Use of a pollen-based diet to expose the ladybird beetle Propylea japonica to insecticidal proteins.

A rape seed pollen-based diet was developed and found to be suitable for use in a dietary exposure assay for Propylea japonica. Using the diet, we established and validated a dietary exposure assay by using the protease inhibitor E-64 as positive control. Dose-dependent responses were documented for...

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Autores principales:	Xiaojie Zhang, Yunhe Li, Jörg Romeis, Xinming Yin, Kongming Wu, Yufa Peng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d8a8395fb8c84147ab4381d95de46af6
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spelling	oai:doaj.org-article:d8a8395fb8c84147ab4381d95de46af62021-11-18T08:38:43ZUse of a pollen-based diet to expose the ladybird beetle Propylea japonica to insecticidal proteins.1932-620310.1371/journal.pone.0085395https://doaj.org/article/d8a8395fb8c84147ab4381d95de46af62014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24409328/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203A rape seed pollen-based diet was developed and found to be suitable for use in a dietary exposure assay for Propylea japonica. Using the diet, we established and validated a dietary exposure assay by using the protease inhibitor E-64 as positive control. Dose-dependent responses were documented for all observed life-table parameters of P. japonica including survival, pupation and eclosion rates, development time and adult weight. Results suggested that the dietary assay can detect the effects of insecticidal compounds on the survival and development of P. japonica. Using the established dietary assay, we subsequently tested the toxicity of Cry1Ab, Cry1Ac and Cry1F proteins that are expressed by transgenic maize, cotton or rice plants to P. japonica larvae. The diet containing E-64 was included as a positive control. Survival and development of P. japonica larvae were not adversely affected when the diet contained purified Cry1Ab, Cry1Ac, or Cry1F at 500 µg/g diet representing a worst-case exposure scenario. In contrast, P. japonica larvae were adversely affected when the diet contained E-64. The bioactivity and stability of the Cry proteins in the diet and Cry protein uptake by the ladybird larvae were confirmed by bioassay with a Cry-sensitive insect species and by ELISA. The current study describes a suitable experimental system for assessing the potential effects of gut-active insecticidal compounds on ladybird beetle larvae. The experiments with the Cry proteins demonstrate that P. japonica larvae are not sensitive to Cry1Ab, Cry1Ac and Cry1F.Xiaojie ZhangYunhe LiJörg RomeisXinming YinKongming WuYufa PengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e85395 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaojie Zhang Yunhe Li Jörg Romeis Xinming Yin Kongming Wu Yufa Peng Use of a pollen-based diet to expose the ladybird beetle Propylea japonica to insecticidal proteins.
description	A rape seed pollen-based diet was developed and found to be suitable for use in a dietary exposure assay for Propylea japonica. Using the diet, we established and validated a dietary exposure assay by using the protease inhibitor E-64 as positive control. Dose-dependent responses were documented for all observed life-table parameters of P. japonica including survival, pupation and eclosion rates, development time and adult weight. Results suggested that the dietary assay can detect the effects of insecticidal compounds on the survival and development of P. japonica. Using the established dietary assay, we subsequently tested the toxicity of Cry1Ab, Cry1Ac and Cry1F proteins that are expressed by transgenic maize, cotton or rice plants to P. japonica larvae. The diet containing E-64 was included as a positive control. Survival and development of P. japonica larvae were not adversely affected when the diet contained purified Cry1Ab, Cry1Ac, or Cry1F at 500 µg/g diet representing a worst-case exposure scenario. In contrast, P. japonica larvae were adversely affected when the diet contained E-64. The bioactivity and stability of the Cry proteins in the diet and Cry protein uptake by the ladybird larvae were confirmed by bioassay with a Cry-sensitive insect species and by ELISA. The current study describes a suitable experimental system for assessing the potential effects of gut-active insecticidal compounds on ladybird beetle larvae. The experiments with the Cry proteins demonstrate that P. japonica larvae are not sensitive to Cry1Ab, Cry1Ac and Cry1F.
format	article
author	Xiaojie Zhang Yunhe Li Jörg Romeis Xinming Yin Kongming Wu Yufa Peng
author_facet	Xiaojie Zhang Yunhe Li Jörg Romeis Xinming Yin Kongming Wu Yufa Peng
author_sort	Xiaojie Zhang
title	Use of a pollen-based diet to expose the ladybird beetle Propylea japonica to insecticidal proteins.
title_short	Use of a pollen-based diet to expose the ladybird beetle Propylea japonica to insecticidal proteins.
title_full	Use of a pollen-based diet to expose the ladybird beetle Propylea japonica to insecticidal proteins.
title_fullStr	Use of a pollen-based diet to expose the ladybird beetle Propylea japonica to insecticidal proteins.
title_full_unstemmed	Use of a pollen-based diet to expose the ladybird beetle Propylea japonica to insecticidal proteins.
title_sort	use of a pollen-based diet to expose the ladybird beetle propylea japonica to insecticidal proteins.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d8a8395fb8c84147ab4381d95de46af6
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Use of a pollen-based diet to expose the ladybird beetle Propylea japonica to insecticidal proteins.

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