Tempol differently affects cellular redox changes and antioxidant enzymes in various lung-related cells

Abstract Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl) is a potential redox agent in cells. The present study investigated changes in cellular reactive oxygen species (ROS) and glutathione (GSH) levels and in antioxidant enzymes, in Tempol-treated Calu-6 and A549 lung cancer cells, normal...

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Autor principal:	Woo Hyun Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d8c46cc274cb45c498ea690ce243b174
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spelling	oai:doaj.org-article:d8c46cc274cb45c498ea690ce243b1742021-12-02T17:55:13ZTempol differently affects cellular redox changes and antioxidant enzymes in various lung-related cells10.1038/s41598-021-94340-z2045-2322https://doaj.org/article/d8c46cc274cb45c498ea690ce243b1742021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94340-zhttps://doaj.org/toc/2045-2322Abstract Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl) is a potential redox agent in cells. The present study investigated changes in cellular reactive oxygen species (ROS) and glutathione (GSH) levels and in antioxidant enzymes, in Tempol-treated Calu-6 and A549 lung cancer cells, normal lung WI-38 VA-13 cells, and primary pulmonary fibroblasts. Results demonstrated that Tempol (0.5–4 mM) either increased or decreased general ROS levels in lung cancer and normal cells at 48 h and specifically increased O2 •− levels in these cells. In addition, Tempol differentially altered the expression and activity of antioxidant enzymes such as superoxide dismutase, catalase, and thioredoxin reductase1 (TrxR1) in A549, Calu-6, and WI-38 VA-13 cells. In particular, Tempol treatment increased TrxR1 protein levels in these cells. Tempol at 1 mM inhibited the growth of lung cancer and normal cells by about 50% at 48 h but also significantly induced cell death, as evidenced by annexin V-positive cells. Furthermore, down-regulation of TrxR1 by siRNA had some effect on ROS levels as well as cell growth inhibition and death in Tempol-treated or -untreated lung cells. In addition, some doses of Tempol significantly increased the numbers of GSH-depleted cells in both cancer cells and normal cells at 48 h. In conclusion, Tempol differentially increased or decreased levels of ROS and various antioxidant enzymes in lung cancer and normal cells, and induced growth inhibition and death in all lung cells along with an increase in O2 •− levels and GSH depletion.Woo Hyun ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Woo Hyun Park Tempol differently affects cellular redox changes and antioxidant enzymes in various lung-related cells
description	Abstract Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl) is a potential redox agent in cells. The present study investigated changes in cellular reactive oxygen species (ROS) and glutathione (GSH) levels and in antioxidant enzymes, in Tempol-treated Calu-6 and A549 lung cancer cells, normal lung WI-38 VA-13 cells, and primary pulmonary fibroblasts. Results demonstrated that Tempol (0.5–4 mM) either increased or decreased general ROS levels in lung cancer and normal cells at 48 h and specifically increased O2 •− levels in these cells. In addition, Tempol differentially altered the expression and activity of antioxidant enzymes such as superoxide dismutase, catalase, and thioredoxin reductase1 (TrxR1) in A549, Calu-6, and WI-38 VA-13 cells. In particular, Tempol treatment increased TrxR1 protein levels in these cells. Tempol at 1 mM inhibited the growth of lung cancer and normal cells by about 50% at 48 h but also significantly induced cell death, as evidenced by annexin V-positive cells. Furthermore, down-regulation of TrxR1 by siRNA had some effect on ROS levels as well as cell growth inhibition and death in Tempol-treated or -untreated lung cells. In addition, some doses of Tempol significantly increased the numbers of GSH-depleted cells in both cancer cells and normal cells at 48 h. In conclusion, Tempol differentially increased or decreased levels of ROS and various antioxidant enzymes in lung cancer and normal cells, and induced growth inhibition and death in all lung cells along with an increase in O2 •− levels and GSH depletion.
format	article
author	Woo Hyun Park
author_facet	Woo Hyun Park
author_sort	Woo Hyun Park
title	Tempol differently affects cellular redox changes and antioxidant enzymes in various lung-related cells
title_short	Tempol differently affects cellular redox changes and antioxidant enzymes in various lung-related cells
title_full	Tempol differently affects cellular redox changes and antioxidant enzymes in various lung-related cells
title_fullStr	Tempol differently affects cellular redox changes and antioxidant enzymes in various lung-related cells
title_full_unstemmed	Tempol differently affects cellular redox changes and antioxidant enzymes in various lung-related cells
title_sort	tempol differently affects cellular redox changes and antioxidant enzymes in various lung-related cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d8c46cc274cb45c498ea690ce243b174
work_keys_str_mv	AT woohyunpark tempoldifferentlyaffectscellularredoxchangesandantioxidantenzymesinvariouslungrelatedcells
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Tempol differently affects cellular redox changes and antioxidant enzymes in various lung-related cells

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