graeme R Zosky Peter D Sly Alexander N Larcombe
author_sort	Jennifer A Phan
title	Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice.
title_short	Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice.
title_full	Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice.
title_fullStr	Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice.
title_full_unstemmed	Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice.
title_sort	rhinovirus exacerbates house-dust-mite induced lung disease in adult mice.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d88529ac46474f719d545ea3ec0a1325
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Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice.

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