The stability of complement-mediated bactericidal activity in human serum against Salmonella.

The complement cascade includes heat-labile proteins and care is required when handling serum in order to preserve its functional integrity. We have previously used a whole human serum bactericidal assay to show that antibody and an intact complement system are required in blood for killing of invas...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Colette M O'Shaughnessy, Adam F Cunningham, Calman A Maclennan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d8ac1597105d4c1da626b52c7335385e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d8ac1597105d4c1da626b52c7335385e
record_format	dspace
spelling	oai:doaj.org-article:d8ac1597105d4c1da626b52c7335385e2021-11-18T08:09:21ZThe stability of complement-mediated bactericidal activity in human serum against Salmonella.1932-620310.1371/journal.pone.0049147https://doaj.org/article/d8ac1597105d4c1da626b52c7335385e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23145102/?tool=EBIhttps://doaj.org/toc/1932-6203The complement cascade includes heat-labile proteins and care is required when handling serum in order to preserve its functional integrity. We have previously used a whole human serum bactericidal assay to show that antibody and an intact complement system are required in blood for killing of invasive isolates of Salmonella. The aim of the present study was to evaluate the conditions under which human serum can be stored and manipulated while maintaining complement integrity. Serum bactericidal activity against Salmonella was maintained for a minimum of 35 days when stored at 4°C, eight days at 22°C and 54 hours at 37°C. Up to three freeze-thaw cycles had no effect on the persistence of bactericidal activity and hemolytic complement assays confirmed no effect on complement function. Delay in the separation of serum for up to four days from clotted blood stored at 22°C did not affect bactericidal activity. Dilution of serum resulted in an increased rate of loss of bactericidal activity and so serum should be stored undiluted. These findings indicate that the current guidelines concerning manipulation and storage of human serum to preserve complement integrity and function leave a large margin for safety with regards to bactericidal activity against Salmonella. The study provides a scheme for determining the requirements for serum handling in relation to functional activity of complement in other systems.Colette M O'ShaughnessyAdam F CunninghamCalman A MaclennanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e49147 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Colette M O'Shaughnessy Adam F Cunningham Calman A Maclennan The stability of complement-mediated bactericidal activity in human serum against Salmonella.
description	The complement cascade includes heat-labile proteins and care is required when handling serum in order to preserve its functional integrity. We have previously used a whole human serum bactericidal assay to show that antibody and an intact complement system are required in blood for killing of invasive isolates of Salmonella. The aim of the present study was to evaluate the conditions under which human serum can be stored and manipulated while maintaining complement integrity. Serum bactericidal activity against Salmonella was maintained for a minimum of 35 days when stored at 4°C, eight days at 22°C and 54 hours at 37°C. Up to three freeze-thaw cycles had no effect on the persistence of bactericidal activity and hemolytic complement assays confirmed no effect on complement function. Delay in the separation of serum for up to four days from clotted blood stored at 22°C did not affect bactericidal activity. Dilution of serum resulted in an increased rate of loss of bactericidal activity and so serum should be stored undiluted. These findings indicate that the current guidelines concerning manipulation and storage of human serum to preserve complement integrity and function leave a large margin for safety with regards to bactericidal activity against Salmonella. The study provides a scheme for determining the requirements for serum handling in relation to functional activity of complement in other systems.
format	article
author	Colette M O'Shaughnessy Adam F Cunningham Calman A Maclennan
author_facet	Colette M O'Shaughnessy Adam F Cunningham Calman A Maclennan
author_sort	Colette M O'Shaughnessy
title	The stability of complement-mediated bactericidal activity in human serum against Salmonella.
title_short	The stability of complement-mediated bactericidal activity in human serum against Salmonella.
title_full	The stability of complement-mediated bactericidal activity in human serum against Salmonella.
title_fullStr	The stability of complement-mediated bactericidal activity in human serum against Salmonella.
title_full_unstemmed	The stability of complement-mediated bactericidal activity in human serum against Salmonella.
title_sort	stability of complement-mediated bactericidal activity in human serum against salmonella.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d8ac1597105d4c1da626b52c7335385e
work_keys_str_mv	AT colettemoshaughnessy thestabilityofcomplementmediatedbactericidalactivityinhumanserumagainstsalmonella AT adamfcunningham thestabilityofcomplementmediatedbactericidalactivityinhumanserumagainstsalmonella AT calmanamaclennan thestabilityofcomplementmediatedbactericidalactivityinhumanserumagainstsalmonella AT colettemoshaughnessy stabilityofcomplementmediatedbactericidalactivityinhumanserumagainstsalmonella AT adamfcunningham stabilityofcomplementmediatedbactericidalactivityinhumanserumagainstsalmonella AT calmanamaclennan stabilityofcomplementmediatedbactericidalactivityinhumanserumagainstsalmonella
_version_	1718422139182252032

The stability of complement-mediated bactericidal activity in human serum against Salmonella.

Ejemplares similares