Development of a protocol for genetic transformation of Malus spp

In this work, a protocol to produce transgenic shoots of Malus X domestica cv Greensleaves was finalized using gene constructs that have been already already used to create parthenocarpic tomato. These gene constructs were: Ino-IaaM and DefH9-IaaM The aim was to obtain a sufficient nº of transgeni...

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Autores principales:	Federico Martinelli, Anna Perrone, Abhaya Dandekar
Formato:	article
Lenguaje:	EN
Publicado:	Firenze University Press 2021
Materias:	Biology (General) QH301-705.5 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/d89d69e25f504a1f82e2b092d5f04691
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spelling	oai:doaj.org-article:d89d69e25f504a1f82e2b092d5f046912021-11-28T20:31:43ZDevelopment of a protocol for genetic transformation of Malus spp 0008-71142165-5391https://doaj.org/article/d89d69e25f504a1f82e2b092d5f046912021-11-01T00:00:00Zhttps://riviste.fupress.net/index.php/caryologia/article/view/1248https://doaj.org/toc/0008-7114https://doaj.org/toc/2165-5391 In this work, a protocol to produce transgenic shoots of Malus X domestica cv Greensleaves was finalized using gene constructs that have been already already used to create parthenocarpic tomato. These gene constructs were: Ino-IaaM and DefH9-IaaM The aim was to obtain a sufficient nº of transgenic shoots that will be in vitro multiplicated, transferred in soil, grafted and tested for parthenocarpy in the next years. We investigated the effects of two modifications of a previous published protocol: 1) the use of co-transformation with an agrobacterium containing “VIP” genes in the gene construct and 2) the use of two different hormone or hormone combinations were tested. The results showed that a greater regeneration of shoots was obtained when the combination of three hormones (BA:NAA:TDZ) was used in co-coltivation step instead of IBA and no co-transformation was performed using VIP gene. For DefH9-IaaM transgene, a percentage of regeneration of 21.04% was achieved for this treatment instead of 8.95% achieved with “IBA treatment” and 4.42% (“Agrobacterium co-transformation treatment”). An increased number of regeneration of shoots was obtained when the combination of three hormones (BA:NAA:TDZ) were used instead of only IBA and no co-transformation was performed using VIP gene. Federico MartinelliAnna PerroneAbhaya DandekarFirenze University PressarticleBiology (General)QH301-705.5CytologyQH573-671ENCaryologia (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5 Cytology QH573-671
spellingShingle	Biology (General) QH301-705.5 Cytology QH573-671 Federico Martinelli Anna Perrone Abhaya Dandekar Development of a protocol for genetic transformation of Malus spp
description	In this work, a protocol to produce transgenic shoots of Malus X domestica cv Greensleaves was finalized using gene constructs that have been already already used to create parthenocarpic tomato. These gene constructs were: Ino-IaaM and DefH9-IaaM The aim was to obtain a sufficient nº of transgenic shoots that will be in vitro multiplicated, transferred in soil, grafted and tested for parthenocarpy in the next years. We investigated the effects of two modifications of a previous published protocol: 1) the use of co-transformation with an agrobacterium containing “VIP” genes in the gene construct and 2) the use of two different hormone or hormone combinations were tested. The results showed that a greater regeneration of shoots was obtained when the combination of three hormones (BA:NAA:TDZ) was used in co-coltivation step instead of IBA and no co-transformation was performed using VIP gene. For DefH9-IaaM transgene, a percentage of regeneration of 21.04% was achieved for this treatment instead of 8.95% achieved with “IBA treatment” and 4.42% (“Agrobacterium co-transformation treatment”). An increased number of regeneration of shoots was obtained when the combination of three hormones (BA:NAA:TDZ) were used instead of only IBA and no co-transformation was performed using VIP gene.
format	article
author	Federico Martinelli Anna Perrone Abhaya Dandekar
author_facet	Federico Martinelli Anna Perrone Abhaya Dandekar
author_sort	Federico Martinelli
title	Development of a protocol for genetic transformation of Malus spp
title_short	Development of a protocol for genetic transformation of Malus spp
title_full	Development of a protocol for genetic transformation of Malus spp
title_fullStr	Development of a protocol for genetic transformation of Malus spp
title_full_unstemmed	Development of a protocol for genetic transformation of Malus spp
title_sort	development of a protocol for genetic transformation of malus spp
publisher	Firenze University Press
publishDate	2021
url	https://doaj.org/article/d89d69e25f504a1f82e2b092d5f04691
work_keys_str_mv	AT federicomartinelli developmentofaprotocolforgenetictransformationofmalusspp AT annaperrone developmentofaprotocolforgenetictransformationofmalusspp AT abhayadandekar developmentofaprotocolforgenetictransformationofmalusspp
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Development of a protocol for genetic transformation of Malus spp

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