Multicenter evaluation of Verigene Enteric Pathogens Nucleic Acid Test for detection of gastrointestinal pathogens

Abstract We investigated the efficiency of the Verigene Enteric Pathogens Nucleic Acid Test (Verigene EP test), which is an automated microarray-based assay system that enables rapid and simultaneous genetic detection of gastrointestinal pathogens and toxins, including those in the Campylobacter Gro...

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Autores principales:	Kosuke Kosai, Hiromichi Suzuki, Kiyoko Tamai, Yuya Okada, Norihiko Akamatsu, Atsuo Ueda, Shigeyuki Notake, Yuji Yaguchi, Katsunori Yanagihara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d8e9103275ee4137b5a2586049a65348
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spelling	oai:doaj.org-article:d8e9103275ee4137b5a2586049a653482021-12-02T14:06:50ZMulticenter evaluation of Verigene Enteric Pathogens Nucleic Acid Test for detection of gastrointestinal pathogens10.1038/s41598-021-82490-z2045-2322https://doaj.org/article/d8e9103275ee4137b5a2586049a653482021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82490-zhttps://doaj.org/toc/2045-2322Abstract We investigated the efficiency of the Verigene Enteric Pathogens Nucleic Acid Test (Verigene EP test), which is an automated microarray-based assay system that enables rapid and simultaneous genetic detection of gastrointestinal pathogens and toxins, including those in the Campylobacter Group, Salmonella species, Shigella species, the Vibrio Group, Yersinia enterocolitica, Shiga toxin 1 and 2, norovirus GI/GII, and rotavirus A. Three clinical laboratories evaluated the Verigene EP test, using 268 stool samples for bacterial and toxin genes and 167 samples for viral genes. Culture-based reference methods were used for the detection of bacteria and toxins, while a different molecular assay was used for viral detection. The overall concordance rate between the Verigene EP test and the reference methods for the 1940 assays was 99.0%. The overall sensitivity and specificity of the Verigene EP test were 97.0% and 99.3%, respectively. Of the 19 samples with discordant results, 13 samples were false positives and six were false negatives. The Verigene EP test simultaneously detected two targets in 11 samples; overall, the test demonstrated high efficiency in detecting crucial diarrheagenic pathogens, indicating its suitability for clinical practice.Kosuke KosaiHiromichi SuzukiKiyoko TamaiYuya OkadaNorihiko AkamatsuAtsuo UedaShigeyuki NotakeYuji YaguchiKatsunori YanagiharaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kosuke Kosai Hiromichi Suzuki Kiyoko Tamai Yuya Okada Norihiko Akamatsu Atsuo Ueda Shigeyuki Notake Yuji Yaguchi Katsunori Yanagihara Multicenter evaluation of Verigene Enteric Pathogens Nucleic Acid Test for detection of gastrointestinal pathogens
description	Abstract We investigated the efficiency of the Verigene Enteric Pathogens Nucleic Acid Test (Verigene EP test), which is an automated microarray-based assay system that enables rapid and simultaneous genetic detection of gastrointestinal pathogens and toxins, including those in the Campylobacter Group, Salmonella species, Shigella species, the Vibrio Group, Yersinia enterocolitica, Shiga toxin 1 and 2, norovirus GI/GII, and rotavirus A. Three clinical laboratories evaluated the Verigene EP test, using 268 stool samples for bacterial and toxin genes and 167 samples for viral genes. Culture-based reference methods were used for the detection of bacteria and toxins, while a different molecular assay was used for viral detection. The overall concordance rate between the Verigene EP test and the reference methods for the 1940 assays was 99.0%. The overall sensitivity and specificity of the Verigene EP test were 97.0% and 99.3%, respectively. Of the 19 samples with discordant results, 13 samples were false positives and six were false negatives. The Verigene EP test simultaneously detected two targets in 11 samples; overall, the test demonstrated high efficiency in detecting crucial diarrheagenic pathogens, indicating its suitability for clinical practice.
format	article
author	Kosuke Kosai Hiromichi Suzuki Kiyoko Tamai Yuya Okada Norihiko Akamatsu Atsuo Ueda Shigeyuki Notake Yuji Yaguchi Katsunori Yanagihara
author_facet	Kosuke Kosai Hiromichi Suzuki Kiyoko Tamai Yuya Okada Norihiko Akamatsu Atsuo Ueda Shigeyuki Notake Yuji Yaguchi Katsunori Yanagihara
author_sort	Kosuke Kosai
title	Multicenter evaluation of Verigene Enteric Pathogens Nucleic Acid Test for detection of gastrointestinal pathogens
title_short	Multicenter evaluation of Verigene Enteric Pathogens Nucleic Acid Test for detection of gastrointestinal pathogens
title_full	Multicenter evaluation of Verigene Enteric Pathogens Nucleic Acid Test for detection of gastrointestinal pathogens
title_fullStr	Multicenter evaluation of Verigene Enteric Pathogens Nucleic Acid Test for detection of gastrointestinal pathogens
title_full_unstemmed	Multicenter evaluation of Verigene Enteric Pathogens Nucleic Acid Test for detection of gastrointestinal pathogens
title_sort	multicenter evaluation of verigene enteric pathogens nucleic acid test for detection of gastrointestinal pathogens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d8e9103275ee4137b5a2586049a65348
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Multicenter evaluation of Verigene Enteric Pathogens Nucleic Acid Test for detection of gastrointestinal pathogens

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