THE RAPID CULTURE METHOD FOR THE INDICATION OF RABIES VIRUS ANTIGEN IN INFECTED CELL CULTURES

Abstract. The rapid culture method for the indication of antigens of rabies virus has been improved. It allows to detect the antigen of rabies virus within 5 hours from the moment the cell cultures was infected. To achieve this aim it is necessary to use cell culture BHK-F, polyclonal antirabies FIT...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	E. P. Barkova, F. G. Nagieva, V. G. Nikulina, A. N. Lisakov
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2014
Materias:	rabies virus infect cell rapid cell culture method paraformaldehyde Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/d89014376d21430eba1351ce40238d03
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d89014376d21430eba1351ce40238d03
record_format	dspace
spelling	oai:doaj.org-article:d89014376d21430eba1351ce40238d032021-11-22T07:09:46ZTHE RAPID CULTURE METHOD FOR THE INDICATION OF RABIES VIRUS ANTIGEN IN INFECTED CELL CULTURES2220-76192313-739810.15789/2220-7619-2013-4-323-326https://doaj.org/article/d89014376d21430eba1351ce40238d032014-07-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/140https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Abstract. The rapid culture method for the indication of antigens of rabies virus has been improved. It allows to detect the antigen of rabies virus within 5 hours from the moment the cell cultures was infected. To achieve this aim it is necessary to use cell culture BHK-F, polyclonal antirabies FITC labelled immunoglobulin and direct method of membrane immunofluorescense. Applying of twofold freshly filtrated 3% paraformaldehyde as fixing solution allows to detect early virus proteins of rabies virus.E. P. BarkovaF. G. NagievaV. G. NikulinaA. N. LisakovSankt-Peterburg : NIIÈM imeni Pasteraarticlerabies virusinfect cellrapid cell culture methodparaformaldehydeInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 3, Iss 4, Pp 323-326 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	rabies virus infect cell rapid cell culture method paraformaldehyde Infectious and parasitic diseases RC109-216
spellingShingle	rabies virus infect cell rapid cell culture method paraformaldehyde Infectious and parasitic diseases RC109-216 E. P. Barkova F. G. Nagieva V. G. Nikulina A. N. Lisakov THE RAPID CULTURE METHOD FOR THE INDICATION OF RABIES VIRUS ANTIGEN IN INFECTED CELL CULTURES
description	Abstract. The rapid culture method for the indication of antigens of rabies virus has been improved. It allows to detect the antigen of rabies virus within 5 hours from the moment the cell cultures was infected. To achieve this aim it is necessary to use cell culture BHK-F, polyclonal antirabies FITC labelled immunoglobulin and direct method of membrane immunofluorescense. Applying of twofold freshly filtrated 3% paraformaldehyde as fixing solution allows to detect early virus proteins of rabies virus.
format	article
author	E. P. Barkova F. G. Nagieva V. G. Nikulina A. N. Lisakov
author_facet	E. P. Barkova F. G. Nagieva V. G. Nikulina A. N. Lisakov
author_sort	E. P. Barkova
title	THE RAPID CULTURE METHOD FOR THE INDICATION OF RABIES VIRUS ANTIGEN IN INFECTED CELL CULTURES
title_short	THE RAPID CULTURE METHOD FOR THE INDICATION OF RABIES VIRUS ANTIGEN IN INFECTED CELL CULTURES
title_full	THE RAPID CULTURE METHOD FOR THE INDICATION OF RABIES VIRUS ANTIGEN IN INFECTED CELL CULTURES
title_fullStr	THE RAPID CULTURE METHOD FOR THE INDICATION OF RABIES VIRUS ANTIGEN IN INFECTED CELL CULTURES
title_full_unstemmed	THE RAPID CULTURE METHOD FOR THE INDICATION OF RABIES VIRUS ANTIGEN IN INFECTED CELL CULTURES
title_sort	rapid culture method for the indication of rabies virus antigen in infected cell cultures
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2014
url	https://doaj.org/article/d89014376d21430eba1351ce40238d03
work_keys_str_mv	AT epbarkova therapidculturemethodfortheindicationofrabiesvirusantigenininfectedcellcultures AT fgnagieva therapidculturemethodfortheindicationofrabiesvirusantigenininfectedcellcultures AT vgnikulina therapidculturemethodfortheindicationofrabiesvirusantigenininfectedcellcultures AT anlisakov therapidculturemethodfortheindicationofrabiesvirusantigenininfectedcellcultures AT epbarkova rapidculturemethodfortheindicationofrabiesvirusantigenininfectedcellcultures AT fgnagieva rapidculturemethodfortheindicationofrabiesvirusantigenininfectedcellcultures AT vgnikulina rapidculturemethodfortheindicationofrabiesvirusantigenininfectedcellcultures AT anlisakov rapidculturemethodfortheindicationofrabiesvirusantigenininfectedcellcultures
_version_	1718418072806621184

THE RAPID CULTURE METHOD FOR THE INDICATION OF RABIES VIRUS ANTIGEN IN INFECTED CELL CULTURES

Ejemplares similares