Direct molecular detection and genotyping of Borrelia burgdorferi from whole blood of patients with early Lyme disease.

Direct molecular tests in blood for early Lyme disease can be insensitive due to low amount of circulating Borrelia burgdorferi DNA. To address this challenge, we have developed a sensitive strategy to both detect and genotype B. burgdorferi directly from whole blood collected during the initial pat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mark W Eshoo, Christopher C Crowder, Alison W Rebman, Megan A Rounds, Heather E Matthews, John M Picuri, Mark J Soloski, David J Ecker, Steven E Schutzer, John N Aucott
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d89128885f8641bf9f417cc941b43adb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d89128885f8641bf9f417cc941b43adb
record_format	dspace
spelling	oai:doaj.org-article:d89128885f8641bf9f417cc941b43adb2021-11-18T07:19:23ZDirect molecular detection and genotyping of Borrelia burgdorferi from whole blood of patients with early Lyme disease.1932-620310.1371/journal.pone.0036825https://doaj.org/article/d89128885f8641bf9f417cc941b43adb2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22590620/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Direct molecular tests in blood for early Lyme disease can be insensitive due to low amount of circulating Borrelia burgdorferi DNA. To address this challenge, we have developed a sensitive strategy to both detect and genotype B. burgdorferi directly from whole blood collected during the initial patient visit. This strategy improved sensitivity by employing 1.25 mL of whole blood, a novel pre-enrichment of the entire specimen extract for Borrelia DNA prior to a multi-locus PCR and electrospray ionization mass spectrometry detection assay. We evaluated the assay on blood collected at the initial presentation from 21 endemic area patients who had both physician-diagnosed erythema migrans (EM) and positive two-tiered serology either at the initial visit or at a follow-up visit after three weeks of antibiotic therapy. Results of this DNA analysis showed detection of B. burgdorferi in 13 of 21 patients (62%). In most cases the new assay also provided the B. burgdorferi genotype. The combined results of our direct detection assay with initial physician visit serology resulted in the detection of early Lyme disease in 19 of 21 (90%) of patients at the initial visit. In 5 of 21 cases we demonstrate the ability to detect B. burgdorferi in early Lyme disease directly from whole blood specimens prior to seroconversion.Mark W EshooChristopher C CrowderAlison W RebmanMegan A RoundsHeather E MatthewsJohn M PicuriMark J SoloskiDavid J EckerSteven E SchutzerJohn N AucottPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e36825 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mark W Eshoo Christopher C Crowder Alison W Rebman Megan A Rounds Heather E Matthews John M Picuri Mark J Soloski David J Ecker Steven E Schutzer John N Aucott Direct molecular detection and genotyping of Borrelia burgdorferi from whole blood of patients with early Lyme disease.
description	Direct molecular tests in blood for early Lyme disease can be insensitive due to low amount of circulating Borrelia burgdorferi DNA. To address this challenge, we have developed a sensitive strategy to both detect and genotype B. burgdorferi directly from whole blood collected during the initial patient visit. This strategy improved sensitivity by employing 1.25 mL of whole blood, a novel pre-enrichment of the entire specimen extract for Borrelia DNA prior to a multi-locus PCR and electrospray ionization mass spectrometry detection assay. We evaluated the assay on blood collected at the initial presentation from 21 endemic area patients who had both physician-diagnosed erythema migrans (EM) and positive two-tiered serology either at the initial visit or at a follow-up visit after three weeks of antibiotic therapy. Results of this DNA analysis showed detection of B. burgdorferi in 13 of 21 patients (62%). In most cases the new assay also provided the B. burgdorferi genotype. The combined results of our direct detection assay with initial physician visit serology resulted in the detection of early Lyme disease in 19 of 21 (90%) of patients at the initial visit. In 5 of 21 cases we demonstrate the ability to detect B. burgdorferi in early Lyme disease directly from whole blood specimens prior to seroconversion.
format	article
author	Mark W Eshoo Christopher C Crowder Alison W Rebman Megan A Rounds Heather E Matthews John M Picuri Mark J Soloski David J Ecker Steven E Schutzer John N Aucott
author_facet	Mark W Eshoo Christopher C Crowder Alison W Rebman Megan A Rounds Heather E Matthews John M Picuri Mark J Soloski David J Ecker Steven E Schutzer John N Aucott
author_sort	Mark W Eshoo
title	Direct molecular detection and genotyping of Borrelia burgdorferi from whole blood of patients with early Lyme disease.
title_short	Direct molecular detection and genotyping of Borrelia burgdorferi from whole blood of patients with early Lyme disease.
title_full	Direct molecular detection and genotyping of Borrelia burgdorferi from whole blood of patients with early Lyme disease.
title_fullStr	Direct molecular detection and genotyping of Borrelia burgdorferi from whole blood of patients with early Lyme disease.
title_full_unstemmed	Direct molecular detection and genotyping of Borrelia burgdorferi from whole blood of patients with early Lyme disease.
title_sort	direct molecular detection and genotyping of borrelia burgdorferi from whole blood of patients with early lyme disease.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d89128885f8641bf9f417cc941b43adb
work_keys_str_mv	AT markweshoo directmoleculardetectionandgenotypingofborreliaburgdorferifromwholebloodofpatientswithearlylymedisease AT christopherccrowder directmoleculardetectionandgenotypingofborreliaburgdorferifromwholebloodofpatientswithearlylymedisease AT alisonwrebman directmoleculardetectionandgenotypingofborreliaburgdorferifromwholebloodofpatientswithearlylymedisease AT meganarounds directmoleculardetectionandgenotypingofborreliaburgdorferifromwholebloodofpatientswithearlylymedisease AT heatherematthews directmoleculardetectionandgenotypingofborreliaburgdorferifromwholebloodofpatientswithearlylymedisease AT johnmpicuri directmoleculardetectionandgenotypingofborreliaburgdorferifromwholebloodofpatientswithearlylymedisease AT markjsoloski directmoleculardetectionandgenotypingofborreliaburgdorferifromwholebloodofpatientswithearlylymedisease AT davidjecker directmoleculardetectionandgenotypingofborreliaburgdorferifromwholebloodofpatientswithearlylymedisease AT steveneschutzer directmoleculardetectionandgenotypingofborreliaburgdorferifromwholebloodofpatientswithearlylymedisease AT johnnaucott directmoleculardetectionandgenotypingofborreliaburgdorferifromwholebloodofpatientswithearlylymedisease
_version_	1718423630572945408

Direct molecular detection and genotyping of Borrelia burgdorferi from whole blood of patients with early Lyme disease.

Ejemplares similares