Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA

The diversity of bacteriophages is likely unparalleled in the biome due to the immense variety of hosts and the multitude of viruses that infect them. Recent efforts have led to description at the genomic level of numerous bacteriophages that infect the Actinobacteria, but relatively little is known...

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Autores principales: Jackson Rapala, Brenda Miller, Maximiliano Garcia, Megan Dolan, Matthew Bockman, Mats Hansson, Daniel A. Russell, Rebecca A. Garlena, Steven G. Cresawn, Alexander B. Westbye, J. Thomas Beatty, Richard M. Alvey, David W. Bollivar
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spelling oai:doaj.org-article:d8b572e8fe9f431fa368c1c2da5bf29b2021-11-25T06:19:36ZGenomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA1932-6203https://doaj.org/article/d8b572e8fe9f431fa368c1c2da5bf29b2021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601537/?tool=EBIhttps://doaj.org/toc/1932-6203The diversity of bacteriophages is likely unparalleled in the biome due to the immense variety of hosts and the multitude of viruses that infect them. Recent efforts have led to description at the genomic level of numerous bacteriophages that infect the Actinobacteria, but relatively little is known about those infecting other prokaryotic phyla, such as the purple non-sulfur photosynthetic α-proteobacterium Rhodobacter capsulatus. This species is a common inhabitant of freshwater ecosystems and has been an important model system for the study of photosynthesis. Additionally, it is notable for its utilization of a unique form of horizontal gene transfer via a bacteriophage-like element known as the gene transfer agent (RcGTA). Only three bacteriophages of R. capsulatus had been sequenced prior to this report. Isolation and characterization at the genomic level of 26 new bacteriophages infecting this host advances the understanding of bacteriophage diversity and the origins of RcGTA. These newly discovered isolates can be grouped along with three that were previously sequenced to form six clusters with four remaining as single representatives. These bacteriophages share genes with RcGTA that seem to be related to host recognition. One isolate was found to cause lysis of a marine bacterium when exposed to high-titer lysate. Although some clusters are more highly represented in the sequenced genomes, it is evident that many more bacteriophage types that infect R. capsulatus are likely to be found in the future.Jackson RapalaBrenda MillerMaximiliano GarciaMegan DolanMatthew BockmanMats HanssonDaniel A. RussellRebecca A. GarlenaSteven G. CresawnAlexander B. WestbyeJ. Thomas BeattyRichard M. AlveyDavid W. BollivarPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Jackson Rapala
Brenda Miller
Maximiliano Garcia
Megan Dolan
Matthew Bockman
Mats Hansson
Daniel A. Russell
Rebecca A. Garlena
Steven G. Cresawn
Alexander B. Westbye
J. Thomas Beatty
Richard M. Alvey
David W. Bollivar
Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA
description The diversity of bacteriophages is likely unparalleled in the biome due to the immense variety of hosts and the multitude of viruses that infect them. Recent efforts have led to description at the genomic level of numerous bacteriophages that infect the Actinobacteria, but relatively little is known about those infecting other prokaryotic phyla, such as the purple non-sulfur photosynthetic α-proteobacterium Rhodobacter capsulatus. This species is a common inhabitant of freshwater ecosystems and has been an important model system for the study of photosynthesis. Additionally, it is notable for its utilization of a unique form of horizontal gene transfer via a bacteriophage-like element known as the gene transfer agent (RcGTA). Only three bacteriophages of R. capsulatus had been sequenced prior to this report. Isolation and characterization at the genomic level of 26 new bacteriophages infecting this host advances the understanding of bacteriophage diversity and the origins of RcGTA. These newly discovered isolates can be grouped along with three that were previously sequenced to form six clusters with four remaining as single representatives. These bacteriophages share genes with RcGTA that seem to be related to host recognition. One isolate was found to cause lysis of a marine bacterium when exposed to high-titer lysate. Although some clusters are more highly represented in the sequenced genomes, it is evident that many more bacteriophage types that infect R. capsulatus are likely to be found in the future.
format article
author Jackson Rapala
Brenda Miller
Maximiliano Garcia
Megan Dolan
Matthew Bockman
Mats Hansson
Daniel A. Russell
Rebecca A. Garlena
Steven G. Cresawn
Alexander B. Westbye
J. Thomas Beatty
Richard M. Alvey
David W. Bollivar
author_facet Jackson Rapala
Brenda Miller
Maximiliano Garcia
Megan Dolan
Matthew Bockman
Mats Hansson
Daniel A. Russell
Rebecca A. Garlena
Steven G. Cresawn
Alexander B. Westbye
J. Thomas Beatty
Richard M. Alvey
David W. Bollivar
author_sort Jackson Rapala
title Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA
title_short Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA
title_full Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA
title_fullStr Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA
title_full_unstemmed Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA
title_sort genomic diversity of bacteriophages infecting rhodobacter capsulatus and their relatedness to its gene transfer agent rcgta
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/d8b572e8fe9f431fa368c1c2da5bf29b
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