Reference gene selection for real‐time quantitative PCR assays in different tissues of Huperzia serrata based on full‐length transcriptome sequencing

Abstract Huperzia serrata (H. serrata) produces various types of effective lycopodium alkaloids, especially Huperzine A (HupA), which is a promising drug for the treatment of Alzheimer's disease. Numerous studies focused on the chemistry, bioactivities, toxicology, and clinical trials of HupA;...

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Autores principales:	Yanping Fu, Fei Niu, Hui Jia, Yanli Wang, Bin Guo, Yahui Wei
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Huperzia serrata Huperzine A reference gene RT‐qPCR Botany QK1-989
Acceso en línea:	https://doaj.org/article/d8a2092f95e0407b849c34597c92ba9a
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spelling	oai:doaj.org-article:d8a2092f95e0407b849c34597c92ba9a2021-11-29T07:25:55ZReference gene selection for real‐time quantitative PCR assays in different tissues of Huperzia serrata based on full‐length transcriptome sequencing2475-445510.1002/pld3.362https://doaj.org/article/d8a2092f95e0407b849c34597c92ba9a2021-11-01T00:00:00Zhttps://doi.org/10.1002/pld3.362https://doaj.org/toc/2475-4455Abstract Huperzia serrata (H. serrata) produces various types of effective lycopodium alkaloids, especially Huperzine A (HupA), which is a promising drug for the treatment of Alzheimer's disease. Numerous studies focused on the chemistry, bioactivities, toxicology, and clinical trials of HupA; however, the public genomic and transcriptomic resources are very limited for H. serrata research, especially for the selection of optimum reference genes. Based on the full‐length transcriptome datasets and previous studies, 10 traditional and three new candidate reference genes were selected in different tissue of H. serrata. Then, two optimal reference genes GAPDHB and HisH2A were confirmed by four analysis methods. In order to further verify the accuracy of the two reference genes, they were used to analyze the expression patterns of four HupA‐biosynthetic genes (lysine decarboxylas, RS‐norcoclaurine 6‐O‐methyltransferase, cytochrome P45072A1, and copper amine oxidase). The data suggested that the expression pattern of HupA‐biosynthetic genes was consistent with them in transcriptome sequencing in different tissue of H. serrata. This study identified that GAPDHB and HisH2A provides the reliable normalization for analyzing the HupA biosynthetic gene expression in different tissues of H. serrata on the transcriptional level.Yanping FuFei NiuHui JiaYanli WangBin GuoYahui WeiWileyarticleHuperzia serrataHuperzine Areference geneRT‐qPCRBotanyQK1-989ENPlant Direct, Vol 5, Iss 11, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Huperzia serrata Huperzine A reference gene RT‐qPCR Botany QK1-989
spellingShingle	Huperzia serrata Huperzine A reference gene RT‐qPCR Botany QK1-989 Yanping Fu Fei Niu Hui Jia Yanli Wang Bin Guo Yahui Wei Reference gene selection for real‐time quantitative PCR assays in different tissues of Huperzia serrata based on full‐length transcriptome sequencing
description	Abstract Huperzia serrata (H. serrata) produces various types of effective lycopodium alkaloids, especially Huperzine A (HupA), which is a promising drug for the treatment of Alzheimer's disease. Numerous studies focused on the chemistry, bioactivities, toxicology, and clinical trials of HupA; however, the public genomic and transcriptomic resources are very limited for H. serrata research, especially for the selection of optimum reference genes. Based on the full‐length transcriptome datasets and previous studies, 10 traditional and three new candidate reference genes were selected in different tissue of H. serrata. Then, two optimal reference genes GAPDHB and HisH2A were confirmed by four analysis methods. In order to further verify the accuracy of the two reference genes, they were used to analyze the expression patterns of four HupA‐biosynthetic genes (lysine decarboxylas, RS‐norcoclaurine 6‐O‐methyltransferase, cytochrome P45072A1, and copper amine oxidase). The data suggested that the expression pattern of HupA‐biosynthetic genes was consistent with them in transcriptome sequencing in different tissue of H. serrata. This study identified that GAPDHB and HisH2A provides the reliable normalization for analyzing the HupA biosynthetic gene expression in different tissues of H. serrata on the transcriptional level.
format	article
author	Yanping Fu Fei Niu Hui Jia Yanli Wang Bin Guo Yahui Wei
author_facet	Yanping Fu Fei Niu Hui Jia Yanli Wang Bin Guo Yahui Wei
author_sort	Yanping Fu
title	Reference gene selection for real‐time quantitative PCR assays in different tissues of Huperzia serrata based on full‐length transcriptome sequencing
title_short	Reference gene selection for real‐time quantitative PCR assays in different tissues of Huperzia serrata based on full‐length transcriptome sequencing
title_full	Reference gene selection for real‐time quantitative PCR assays in different tissues of Huperzia serrata based on full‐length transcriptome sequencing
title_fullStr	Reference gene selection for real‐time quantitative PCR assays in different tissues of Huperzia serrata based on full‐length transcriptome sequencing
title_full_unstemmed	Reference gene selection for real‐time quantitative PCR assays in different tissues of Huperzia serrata based on full‐length transcriptome sequencing
title_sort	reference gene selection for real‐time quantitative pcr assays in different tissues of huperzia serrata based on full‐length transcriptome sequencing
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/d8a2092f95e0407b849c34597c92ba9a
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Reference gene selection for real‐time quantitative PCR assays in different tissues of Huperzia serrata based on full‐length transcriptome sequencing

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