Molecular and phenotypic characterization of avian pathogenic Escherichia coli isolated from commercial broilers and native chickens

ABSTRACT: Many studies have examined avian pathogenic Escherichia coli (APEC) from commercial broilers but few have examined isolates from native chickens. This study compared APEC isolates from commercial broilers and native chickens in regard to the phylogenetic group and the phenotypic and genoty...

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Autores principales:	Thotsapol Thomrongsuwannakij, Ruethai Narinthorn, Tanakamol Mahawan, Patrick J. Blackall
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	avian pathogenic Escherichia coli antimicrobial resistance commercial broiler native chicken resistance gene Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/d8db299db4b14d8085ce0b3219ac9baf
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spelling	oai:doaj.org-article:d8db299db4b14d8085ce0b3219ac9baf2021-11-24T04:23:00ZMolecular and phenotypic characterization of avian pathogenic Escherichia coli isolated from commercial broilers and native chickens0032-579110.1016/j.psj.2021.101527https://doaj.org/article/d8db299db4b14d8085ce0b3219ac9baf2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005496https://doaj.org/toc/0032-5791ABSTRACT: Many studies have examined avian pathogenic Escherichia coli (APEC) from commercial broilers but few have examined isolates from native chickens. This study compared APEC isolates from commercial broilers and native chickens in regard to the phylogenetic group and the phenotypic and genotypic antimicrobial resistance profiles. From 100 suspect colibacillosis cases in both commercial broilers and native chickens, a total of 90 broiler isolates and 42 native chicken isolates were identified as E. coli by biochemical tests. Phylogenetic grouping revealed that 90 broiler APEC isolates belonged to A group (5.56%), B1 group (22.22%), B2 group (31.11%), and D group (41.11%). The 42 native chicken APEC isolates belonged to A group (35.71%), B1 group (26.19%), B2 group (30.95%), and D group (7.14%). The difference in the allocation to groups A and D of the 2 isolate types was significant (P < 0.05). The APEC broiler isolates had a significantly higher multidrug-resistant (MDR) rate (80%) than the native chicken isolates (14.29%) (P < 0.05). The APEC broiler isolates demonstrated significantly higher resistance rates than the native chicken isolates for amoxicillin (98.89%; 78.57% respectively), chloramphenicol (42.2%; 9.5%), enrofloxacin (68.9%; 7.1%), gentamicin (11.1%; 0%), nalidixic acid (72.2%; 7.1%), sulfamethoxazole + trimethoprim (45.6%; 2.4%), and tetracycline (88.9%; 76.2%) (P < 0.05). The APEC broiler isolates had a significantly higher presence compared with the native chicken isolates of the following resistance genes:- by blaTEM (43.3%; 21.4%, respectively), cml-A (34.4%; 2.4%), tetA (76.7%; 40.5%), tetB (26.7%; 0%), sul2 (23.3%; 14.3%), and dhfrI (13.3%; 0%) (P < 0.05). The qnrB and qnrS genes were detected (12.16%; 72.97% respectively), in the APEC broiler isolates resistant to nalidixic acid and/or enrofloxacin while only qnrS genes was detected in all 3 APEC native chicken isolates. Regarding the point mutations of gyrA and parC, all isolates were positive to gyrA83S, gyrA87D, gyrA87L, gyrA87NY, parC80S and parC80I except that gyrA83S was not present in 20 APEC broiler isolates. Antimicrobial stewardship programs should be targeted at the backyard poultry sector as well as the commercial poultry sector.Thotsapol ThomrongsuwannakijRuethai NarinthornTanakamol MahawanPatrick J. BlackallElsevierarticleavian pathogenic Escherichia coliantimicrobial resistancecommercial broilernative chickenresistance geneAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101527- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	avian pathogenic Escherichia coli antimicrobial resistance commercial broiler native chicken resistance gene Animal culture SF1-1100
spellingShingle	avian pathogenic Escherichia coli antimicrobial resistance commercial broiler native chicken resistance gene Animal culture SF1-1100 Thotsapol Thomrongsuwannakij Ruethai Narinthorn Tanakamol Mahawan Patrick J. Blackall Molecular and phenotypic characterization of avian pathogenic Escherichia coli isolated from commercial broilers and native chickens
