Combinatorial ECM Arrays Identify Cooperative Roles for Matricellular Proteins in Enhancing the Generation of TH+ Neurons From Human Pluripotent Cells

The development of efficient cell culture strategies for the generation of dopaminergic neurons is an important goal for transplantation-based approaches to treat Parkinson’s disease. To identify extracellular matrix molecules that enhance differentiation and might be used in these cell cultures we...

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Autores principales:	Maqsood Ahmed, Matthew J. S. Owens, Enrique M. Toledo, Ernest Arenas, Mark Bradley, Charles ffrench-Constant
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	extracellular matrix neural stem cells tyrosine hydroxylase matricellular proteins dopaminergic (DA) neuron array Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d8b2ce2e332846668d6af68faa4ae1e6
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spelling	oai:doaj.org-article:d8b2ce2e332846668d6af68faa4ae1e62021-12-02T00:30:50ZCombinatorial ECM Arrays Identify Cooperative Roles for Matricellular Proteins in Enhancing the Generation of TH+ Neurons From Human Pluripotent Cells2296-634X10.3389/fcell.2021.755406https://doaj.org/article/d8b2ce2e332846668d6af68faa4ae1e62021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.755406/fullhttps://doaj.org/toc/2296-634XThe development of efficient cell culture strategies for the generation of dopaminergic neurons is an important goal for transplantation-based approaches to treat Parkinson’s disease. To identify extracellular matrix molecules that enhance differentiation and might be used in these cell cultures we have used micro-contact printed arrays on glass slides presenting 190 combinations of 19 extracellular matrix molecules selected on the basis of their expression during embryonic development of the ventral midbrain. Using long-term neuroepithelial stem cells (Lt-NES), this approach identified a number of matricellular proteins that enhanced differentiation, with the combination of Sparc, Sparc-like (Sparc-l1) and Nell2 increasing the number of tyrosine hydroxylase+ neurons derived from Lt-NES cells and, critically for further translation, human pluripotent stem cells.Maqsood AhmedMatthew J. S. OwensEnrique M. ToledoErnest ArenasMark BradleyCharles ffrench-ConstantCharles ffrench-ConstantFrontiers Media S.A.articleextracellular matrixneural stem cellstyrosine hydroxylasematricellular proteinsdopaminergic (DA) neuronarrayBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	extracellular matrix neural stem cells tyrosine hydroxylase matricellular proteins dopaminergic (DA) neuron array Biology (General) QH301-705.5
spellingShingle	extracellular matrix neural stem cells tyrosine hydroxylase matricellular proteins dopaminergic (DA) neuron array Biology (General) QH301-705.5 Maqsood Ahmed Matthew J. S. Owens Enrique M. Toledo Ernest Arenas Mark Bradley Charles ffrench-Constant Charles ffrench-Constant Combinatorial ECM Arrays Identify Cooperative Roles for Matricellular Proteins in Enhancing the Generation of TH+ Neurons From Human Pluripotent Cells
description	The development of efficient cell culture strategies for the generation of dopaminergic neurons is an important goal for transplantation-based approaches to treat Parkinson’s disease. To identify extracellular matrix molecules that enhance differentiation and might be used in these cell cultures we have used micro-contact printed arrays on glass slides presenting 190 combinations of 19 extracellular matrix molecules selected on the basis of their expression during embryonic development of the ventral midbrain. Using long-term neuroepithelial stem cells (Lt-NES), this approach identified a number of matricellular proteins that enhanced differentiation, with the combination of Sparc, Sparc-like (Sparc-l1) and Nell2 increasing the number of tyrosine hydroxylase+ neurons derived from Lt-NES cells and, critically for further translation, human pluripotent stem cells.
format	article
author	Maqsood Ahmed Matthew J. S. Owens Enrique M. Toledo Ernest Arenas Mark Bradley Charles ffrench-Constant Charles ffrench-Constant
author_facet	Maqsood Ahmed Matthew J. S. Owens Enrique M. Toledo Ernest Arenas Mark Bradley Charles ffrench-Constant Charles ffrench-Constant
author_sort	Maqsood Ahmed
title	Combinatorial ECM Arrays Identify Cooperative Roles for Matricellular Proteins in Enhancing the Generation of TH+ Neurons From Human Pluripotent Cells
title_short	Combinatorial ECM Arrays Identify Cooperative Roles for Matricellular Proteins in Enhancing the Generation of TH+ Neurons From Human Pluripotent Cells
title_full	Combinatorial ECM Arrays Identify Cooperative Roles for Matricellular Proteins in Enhancing the Generation of TH+ Neurons From Human Pluripotent Cells
title_fullStr	Combinatorial ECM Arrays Identify Cooperative Roles for Matricellular Proteins in Enhancing the Generation of TH+ Neurons From Human Pluripotent Cells
title_full_unstemmed	Combinatorial ECM Arrays Identify Cooperative Roles for Matricellular Proteins in Enhancing the Generation of TH+ Neurons From Human Pluripotent Cells
title_sort	combinatorial ecm arrays identify cooperative roles for matricellular proteins in enhancing the generation of th+ neurons from human pluripotent cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d8b2ce2e332846668d6af68faa4ae1e6
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Combinatorial ECM Arrays Identify Cooperative Roles for Matricellular Proteins in Enhancing the Generation of TH+ Neurons From Human Pluripotent Cells

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