Effect of Dextromethorphan on the neuromuscular transmission of the chick biventer cervicis muscle preparation

Background and Objective: Dextromethorphan is a well-known over-the-counter antitussive drug with over 45 years of clinical usage experience. Its toxicity is low, but extremely high doses may produce muscle or CNS depression, drowsiness and gastrointestinal upset. In the present study, the effect of...

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Autores principales:	D Farzin, M Zavareh
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2003
Materias:	chick biventer cervicis muscle dextromethorphan caffeine Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d8d125a658614a9a8fc935bc9922668d
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spelling	oai:doaj.org-article:d8d125a658614a9a8fc935bc9922668d2021-11-10T09:18:21ZEffect of Dextromethorphan on the neuromuscular transmission of the chick biventer cervicis muscle preparation1561-41072251-7170https://doaj.org/article/d8d125a658614a9a8fc935bc9922668d2003-10-01T00:00:00Zhttp://jbums.org/article-1-2744-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170Background and Objective: Dextromethorphan is a well-known over-the-counter antitussive drug with over 45 years of clinical usage experience. Its toxicity is low, but extremely high doses may produce muscle or CNS depression, drowsiness and gastrointestinal upset. In the present study, the effect of dextromethorphan on the neuromuscular transmission of isolated chick biventer cervicis muscle preparation was examined. Methods: This isolated chick (About 3 weeks old) biventer cervicis muscle was put in the organ bath. The organ bath had a vessel with volume of about 70ml, it contained tyrode solution aerated with oxygen and was kept at 37°C. The nerve supplying the twitch-fibers was located in the tendon against which the electrodes were placed. It was stimulated usually at a frequency of 0.1 HZ, duration of 0.5 msec. and voltage of 5-10 volt and twitch response was recorded by a polygraph apparatus after transferring through a transducer. Findings: Dextromethorphan in the range of 2.5 to 5.4 µM has an inhibitory effect on the twitch response to indirect electrical stimuli and contracture of exogenous acetylcholine. The inhibitory effect of dextromethorphan was not antagonized by physostigmine or 4-aminopyridin. Potentiation to twitch response due to 10 fold greater duration of the stimuli was decreased by dextromethorphan. Both tetanosis and facilitating after tetanosis were inhibited in the presence of dextromethorphan. Twitch response to the direct electrical stimuli was inhibited by dextromethorphan. Caffeine significantly antagonized the inhibitory effect of dextromethorphan on the twitch response to indirect electrical stimuli. Dose-response curves of acetylcholine and carbachol in the presence of dextromethorphan shifted to right with a decrease in the efficacy. Conclusion: These results suggested that the inhibitory effect of dextromethorphan on the neuromuscular transmission of the chick biventer cervicis muscle is mediated through blockade of sarcoplasmic reticulum calcium channels.D FarzinM ZavarehBabol University of Medical Sciencesarticlechick biventer cervicis muscledextromethorphancaffeineMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 5, Iss 4, Pp 11-17 (2003)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	chick biventer cervicis muscle dextromethorphan caffeine Medicine R Medicine (General) R5-920
spellingShingle	chick biventer cervicis muscle dextromethorphan caffeine Medicine R Medicine (General) R5-920 D Farzin M Zavareh Effect of Dextromethorphan on the neuromuscular transmission of the chick biventer cervicis muscle preparation
description	Background and Objective: Dextromethorphan is a well-known over-the-counter antitussive drug with over 45 years of clinical usage experience. Its toxicity is low, but extremely high doses may produce muscle or CNS depression, drowsiness and gastrointestinal upset. In the present study, the effect of dextromethorphan on the neuromuscular transmission of isolated chick biventer cervicis muscle preparation was examined. Methods: This isolated chick (About 3 weeks old) biventer cervicis muscle was put in the organ bath. The organ bath had a vessel with volume of about 70ml, it contained tyrode solution aerated with oxygen and was kept at 37°C. The nerve supplying the twitch-fibers was located in the tendon against which the electrodes were placed. It was stimulated usually at a frequency of 0.1 HZ, duration of 0.5 msec. and voltage of 5-10 volt and twitch response was recorded by a polygraph apparatus after transferring through a transducer. Findings: Dextromethorphan in the range of 2.5 to 5.4 µM has an inhibitory effect on the twitch response to indirect electrical stimuli and contracture of exogenous acetylcholine. The inhibitory effect of dextromethorphan was not antagonized by physostigmine or 4-aminopyridin. Potentiation to twitch response due to 10 fold greater duration of the stimuli was decreased by dextromethorphan. Both tetanosis and facilitating after tetanosis were inhibited in the presence of dextromethorphan. Twitch response to the direct electrical stimuli was inhibited by dextromethorphan. Caffeine significantly antagonized the inhibitory effect of dextromethorphan on the twitch response to indirect electrical stimuli. Dose-response curves of acetylcholine and carbachol in the presence of dextromethorphan shifted to right with a decrease in the efficacy. Conclusion: These results suggested that the inhibitory effect of dextromethorphan on the neuromuscular transmission of the chick biventer cervicis muscle is mediated through blockade of sarcoplasmic reticulum calcium channels.
format	article
author	D Farzin M Zavareh
author_facet	D Farzin M Zavareh
author_sort	D Farzin
title	Effect of Dextromethorphan on the neuromuscular transmission of the chick biventer cervicis muscle preparation
title_short	Effect of Dextromethorphan on the neuromuscular transmission of the chick biventer cervicis muscle preparation
title_full	Effect of Dextromethorphan on the neuromuscular transmission of the chick biventer cervicis muscle preparation
title_fullStr	Effect of Dextromethorphan on the neuromuscular transmission of the chick biventer cervicis muscle preparation
title_full_unstemmed	Effect of Dextromethorphan on the neuromuscular transmission of the chick biventer cervicis muscle preparation
title_sort	effect of dextromethorphan on the neuromuscular transmission of the chick biventer cervicis muscle preparation
publisher	Babol University of Medical Sciences
publishDate	2003
url	https://doaj.org/article/d8d125a658614a9a8fc935bc9922668d
work_keys_str_mv	AT dfarzin effectofdextromethorphanontheneuromusculartransmissionofthechickbiventercervicismusclepreparation AT mzavareh effectofdextromethorphanontheneuromusculartransmissionofthechickbiventercervicismusclepreparation
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Effect of Dextromethorphan on the neuromuscular transmission of the chick biventer cervicis muscle preparation

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