description	ABSTRACT: Many studies have examined avian pathogenic Escherichia coli (APEC) from commercial broilers but few have examined isolates from native chickens. This study compared APEC isolates from commercial broilers and native chickens in regard to the phylogenetic group and the phenotypic and genotypic antimicrobial resistance profiles. From 100 suspect colibacillosis cases in both commercial broilers and native chickens, a total of 90 broiler isolates and 42 native chicken isolates were identified as E. coli by biochemical tests. Phylogenetic grouping revealed that 90 broiler APEC isolates belonged to A group (5.56%), B1 group (22.22%), B2 group (31.11%), and D group (41.11%). The 42 native chicken APEC isolates belonged to A group (35.71%), B1 group (26.19%), B2 group (30.95%), and D group (7.14%). The difference in the allocation to groups A and D of the 2 isolate types was significant (P < 0.05). The APEC broiler isolates had a significantly higher multidrug-resistant (MDR) rate (80%) than the native chicken isolates (14.29%) (P < 0.05). The APEC broiler isolates demonstrated significantly higher resistance rates than the native chicken isolates for amoxicillin (98.89%; 78.57% respectively), chloramphenicol (42.2%; 9.5%), enrofloxacin (68.9%; 7.1%), gentamicin (11.1%; 0%), nalidixic acid (72.2%; 7.1%), sulfamethoxazole + trimethoprim (45.6%; 2.4%), and tetracycline (88.9%; 76.2%) (P < 0.05). The APEC broiler isolates had a significantly higher presence compared with the native chicken isolates of the following resistance genes:- by blaTEM (43.3%; 21.4%, respectively), cml-A (34.4%; 2.4%), tetA (76.7%; 40.5%), tetB (26.7%; 0%), sul2 (23.3%; 14.3%), and dhfrI (13.3%; 0%) (P < 0.05). The qnrB and qnrS genes were detected (12.16%; 72.97% respectively), in the APEC broiler isolates resistant to nalidixic acid and/or enrofloxacin while only qnrS genes was detected in all 3 APEC native chicken isolates. Regarding the point mutations of gyrA and parC, all isolates were positive to gyrA83S, gyrA87D, gyrA87L, gyrA87NY, parC80S and parC80I except that gyrA83S was not present in 20 APEC broiler isolates. Antimicrobial stewardship programs should be targeted at the backyard poultry sector as well as the commercial poultry sector.
format	article
author	Thotsapol Thomrongsuwannakij Ruethai Narinthorn Tanakamol Mahawan Patrick J. Blackall
author_facet	Thotsapol Thomrongsuwannakij Ruethai Narinthorn Tanakamol Mahawan Patrick J. Blackall
author_sort	Thotsapol Thomrongsuwannakij
title	Molecular and phenotypic characterization of avian pathogenic Escherichia coli isolated from commercial broilers and native chickens
title_short	Molecular and phenotypic characterization of avian pathogenic Escherichia coli isolated from commercial broilers and native chickens
title_full	Molecular and phenotypic characterization of avian pathogenic Escherichia coli isolated from commercial broilers and native chickens
title_fullStr	Molecular and phenotypic characterization of avian pathogenic Escherichia coli isolated from commercial broilers and native chickens
title_full_unstemmed	Molecular and phenotypic characterization of avian pathogenic Escherichia coli isolated from commercial broilers and native chickens
title_sort	molecular and phenotypic characterization of avian pathogenic escherichia coli isolated from commercial broilers and native chickens
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/d8db299db4b14d8085ce0b3219ac9baf
work_keys_str_mv	AT thotsapolthomrongsuwannakij molecularandphenotypiccharacterizationofavianpathogenicescherichiacoliisolatedfromcommercialbroilersandnativechickens AT ruethainarinthorn molecularandphenotypiccharacterizationofavianpathogenicescherichiacoliisolatedfromcommercialbroilersandnativechickens AT tanakamolmahawan molecularandphenotypiccharacterizationofavianpathogenicescherichiacoliisolatedfromcommercialbroilersandnativechickens AT patrickjblackall molecularandphenotypiccharacterizationofavianpathogenicescherichiacoliisolatedfromcommercialbroilersandnativechickens
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Molecular and phenotypic characterization of avian pathogenic Escherichia coli isolated from commercial broilers and native chickens

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