Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of <i>Rubus matsumuranus</i> Phenolome

<i>Rubus matsumuranus</i> H. Lev. & Vaniot, a famous Siberian shrub of the Rosaceae family, is used in the folk medicine of nomads (Buryats, Yakuts, Soyots, and Mongols) as a remedy for the treatment of diseases of the respiratory and hepatobiliary systems. The lack of scientific inf...

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Autores principales:	Nina I. Kashchenko, Daniil N. Olennikov, Nadezhda K. Chirikova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Rubus matsumuranus</i> Rosaceae phenolic compounds ellagitannins high-performance liquid chromatography mass spectrometry Botany QK1-989
Acceso en línea:	https://doaj.org/article/d8c8e71d011d4d2d9de3fd6cab6d9ea8
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spelling	oai:doaj.org-article:d8c8e71d011d4d2d9de3fd6cab6d9ea82021-11-25T18:45:16ZMetabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of <i>Rubus matsumuranus</i> Phenolome10.3390/plants101123172223-7747https://doaj.org/article/d8c8e71d011d4d2d9de3fd6cab6d9ea82021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2317https://doaj.org/toc/2223-7747<i>Rubus matsumuranus</i> H. Lev. & Vaniot, a famous Siberian shrub of the Rosaceae family, is used in the folk medicine of nomads (Buryats, Yakuts, Soyots, and Mongols) as a remedy for the treatment of diseases of the respiratory and hepatobiliary systems. The lack of scientific information on <i>R. matsumuranus</i> leaves contributed to the investigation of the metabolomic profile and biological activity of this plant. In this study, metabolites of <i>R. matsumuranus</i> leaves in three stages (active growth, flowering, and fruiting) were characterised using high-performance liquid chromatography with photodiode array and electrospray ionisation triple quadrupole mass spectrometric detection (HPLC-PDA-ESI-tQ-MS). In total, 63 compounds were identified, including gallic acid derivatives, hydroxycinnamates, catechins, procyanidins, flavonols, and ellagitannins. Lambertianin C (57.11 mg/g of dry weight, DW), miquelianin (39.63 mg/g DW), and kaempferol-3-<i>O</i>-glucuronide (31.18 mg/g DW) were the major compounds in <i>R. matsumuranus</i> leaves. As a result of the HPLC-PDA-based assay to determine the antioxidant activity, it was revealed that lambertianin A, sanguiin H6, lambertianin C, and sanguiin H11 were effective scavengers of free radicals (2,2-diphenyl-1-picrylhydrazyl, DPPH<sup>•</sup>) and possessed Fe<sup>2+</sup>-chelating activity. After an investigation of the phenolic content in infusions and decoctions obtained by extraction with water at different temperatures, it was revealed that a hot infusion (80 °C) is a phenolic-rich preparation of <i>R. matsumuranus</i> leaves. Our research suggests that <i>R. matsumuranus</i> leaves are a rich source of phenolic compounds with high antioxidant properties and that this could be a prospective plant for new functional products.Nina I. KashchenkoDaniil N. OlennikovNadezhda K. ChirikovaMDPI AGarticle<i>Rubus matsumuranus</i>Rosaceaephenolic compoundsellagitanninshigh-performance liquid chromatographymass spectrometryBotanyQK1-989ENPlants, Vol 10, Iss 2317, p 2317 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Rubus matsumuranus</i> Rosaceae phenolic compounds ellagitannins high-performance liquid chromatography mass spectrometry Botany QK1-989
spellingShingle	<i>Rubus matsumuranus</i> Rosaceae phenolic compounds ellagitannins high-performance liquid chromatography mass spectrometry Botany QK1-989 Nina I. Kashchenko Daniil N. Olennikov Nadezhda K. Chirikova Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of <i>Rubus matsumuranus</i> Phenolome
description	<i>Rubus matsumuranus</i> H. Lev. & Vaniot, a famous Siberian shrub of the Rosaceae family, is used in the folk medicine of nomads (Buryats, Yakuts, Soyots, and Mongols) as a remedy for the treatment of diseases of the respiratory and hepatobiliary systems. The lack of scientific information on <i>R. matsumuranus</i> leaves contributed to the investigation of the metabolomic profile and biological activity of this plant. In this study, metabolites of <i>R. matsumuranus</i> leaves in three stages (active growth, flowering, and fruiting) were characterised using high-performance liquid chromatography with photodiode array and electrospray ionisation triple quadrupole mass spectrometric detection (HPLC-PDA-ESI-tQ-MS). In total, 63 compounds were identified, including gallic acid derivatives, hydroxycinnamates, catechins, procyanidins, flavonols, and ellagitannins. Lambertianin C (57.11 mg/g of dry weight, DW), miquelianin (39.63 mg/g DW), and kaempferol-3-<i>O</i>-glucuronide (31.18 mg/g DW) were the major compounds in <i>R. matsumuranus</i> leaves. As a result of the HPLC-PDA-based assay to determine the antioxidant activity, it was revealed that lambertianin A, sanguiin H6, lambertianin C, and sanguiin H11 were effective scavengers of free radicals (2,2-diphenyl-1-picrylhydrazyl, DPPH<sup>•</sup>) and possessed Fe<sup>2+</sup>-chelating activity. After an investigation of the phenolic content in infusions and decoctions obtained by extraction with water at different temperatures, it was revealed that a hot infusion (80 °C) is a phenolic-rich preparation of <i>R. matsumuranus</i> leaves. Our research suggests that <i>R. matsumuranus</i> leaves are a rich source of phenolic compounds with high antioxidant properties and that this could be a prospective plant for new functional products.
format	article
author	Nina I. Kashchenko Daniil N. Olennikov Nadezhda K. Chirikova
author_facet	Nina I. Kashchenko Daniil N. Olennikov Nadezhda K. Chirikova
author_sort	Nina I. Kashchenko
title	Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of <i>Rubus matsumuranus</i> Phenolome
title_short	Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of <i>Rubus matsumuranus</i> Phenolome
title_full	Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of <i>Rubus matsumuranus</i> Phenolome
title_fullStr	Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of <i>Rubus matsumuranus</i> Phenolome
title_full_unstemmed	Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of <i>Rubus matsumuranus</i> Phenolome
title_sort	metabolites of siberian raspberries: lc-ms profile, seasonal variation, antioxidant activity and, thermal stability of <i>rubus matsumuranus</i> phenolome
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d8c8e71d011d4d2d9de3fd6cab6d9ea8
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Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of <i>Rubus matsumuranus</i> Phenolome

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