Single-cell RNA sequencing reveals a strong connection between Gadd45g upregulation and oncolytic HSV infection in tumor tissue

The oncolytic effect of virotherapy derives from the intrinsic capability of the applied virus in selectively infecting and killing tumor cells. Although oncolytic viruses of various constructions have been shown to efficiently infect and kill tumor cells in vitro, the efficiency of these viruses to...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Divya Ravirala, Guangsheng Pei, Zhongming Zhao, Xiaoliu Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	oncolytic virotherapy oncolytic herpes simplex virus single-cell RNA sequencing tumor microenvironment Gadd45g Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/d89ca230dd9b4cafa2321e204819ba46
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d89ca230dd9b4cafa2321e204819ba46
record_format	dspace
spelling	oai:doaj.org-article:d89ca230dd9b4cafa2321e204819ba462021-11-04T04:33:04ZSingle-cell RNA sequencing reveals a strong connection between Gadd45g upregulation and oncolytic HSV infection in tumor tissue2372-770510.1016/j.omto.2021.10.006https://doaj.org/article/d89ca230dd9b4cafa2321e204819ba462021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2372770521001431https://doaj.org/toc/2372-7705The oncolytic effect of virotherapy derives from the intrinsic capability of the applied virus in selectively infecting and killing tumor cells. Although oncolytic viruses of various constructions have been shown to efficiently infect and kill tumor cells in vitro, the efficiency of these viruses to exert the same effect on tumor cells within tumor tissues in vivo has not been extensively investigated. Here we report our studies using single-cell RNA sequencing to comprehensively analyze the gene expression profile of tumor tissues following herpes simplex virus 2-based oncolytic virotherapy. Our data revealed the extent and cell types within the tumor microenvironment that could be infected by the virus. Moreover, we observed changes in the expression of cellular genes, including antiviral genes, in response to viral infection. One notable gene found to be upregulated significantly in oncolytic virus-infected tumor cells was Gadd45g, which is desirable for optimal virus replication. These results not only help reveal the precise infection status of the oncolytic virus in vivo but also provide insight that may lead to the development of new strategies to further enhance the therapeutic efficacy of oncolytic virotherapy.Divya RaviralaGuangsheng PeiZhongming ZhaoXiaoliu ZhangElsevierarticleoncolytic virotherapyoncolytic herpes simplex virussingle-cell RNA sequencingtumor microenvironmentGadd45gNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENMolecular Therapy: Oncolytics, Vol 23, Iss , Pp 330-341 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oncolytic virotherapy oncolytic herpes simplex virus single-cell RNA sequencing tumor microenvironment Gadd45g Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	oncolytic virotherapy oncolytic herpes simplex virus single-cell RNA sequencing tumor microenvironment Gadd45g Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Divya Ravirala Guangsheng Pei Zhongming Zhao Xiaoliu Zhang Single-cell RNA sequencing reveals a strong connection between Gadd45g upregulation and oncolytic HSV infection in tumor tissue
description	The oncolytic effect of virotherapy derives from the intrinsic capability of the applied virus in selectively infecting and killing tumor cells. Although oncolytic viruses of various constructions have been shown to efficiently infect and kill tumor cells in vitro, the efficiency of these viruses to exert the same effect on tumor cells within tumor tissues in vivo has not been extensively investigated. Here we report our studies using single-cell RNA sequencing to comprehensively analyze the gene expression profile of tumor tissues following herpes simplex virus 2-based oncolytic virotherapy. Our data revealed the extent and cell types within the tumor microenvironment that could be infected by the virus. Moreover, we observed changes in the expression of cellular genes, including antiviral genes, in response to viral infection. One notable gene found to be upregulated significantly in oncolytic virus-infected tumor cells was Gadd45g, which is desirable for optimal virus replication. These results not only help reveal the precise infection status of the oncolytic virus in vivo but also provide insight that may lead to the development of new strategies to further enhance the therapeutic efficacy of oncolytic virotherapy.
format	article
author	Divya Ravirala Guangsheng Pei Zhongming Zhao Xiaoliu Zhang
author_facet	Divya Ravirala Guangsheng Pei Zhongming Zhao Xiaoliu Zhang
author_sort	Divya Ravirala
title	Single-cell RNA sequencing reveals a strong connection between Gadd45g upregulation and oncolytic HSV infection in tumor tissue
title_short	Single-cell RNA sequencing reveals a strong connection between Gadd45g upregulation and oncolytic HSV infection in tumor tissue
title_full	Single-cell RNA sequencing reveals a strong connection between Gadd45g upregulation and oncolytic HSV infection in tumor tissue
title_fullStr	Single-cell RNA sequencing reveals a strong connection between Gadd45g upregulation and oncolytic HSV infection in tumor tissue
title_full_unstemmed	Single-cell RNA sequencing reveals a strong connection between Gadd45g upregulation and oncolytic HSV infection in tumor tissue
title_sort	single-cell rna sequencing reveals a strong connection between gadd45g upregulation and oncolytic hsv infection in tumor tissue
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/d89ca230dd9b4cafa2321e204819ba46
work_keys_str_mv	AT divyaravirala singlecellrnasequencingrevealsastrongconnectionbetweengadd45gupregulationandoncolytichsvinfectionintumortissue AT guangshengpei singlecellrnasequencingrevealsastrongconnectionbetweengadd45gupregulationandoncolytichsvinfectionintumortissue AT zhongmingzhao singlecellrnasequencingrevealsastrongconnectionbetweengadd45gupregulationandoncolytichsvinfectionintumortissue AT xiaoliuzhang singlecellrnasequencingrevealsastrongconnectionbetweengadd45gupregulationandoncolytichsvinfectionintumortissue
_version_	1718445303028252672

Single-cell RNA sequencing reveals a strong connection between Gadd45g upregulation and oncolytic HSV infection in tumor tissue

Ejemplares similares