Comparative proteomic analysis of the sweetpotato provides insights into response mechanisms to Fusarium oxysporum f. sp. batatas

Abstract The Fusarium wilt disease caused by Fusarium oxysporum f. sp. batatas (Fob) is one of the devastating diseases of sweetpotato. However, the molecular mechanisms of sweetpotato response to Fob is poorly understood. In the present study, comparative quantitative proteomic analysis was conduct...

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Autores principales:	ShiQiang Lin, ZhiJian Yang, BiFang Huang, ChuYun Bi, XiaoFang Huang, GuoTai Chen, Nuerla Nijiati, XuanYang Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d8a8b6e5a1ef404d9f194a43a9624139
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spelling	oai:doaj.org-article:d8a8b6e5a1ef404d9f194a43a96241392021-12-02T16:08:46ZComparative proteomic analysis of the sweetpotato provides insights into response mechanisms to Fusarium oxysporum f. sp. batatas10.1038/s41598-020-78557-y2045-2322https://doaj.org/article/d8a8b6e5a1ef404d9f194a43a96241392020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78557-yhttps://doaj.org/toc/2045-2322Abstract The Fusarium wilt disease caused by Fusarium oxysporum f. sp. batatas (Fob) is one of the devastating diseases of sweetpotato. However, the molecular mechanisms of sweetpotato response to Fob is poorly understood. In the present study, comparative quantitative proteomic analysis was conducted to investigate the defense mechanisms involved. Two sweetpotato cultivars with differential Fob infection responses were inoculated with Fob spore suspensions and quantitatively analyzed by Tandem Mass Tags (TMT). 2267 proteins were identified and 1897 of them were quantified. There were 817 proteins with quantitative ratios of 1.2-fold change between Fob-inoculated and mock-treated samples. Further, nine differentially expressed proteins were validated by Parallel Reaction Monitoring (PRM). According to Gene Ontology (GO) annotation information, the proteins functioned in molecular metabolism, cellular component formation, and biological processes. Interestingly, the results showed that sweetpotato resistant response to Fob infection included many proteins associated with signaling transduction, plant resistance, chitinase and subtilisin-like protease. The functions and possible roles of those proteins were discussed. The results provides first insight into molecular mechanisms involved in sweetpotato defense responses to Fob.ShiQiang LinZhiJian YangBiFang HuangChuYun BiXiaoFang HuangGuoTai ChenNuerla NijiatiXuanYang ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q ShiQiang Lin ZhiJian Yang BiFang Huang ChuYun Bi XiaoFang Huang GuoTai Chen Nuerla Nijiati XuanYang Chen Comparative proteomic analysis of the sweetpotato provides insights into response mechanisms to Fusarium oxysporum f. sp. batatas
description	Abstract The Fusarium wilt disease caused by Fusarium oxysporum f. sp. batatas (Fob) is one of the devastating diseases of sweetpotato. However, the molecular mechanisms of sweetpotato response to Fob is poorly understood. In the present study, comparative quantitative proteomic analysis was conducted to investigate the defense mechanisms involved. Two sweetpotato cultivars with differential Fob infection responses were inoculated with Fob spore suspensions and quantitatively analyzed by Tandem Mass Tags (TMT). 2267 proteins were identified and 1897 of them were quantified. There were 817 proteins with quantitative ratios of 1.2-fold change between Fob-inoculated and mock-treated samples. Further, nine differentially expressed proteins were validated by Parallel Reaction Monitoring (PRM). According to Gene Ontology (GO) annotation information, the proteins functioned in molecular metabolism, cellular component formation, and biological processes. Interestingly, the results showed that sweetpotato resistant response to Fob infection included many proteins associated with signaling transduction, plant resistance, chitinase and subtilisin-like protease. The functions and possible roles of those proteins were discussed. The results provides first insight into molecular mechanisms involved in sweetpotato defense responses to Fob.
format	article
author	ShiQiang Lin ZhiJian Yang BiFang Huang ChuYun Bi XiaoFang Huang GuoTai Chen Nuerla Nijiati XuanYang Chen
author_facet	ShiQiang Lin ZhiJian Yang BiFang Huang ChuYun Bi XiaoFang Huang GuoTai Chen Nuerla Nijiati XuanYang Chen
author_sort	ShiQiang Lin
title	Comparative proteomic analysis of the sweetpotato provides insights into response mechanisms to Fusarium oxysporum f. sp. batatas
title_short	Comparative proteomic analysis of the sweetpotato provides insights into response mechanisms to Fusarium oxysporum f. sp. batatas
title_full	Comparative proteomic analysis of the sweetpotato provides insights into response mechanisms to Fusarium oxysporum f. sp. batatas
title_fullStr	Comparative proteomic analysis of the sweetpotato provides insights into response mechanisms to Fusarium oxysporum f. sp. batatas
title_full_unstemmed	Comparative proteomic analysis of the sweetpotato provides insights into response mechanisms to Fusarium oxysporum f. sp. batatas
title_sort	comparative proteomic analysis of the sweetpotato provides insights into response mechanisms to fusarium oxysporum f. sp. batatas
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/d8a8b6e5a1ef404d9f194a43a9624139
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Comparative proteomic analysis of the sweetpotato provides insights into response mechanisms to Fusarium oxysporum f. sp. batatas

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