The 3'tsRNAs are aminoacylated: Implications for their biogenesis.

Emerging evidence indicates that tRNA-derived small RNAs (tsRNAs) are involved in fine-tuning gene expression and become dysregulated in various cancers. We recently showed that the 22nt LeuCAG3´tsRNA from the 3´ end of tRNALeu is required for efficient translation of a ribosomal protein mRNA and ri...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ziwei Liu, Hak Kyun Kim, Jianpeng Xu, Yuqing Jing, Mark A Kay
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/d8e63fe89a604e29b711a5b65e05ab46
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d8e63fe89a604e29b711a5b65e05ab46
record_format	dspace
spelling	oai:doaj.org-article:d8e63fe89a604e29b711a5b65e05ab462021-12-02T20:02:54ZThe 3'tsRNAs are aminoacylated: Implications for their biogenesis.1553-73901553-740410.1371/journal.pgen.1009675https://doaj.org/article/d8e63fe89a604e29b711a5b65e05ab462021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009675https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Emerging evidence indicates that tRNA-derived small RNAs (tsRNAs) are involved in fine-tuning gene expression and become dysregulated in various cancers. We recently showed that the 22nt LeuCAG3´tsRNA from the 3´ end of tRNALeu is required for efficient translation of a ribosomal protein mRNA and ribosome biogenesis. Inactivation of this 3´tsRNA induced apoptosis in rapidly dividing cells and suppressed the growth of a patient-derived orthotopic hepatocellular carcinoma in mice. The mechanism involved in the generation of the 3´tsRNAs remains elusive and it is unclear if the 3´-ends of 3´tsRNAs are aminoacylated. Here we report an enzymatic method utilizing exonuclease T to determine the 3´charging status of tRNAs and tsRNAs. Our results showed that the LeuCAG3´tsRNA, and two other 3´tsRNAs are fully aminoacylated. When the leucyl-tRNA synthetase (LARS1) was inhibited, there was no change in the total tRNALeu concentration but a reduction in both the charged tRNALeu and LeuCAG3´tsRNA, suggesting the 3´tsRNAs are fully charged and originated solely from the charged mature tRNA. Altering LARS1 expression or the expression of various tRNALeu mutants were also shown to affect the generation of the LeuCAG3´tsRNA further suggesting they are created in a highly regulated process. The fact that the 3´tsRNAs are aminoacylated and their production is regulated provides additional insights into their importance in post-transcriptional gene regulation that includes coordinating the production of the protein synthetic machinery.Ziwei LiuHak Kyun KimJianpeng XuYuqing JingMark A KayPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 7, p e1009675 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Ziwei Liu Hak Kyun Kim Jianpeng Xu Yuqing Jing Mark A Kay The 3'tsRNAs are aminoacylated: Implications for their biogenesis.
description	Emerging evidence indicates that tRNA-derived small RNAs (tsRNAs) are involved in fine-tuning gene expression and become dysregulated in various cancers. We recently showed that the 22nt LeuCAG3´tsRNA from the 3´ end of tRNALeu is required for efficient translation of a ribosomal protein mRNA and ribosome biogenesis. Inactivation of this 3´tsRNA induced apoptosis in rapidly dividing cells and suppressed the growth of a patient-derived orthotopic hepatocellular carcinoma in mice. The mechanism involved in the generation of the 3´tsRNAs remains elusive and it is unclear if the 3´-ends of 3´tsRNAs are aminoacylated. Here we report an enzymatic method utilizing exonuclease T to determine the 3´charging status of tRNAs and tsRNAs. Our results showed that the LeuCAG3´tsRNA, and two other 3´tsRNAs are fully aminoacylated. When the leucyl-tRNA synthetase (LARS1) was inhibited, there was no change in the total tRNALeu concentration but a reduction in both the charged tRNALeu and LeuCAG3´tsRNA, suggesting the 3´tsRNAs are fully charged and originated solely from the charged mature tRNA. Altering LARS1 expression or the expression of various tRNALeu mutants were also shown to affect the generation of the LeuCAG3´tsRNA further suggesting they are created in a highly regulated process. The fact that the 3´tsRNAs are aminoacylated and their production is regulated provides additional insights into their importance in post-transcriptional gene regulation that includes coordinating the production of the protein synthetic machinery.
format	article
author	Ziwei Liu Hak Kyun Kim Jianpeng Xu Yuqing Jing Mark A Kay
author_facet	Ziwei Liu Hak Kyun Kim Jianpeng Xu Yuqing Jing Mark A Kay
author_sort	Ziwei Liu
title	The 3'tsRNAs are aminoacylated: Implications for their biogenesis.
title_short	The 3'tsRNAs are aminoacylated: Implications for their biogenesis.
title_full	The 3'tsRNAs are aminoacylated: Implications for their biogenesis.
title_fullStr	The 3'tsRNAs are aminoacylated: Implications for their biogenesis.
title_full_unstemmed	The 3'tsRNAs are aminoacylated: Implications for their biogenesis.
title_sort	3'tsrnas are aminoacylated: implications for their biogenesis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/d8e63fe89a604e29b711a5b65e05ab46
work_keys_str_mv	AT ziweiliu the3tsrnasareaminoacylatedimplicationsfortheirbiogenesis AT hakkyunkim the3tsrnasareaminoacylatedimplicationsfortheirbiogenesis AT jianpengxu the3tsrnasareaminoacylatedimplicationsfortheirbiogenesis AT yuqingjing the3tsrnasareaminoacylatedimplicationsfortheirbiogenesis AT markakay the3tsrnasareaminoacylatedimplicationsfortheirbiogenesis AT ziweiliu 3tsrnasareaminoacylatedimplicationsfortheirbiogenesis AT hakkyunkim 3tsrnasareaminoacylatedimplicationsfortheirbiogenesis AT jianpengxu 3tsrnasareaminoacylatedimplicationsfortheirbiogenesis AT yuqingjing 3tsrnasareaminoacylatedimplicationsfortheirbiogenesis AT markakay 3tsrnasareaminoacylatedimplicationsfortheirbiogenesis
_version_	1718375690503454720

The 3'tsRNAs are aminoacylated: Implications for their biogenesis.

Ejemplares similares