Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography

Ganoderma (lingzhi) is a famous herbal medicine and edible supplement in oriental countries for a long history. In this study, a simple micellar electrokinetic chromatography (MEKC) method was established for the analysis of nucleosides and bases, the major bioactive components in Ganoderma for the...

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Autores principales:	Feiya Sheng, Songsong Wang, Xiao Luo, Jianbo Xiao, Linfeng Hu, Peng Li
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Ganoderma Nucleosides Bases Micellar electrokinetic chromatography Quality assessment Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/d88ae107bd444ff8a694e6b65c4ef8d7
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spelling	oai:doaj.org-article:d88ae107bd444ff8a694e6b65c4ef8d72021-11-28T04:32:05ZSimultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography2213-453010.1016/j.fshw.2021.11.015https://doaj.org/article/d88ae107bd444ff8a694e6b65c4ef8d72022-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2213453021001117https://doaj.org/toc/2213-4530Ganoderma (lingzhi) is a famous herbal medicine and edible supplement in oriental countries for a long history. In this study, a simple micellar electrokinetic chromatography (MEKC) method was established for the analysis of nucleosides and bases, the major bioactive components in Ganoderma for the first time. By optimizing the borate concentration, the sodium dodecyl sulfate (SDS) concentration and the pH value of running buffer, 10 nucleosides and bases achieved an ideal separation. In real sample analysis, the developed method was successfully used to determine the 10 target analytes in 23 batches of Ganoderma samples from different regions. Results indicated that contents of 10 investigated analytes in each sample showed obvious variation. The principal components analysis (PCA) and hierarchical cluster analysis (HCA) analysis classified the samples into three groups, and the HCA tree visualized the relationships which was mainly contributed by geographical partition. The results indicated geographical origin to be an important factor that affect the accumulation of nucleosides and bases in Ganoderma. In summary, this study provides a simple and practical strategy for quality assessment and cultivation reference of Ganoderma.Feiya ShengSongsong WangXiao LuoJianbo XiaoLinfeng HuPeng LiKeAi Communications Co., Ltd.articleGanodermaNucleosidesBasesMicellar electrokinetic chromatographyQuality assessmentNutrition. Foods and food supplyTX341-641ENFood Science and Human Wellness, Vol 11, Iss 2, Pp 263-268 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ganoderma Nucleosides Bases Micellar electrokinetic chromatography Quality assessment Nutrition. Foods and food supply TX341-641
spellingShingle	Ganoderma Nucleosides Bases Micellar electrokinetic chromatography Quality assessment Nutrition. Foods and food supply TX341-641 Feiya Sheng Songsong Wang Xiao Luo Jianbo Xiao Linfeng Hu Peng Li Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography
description	Ganoderma (lingzhi) is a famous herbal medicine and edible supplement in oriental countries for a long history. In this study, a simple micellar electrokinetic chromatography (MEKC) method was established for the analysis of nucleosides and bases, the major bioactive components in Ganoderma for the first time. By optimizing the borate concentration, the sodium dodecyl sulfate (SDS) concentration and the pH value of running buffer, 10 nucleosides and bases achieved an ideal separation. In real sample analysis, the developed method was successfully used to determine the 10 target analytes in 23 batches of Ganoderma samples from different regions. Results indicated that contents of 10 investigated analytes in each sample showed obvious variation. The principal components analysis (PCA) and hierarchical cluster analysis (HCA) analysis classified the samples into three groups, and the HCA tree visualized the relationships which was mainly contributed by geographical partition. The results indicated geographical origin to be an important factor that affect the accumulation of nucleosides and bases in Ganoderma. In summary, this study provides a simple and practical strategy for quality assessment and cultivation reference of Ganoderma.
format	article
author	Feiya Sheng Songsong Wang Xiao Luo Jianbo Xiao Linfeng Hu Peng Li
author_facet	Feiya Sheng Songsong Wang Xiao Luo Jianbo Xiao Linfeng Hu Peng Li
author_sort	Feiya Sheng
title	Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography
title_short	Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography
title_full	Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography
title_fullStr	Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography
title_full_unstemmed	Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography
title_sort	simultaneous determination of ten nucleosides and bases in ganoderma by micellar electrokinetic chromatography
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/d88ae107bd444ff8a694e6b65c4ef8d7
work_keys_str_mv	AT feiyasheng simultaneousdeterminationoftennucleosidesandbasesinganodermabymicellarelectrokineticchromatography AT songsongwang simultaneousdeterminationoftennucleosidesandbasesinganodermabymicellarelectrokineticchromatography AT xiaoluo simultaneousdeterminationoftennucleosidesandbasesinganodermabymicellarelectrokineticchromatography AT jianboxiao simultaneousdeterminationoftennucleosidesandbasesinganodermabymicellarelectrokineticchromatography AT linfenghu simultaneousdeterminationoftennucleosidesandbasesinganodermabymicellarelectrokineticchromatography AT pengli simultaneousdeterminationoftennucleosidesandbasesinganodermabymicellarelectrokineticchromatography
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Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography

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