The acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than Klason and thioglycolic acid methods.

We compared the amount of lignin as determined by the three most traditional methods for lignin measurement in three tissues (sugarcane bagasse, soybean roots and soybean seed coat) contrasting for lignin amount and composition. Although all methods presented high reproducibility, major inconsistenc...

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Autores principales:	Flavia Carolina Moreira-Vilar, Rita de Cássia Siqueira-Soares, Aline Finger-Teixeira, Dyoni Matias de Oliveira, Ana Paula Ferro, George Jackson da Rocha, Maria de Lourdes L Ferrarese, Wanderley Dantas dos Santos, Osvaldo Ferrarese-Filho
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d8c889c93d8042f3aa125da74cc0a800
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spelling	oai:doaj.org-article:d8c889c93d8042f3aa125da74cc0a8002021-11-25T05:56:17ZThe acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than Klason and thioglycolic acid methods.1932-620310.1371/journal.pone.0110000https://doaj.org/article/d8c889c93d8042f3aa125da74cc0a8002014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0110000https://doaj.org/toc/1932-6203We compared the amount of lignin as determined by the three most traditional methods for lignin measurement in three tissues (sugarcane bagasse, soybean roots and soybean seed coat) contrasting for lignin amount and composition. Although all methods presented high reproducibility, major inconsistencies among them were found. The amount of lignin determined by thioglycolic acid method was severely lower than that provided by the other methods (up to 95%) in all tissues analyzed. Klason method was quite similar to acetyl bromide in tissues containing higher amounts of lignin, but presented lower recovery of lignin in the less lignified tissue. To investigate the causes of the inconsistencies observed, we determined the monomer composition of all plant materials, but found no correlation. We found that the low recovery of lignin presented by the thioglycolic acid method were due losses of lignin in the residues disposed throughout the procedures. The production of furfurals by acetyl bromide method does not explain the differences observed. The acetyl bromide method is the simplest and fastest among the methods evaluated presenting similar or best recovery of lignin in all the tissues assessed.Flavia Carolina Moreira-VilarRita de Cássia Siqueira-SoaresAline Finger-TeixeiraDyoni Matias de OliveiraAna Paula FerroGeorge Jackson da RochaMaria de Lourdes L FerrareseWanderley Dantas dos SantosOsvaldo Ferrarese-FilhoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e110000 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Flavia Carolina Moreira-Vilar Rita de Cássia Siqueira-Soares Aline Finger-Teixeira Dyoni Matias de Oliveira Ana Paula Ferro George Jackson da Rocha Maria de Lourdes L Ferrarese Wanderley Dantas dos Santos Osvaldo Ferrarese-Filho The acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than Klason and thioglycolic acid methods.
description	We compared the amount of lignin as determined by the three most traditional methods for lignin measurement in three tissues (sugarcane bagasse, soybean roots and soybean seed coat) contrasting for lignin amount and composition. Although all methods presented high reproducibility, major inconsistencies among them were found. The amount of lignin determined by thioglycolic acid method was severely lower than that provided by the other methods (up to 95%) in all tissues analyzed. Klason method was quite similar to acetyl bromide in tissues containing higher amounts of lignin, but presented lower recovery of lignin in the less lignified tissue. To investigate the causes of the inconsistencies observed, we determined the monomer composition of all plant materials, but found no correlation. We found that the low recovery of lignin presented by the thioglycolic acid method were due losses of lignin in the residues disposed throughout the procedures. The production of furfurals by acetyl bromide method does not explain the differences observed. The acetyl bromide method is the simplest and fastest among the methods evaluated presenting similar or best recovery of lignin in all the tissues assessed.
format	article
author	Flavia Carolina Moreira-Vilar Rita de Cássia Siqueira-Soares Aline Finger-Teixeira Dyoni Matias de Oliveira Ana Paula Ferro George Jackson da Rocha Maria de Lourdes L Ferrarese Wanderley Dantas dos Santos Osvaldo Ferrarese-Filho
author_facet	Flavia Carolina Moreira-Vilar Rita de Cássia Siqueira-Soares Aline Finger-Teixeira Dyoni Matias de Oliveira Ana Paula Ferro George Jackson da Rocha Maria de Lourdes L Ferrarese Wanderley Dantas dos Santos Osvaldo Ferrarese-Filho
author_sort	Flavia Carolina Moreira-Vilar
title	The acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than Klason and thioglycolic acid methods.
title_short	The acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than Klason and thioglycolic acid methods.
title_full	The acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than Klason and thioglycolic acid methods.
title_fullStr	The acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than Klason and thioglycolic acid methods.
title_full_unstemmed	The acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than Klason and thioglycolic acid methods.
title_sort	acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than klason and thioglycolic acid methods.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d8c889c93d8042f3aa125da74cc0a800
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The acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than Klason and thioglycolic acid methods.

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