A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties

Kena Ma,1,2,* Lingling Gong,1,* Xinjie Cai,1,2 Pin Huang,1 Jing Cai,1 Dan Huang,1 Tao Jiang1,2 1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, 2Department of P...

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Autores principales: Ma K, Gong L, Cai X, Huang P, Cai J, Huang D, Jiang T
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Lenguaje:EN
Publicado: Dove Medical Press 2017
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EPD
Acceso en línea:https://doaj.org/article/d8c6548ad6fa4126b9530c84ac8f2600
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spelling oai:doaj.org-article:d8c6548ad6fa4126b9530c84ac8f26002021-12-02T02:31:35ZA green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties1178-2013https://doaj.org/article/d8c6548ad6fa4126b9530c84ac8f26002017-05-01T00:00:00Zhttps://www.dovepress.com/a-green-single-step-procedure-to-synthesize-ag-containing-nanocomposit-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Kena Ma,1,2,* Lingling Gong,1,* Xinjie Cai,1,2 Pin Huang,1 Jing Cai,1 Dan Huang,1 Tao Jiang1,2 1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, 2Department of Prosthodontics, Hospital of Stomatology, Wuhan University, Wuhan, People’s Republic of China*These authors contributed equally to this workAbstract: Implant-associated infections still pose a serious threat leading to several complications. This study reported an environmentally benign Ag-containing nanocomposite coating with efficient antibacterial property fabricated on the metal implant via electrophoretic deposition (EPD). In such coatings, Ag2O/AgCl mixed with chitosan/gelatin (CS/G) polymers work together to exert the antibacterial property which could act as an alternative to traditional Ag nanoparticles. Scanning electron microscopy images showed the shuttle fiber-like morphology distributed lamellarly and some nanoparticles carved uniformly into the cross section. Transmission electron microscopy results revealed a core–shell-like structure of the released nanoparticles in experimental groups. The Ag-containing coatings exhibited strong antibacterial properties against Staphylococcus aureus strains and Escherichia coli strains. Meanwhile, the CCK-8 tests showed that after assembling with chitosan and gelatin polymers, the cytotoxicity of Ag was largely decreased. In addition, such coatings also exhibited strong bond strength with metal substrates and good degradable properties. Therefore, such Ag-containing CS/G coatings fabricated via EPD may be a promising candidate to be administrated in controlling the implant-associated infections.Keywords: infection, green chemistry, chitosan, gelatin, EPDMa KGong LCai XHuang PCai JHuang DJiang TDove Medical Pressarticlenanocomposite coatingssilverchitosangelatinEPDMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3665-3679 (2017)
institution DOAJ
collection DOAJ
language EN
topic nanocomposite coatings
silver
chitosan
gelatin
EPD
Medicine (General)
R5-920
spellingShingle nanocomposite coatings
silver
chitosan
gelatin
EPD
Medicine (General)
R5-920
Ma K
Gong L
Cai X
Huang P
Cai J
Huang D
Jiang T
A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties
description Kena Ma,1,2,* Lingling Gong,1,* Xinjie Cai,1,2 Pin Huang,1 Jing Cai,1 Dan Huang,1 Tao Jiang1,2 1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, 2Department of Prosthodontics, Hospital of Stomatology, Wuhan University, Wuhan, People’s Republic of China*These authors contributed equally to this workAbstract: Implant-associated infections still pose a serious threat leading to several complications. This study reported an environmentally benign Ag-containing nanocomposite coating with efficient antibacterial property fabricated on the metal implant via electrophoretic deposition (EPD). In such coatings, Ag2O/AgCl mixed with chitosan/gelatin (CS/G) polymers work together to exert the antibacterial property which could act as an alternative to traditional Ag nanoparticles. Scanning electron microscopy images showed the shuttle fiber-like morphology distributed lamellarly and some nanoparticles carved uniformly into the cross section. Transmission electron microscopy results revealed a core–shell-like structure of the released nanoparticles in experimental groups. The Ag-containing coatings exhibited strong antibacterial properties against Staphylococcus aureus strains and Escherichia coli strains. Meanwhile, the CCK-8 tests showed that after assembling with chitosan and gelatin polymers, the cytotoxicity of Ag was largely decreased. In addition, such coatings also exhibited strong bond strength with metal substrates and good degradable properties. Therefore, such Ag-containing CS/G coatings fabricated via EPD may be a promising candidate to be administrated in controlling the implant-associated infections.Keywords: infection, green chemistry, chitosan, gelatin, EPD
format article
author Ma K
Gong L
Cai X
Huang P
Cai J
Huang D
Jiang T
author_facet Ma K
Gong L
Cai X
Huang P
Cai J
Huang D
Jiang T
author_sort Ma K
title A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties
title_short A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties
title_full A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties
title_fullStr A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties
title_full_unstemmed A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties
title_sort green single-step procedure to synthesize ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties
publisher Dove Medical Press
publishDate 2017
url https://doaj.org/article/d8c6548ad6fa4126b9530c84ac8f2600
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