Sustainable biosynthesis of chemicals from methane and glycerol via reconstruction of multi‐carbon utilizing pathway in obligate methanotrophic bacteria

Summary Obligate methanotrophic bacteria can utilize methane, an inexpensive carbon feedstock, as a sole energy and carbon substrate, thus are considered as the only nature‐provided biocatalyst for sustainable biomanufacturing of fuels and chemicals from methane. To address the limitation of native...

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Autores principales:	Hoa Thi Quynh Le, Anh Duc Nguyen, Ye Rim Park, Eun Yeol Lee
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/d8e59d95fb944efcb648e78e83e076c4
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spelling	oai:doaj.org-article:d8e59d95fb944efcb648e78e83e076c42021-11-18T15:39:53ZSustainable biosynthesis of chemicals from methane and glycerol via reconstruction of multi‐carbon utilizing pathway in obligate methanotrophic bacteria1751-791510.1111/1751-7915.13809https://doaj.org/article/d8e59d95fb944efcb648e78e83e076c42021-11-01T00:00:00Zhttps://doi.org/10.1111/1751-7915.13809https://doaj.org/toc/1751-7915Summary Obligate methanotrophic bacteria can utilize methane, an inexpensive carbon feedstock, as a sole energy and carbon substrate, thus are considered as the only nature‐provided biocatalyst for sustainable biomanufacturing of fuels and chemicals from methane. To address the limitation of native C1 metabolism of obligate type I methanotrophs, we proposed a novel platform strain that can utilize methane and multi‐carbon substrates, such as glycerol, simultaneously to boost growth rates and chemical production in Methylotuvimicrobium alcaliphilum 20Z. To demonstrate the uses of this concept, we reconstructed a 2,3‐butanediol biosynthetic pathway and achieved a fourfold higher titer of 2,3‐butanediol production by co‐utilizing methane and glycerol compared with that of methanotrophic growth. In addition, we reported the creation of a methanotrophic biocatalyst for one‐step bioconversion of methane to methanol in which glycerol was used for cell growth, and methane was mainly used for methanol production. After the deletion of genes encoding methanol dehydrogenase (MDH), 11.6 mM methanol was obtained after 72 h using living cells in the absence of any chemical inhibitors of MDH and exogenous NADH source. A further improvement of this bioconversion was attained by using resting cells with a significantly increased titre of 76 mM methanol after 3.5 h with the supply of 40 mM formate. The work presented here provides a novel framework for a variety of approaches in methane‐based biomanufacturing.Hoa Thi Quynh LeAnh Duc NguyenYe Rim ParkEun Yeol LeeWileyarticleBiotechnologyTP248.13-248.65ENMicrobial Biotechnology, Vol 14, Iss 6, Pp 2552-2565 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65
spellingShingle	Biotechnology TP248.13-248.65 Hoa Thi Quynh Le Anh Duc Nguyen Ye Rim Park Eun Yeol Lee Sustainable biosynthesis of chemicals from methane and glycerol via reconstruction of multi‐carbon utilizing pathway in obligate methanotrophic bacteria
description	Summary Obligate methanotrophic bacteria can utilize methane, an inexpensive carbon feedstock, as a sole energy and carbon substrate, thus are considered as the only nature‐provided biocatalyst for sustainable biomanufacturing of fuels and chemicals from methane. To address the limitation of native C1 metabolism of obligate type I methanotrophs, we proposed a novel platform strain that can utilize methane and multi‐carbon substrates, such as glycerol, simultaneously to boost growth rates and chemical production in Methylotuvimicrobium alcaliphilum 20Z. To demonstrate the uses of this concept, we reconstructed a 2,3‐butanediol biosynthetic pathway and achieved a fourfold higher titer of 2,3‐butanediol production by co‐utilizing methane and glycerol compared with that of methanotrophic growth. In addition, we reported the creation of a methanotrophic biocatalyst for one‐step bioconversion of methane to methanol in which glycerol was used for cell growth, and methane was mainly used for methanol production. After the deletion of genes encoding methanol dehydrogenase (MDH), 11.6 mM methanol was obtained after 72 h using living cells in the absence of any chemical inhibitors of MDH and exogenous NADH source. A further improvement of this bioconversion was attained by using resting cells with a significantly increased titre of 76 mM methanol after 3.5 h with the supply of 40 mM formate. The work presented here provides a novel framework for a variety of approaches in methane‐based biomanufacturing.
format	article
author	Hoa Thi Quynh Le Anh Duc Nguyen Ye Rim Park Eun Yeol Lee
author_facet	Hoa Thi Quynh Le Anh Duc Nguyen Ye Rim Park Eun Yeol Lee
author_sort	Hoa Thi Quynh Le
title	Sustainable biosynthesis of chemicals from methane and glycerol via reconstruction of multi‐carbon utilizing pathway in obligate methanotrophic bacteria
title_short	Sustainable biosynthesis of chemicals from methane and glycerol via reconstruction of multi‐carbon utilizing pathway in obligate methanotrophic bacteria
title_full	Sustainable biosynthesis of chemicals from methane and glycerol via reconstruction of multi‐carbon utilizing pathway in obligate methanotrophic bacteria
title_fullStr	Sustainable biosynthesis of chemicals from methane and glycerol via reconstruction of multi‐carbon utilizing pathway in obligate methanotrophic bacteria
title_full_unstemmed	Sustainable biosynthesis of chemicals from methane and glycerol via reconstruction of multi‐carbon utilizing pathway in obligate methanotrophic bacteria
title_sort	sustainable biosynthesis of chemicals from methane and glycerol via reconstruction of multi‐carbon utilizing pathway in obligate methanotrophic bacteria
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/d8e59d95fb944efcb648e78e83e076c4
work_keys_str_mv	AT hoathiquynhle sustainablebiosynthesisofchemicalsfrommethaneandglycerolviareconstructionofmulticarbonutilizingpathwayinobligatemethanotrophicbacteria AT anhducnguyen sustainablebiosynthesisofchemicalsfrommethaneandglycerolviareconstructionofmulticarbonutilizingpathwayinobligatemethanotrophicbacteria AT yerimpark sustainablebiosynthesisofchemicalsfrommethaneandglycerolviareconstructionofmulticarbonutilizingpathwayinobligatemethanotrophicbacteria AT eunyeollee sustainablebiosynthesisofchemicalsfrommethaneandglycerolviareconstructionofmulticarbonutilizingpathwayinobligatemethanotrophicbacteria
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Sustainable biosynthesis of chemicals from methane and glycerol via reconstruction of multi‐carbon utilizing pathway in obligate methanotrophic bacteria

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