Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering

Cell-based tissue engineering (TE) has been proposed to improve treatment outcomes in end-stage bladder disease, but TE approaches with 2D smooth muscle cell (SMC) culture have so far been unsuccessful. Here, we report the development of primary bladder-derived 3D SMC spheroids that outperform 2D SM...

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Autores principales:	Tim Gerwinn, Souzan Salemi, Lisa Krattiger, Daniel Eberli, Maya Horst
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/d8cd47add2394091826aef620a35f2f2
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spelling	oai:doaj.org-article:d8cd47add2394091826aef620a35f2f22021-11-22T01:10:33ZSpheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering2314-614110.1155/2021/9391575https://doaj.org/article/d8cd47add2394091826aef620a35f2f22021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/9391575https://doaj.org/toc/2314-6141Cell-based tissue engineering (TE) has been proposed to improve treatment outcomes in end-stage bladder disease, but TE approaches with 2D smooth muscle cell (SMC) culture have so far been unsuccessful. Here, we report the development of primary bladder-derived 3D SMC spheroids that outperform 2D SMC cultures in differentiation, maturation, and extracellular matrix (ECM) production. Bladder SMC spheroids were compared with 2D cultures using live-dead staining, qRT-PCR, immunofluorescence, and immunoblotting to investigate culture conditions, contractile phenotype, and ECM deposition. The SMC spheroids were viable for up to 14 days and differentiated rather than proliferating. Spheroids predominantly expressed the late myogenic differentiation marker MyH11, whereas 2D SMC expressed more of the general SMC differentiation marker α-SMA and less MyH11. Furthermore, the expression of bladder wall-specific ECM proteins in SMC spheroids was markedly higher. This first establishment and analysis of primary bladder SMC spheroids are particularly promising for TE because differentiated SMCs and ECM deposition are a prerequisite to building a functional bladder wall substitute. We were able to confirm that SMC spheroids are promising building blocks for studying detrusor regeneration in detail and may provide improved function and regenerative potential, contributing to taking bladder TE a significant step forward.Tim GerwinnSouzan SalemiLisa KrattigerDaniel EberliMaya HorstHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Tim Gerwinn Souzan Salemi Lisa Krattiger Daniel Eberli Maya Horst Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering
description	Cell-based tissue engineering (TE) has been proposed to improve treatment outcomes in end-stage bladder disease, but TE approaches with 2D smooth muscle cell (SMC) culture have so far been unsuccessful. Here, we report the development of primary bladder-derived 3D SMC spheroids that outperform 2D SMC cultures in differentiation, maturation, and extracellular matrix (ECM) production. Bladder SMC spheroids were compared with 2D cultures using live-dead staining, qRT-PCR, immunofluorescence, and immunoblotting to investigate culture conditions, contractile phenotype, and ECM deposition. The SMC spheroids were viable for up to 14 days and differentiated rather than proliferating. Spheroids predominantly expressed the late myogenic differentiation marker MyH11, whereas 2D SMC expressed more of the general SMC differentiation marker α-SMA and less MyH11. Furthermore, the expression of bladder wall-specific ECM proteins in SMC spheroids was markedly higher. This first establishment and analysis of primary bladder SMC spheroids are particularly promising for TE because differentiated SMCs and ECM deposition are a prerequisite to building a functional bladder wall substitute. We were able to confirm that SMC spheroids are promising building blocks for studying detrusor regeneration in detail and may provide improved function and regenerative potential, contributing to taking bladder TE a significant step forward.
format	article
author	Tim Gerwinn Souzan Salemi Lisa Krattiger Daniel Eberli Maya Horst
author_facet	Tim Gerwinn Souzan Salemi Lisa Krattiger Daniel Eberli Maya Horst
author_sort	Tim Gerwinn
title	Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering
title_short	Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering
title_full	Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering
title_fullStr	Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering
title_full_unstemmed	Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering
title_sort	spheroids of bladder smooth muscle cells for bladder tissue engineering
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/d8cd47add2394091826aef620a35f2f2
work_keys_str_mv	AT timgerwinn spheroidsofbladdersmoothmusclecellsforbladdertissueengineering AT souzansalemi spheroidsofbladdersmoothmusclecellsforbladdertissueengineering AT lisakrattiger spheroidsofbladdersmoothmusclecellsforbladdertissueengineering AT danieleberli spheroidsofbladdersmoothmusclecellsforbladdertissueengineering AT mayahorst spheroidsofbladdersmoothmusclecellsforbladdertissueengineering
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Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering

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