Chitosan based microcarriers for cellular growth and biologics production

Microcarrier technology has emerged as one of the promising platforms for large scale production of anchorage dependent cells. Cells grown on these carriers have been utilized for applications ranging from tissue engineering to vaccine production. Porous chitosan microcarriers (CMC), having diameter...

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Autores principales:	Tejal Pant, Vidhi Murarka, Ratnesh Jain, Prajakta Dandekar
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Chitosan Microcarriers Collagen Gelatin IgG production Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/d8ea62bf53964f96845bdd8bf3be902f
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spelling	oai:doaj.org-article:d8ea62bf53964f96845bdd8bf3be902f2021-11-04T04:43:18ZChitosan based microcarriers for cellular growth and biologics production2666-893910.1016/j.carpta.2021.100154https://doaj.org/article/d8ea62bf53964f96845bdd8bf3be902f2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666893921001225https://doaj.org/toc/2666-8939Microcarrier technology has emerged as one of the promising platforms for large scale production of anchorage dependent cells. Cells grown on these carriers have been utilized for applications ranging from tissue engineering to vaccine production. Porous chitosan microcarriers (CMC), having diameter in the range of 250 – 300 µm and having suitability for cell adhesion and proliferation, were prepared and evaluated in this study. CMC were cross-linked by ionic gelation method, using sodium tripolyphosphate, and freeze dried to obtain porous structures. Further, CMC were coated with an extracellular matrix (ECM) component, collagen, and its derivative, gelatin. Subsequently, the ability of coated and uncoated CMC to support the growth of CHO cells secreting human immunoglobulin G (IgG) was evaluated qualitatively and quantitatively by fluorescently labeling live cells and determining metabolically active cells respectively. IgG secretion from these cells was determined as a measure of cell functionality. After culturing cell laden CMC for 11 days, uncoated carriers were found to be more favorable for both, cell growth and productivity. The IgG productivity was 97.85, 74.26 and 84.12 ng/ mL for uncoated, collagen and gelatin coated CMC. CMC, based on its intrinsic properties and without the necessity of surface modifications, acted as a promising support matrix for culturing functionally active adherent cells, in suspension culture.Tejal PantVidhi MurarkaRatnesh JainPrajakta DandekarElsevierarticleChitosanMicrocarriersCollagenGelatinIgG productionBiochemistryQD415-436ENCarbohydrate Polymer Technologies and Applications, Vol 2, Iss , Pp 100154- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chitosan Microcarriers Collagen Gelatin IgG production Biochemistry QD415-436
spellingShingle	Chitosan Microcarriers Collagen Gelatin IgG production Biochemistry QD415-436 Tejal Pant Vidhi Murarka Ratnesh Jain Prajakta Dandekar Chitosan based microcarriers for cellular growth and biologics production
description	Microcarrier technology has emerged as one of the promising platforms for large scale production of anchorage dependent cells. Cells grown on these carriers have been utilized for applications ranging from tissue engineering to vaccine production. Porous chitosan microcarriers (CMC), having diameter in the range of 250 – 300 µm and having suitability for cell adhesion and proliferation, were prepared and evaluated in this study. CMC were cross-linked by ionic gelation method, using sodium tripolyphosphate, and freeze dried to obtain porous structures. Further, CMC were coated with an extracellular matrix (ECM) component, collagen, and its derivative, gelatin. Subsequently, the ability of coated and uncoated CMC to support the growth of CHO cells secreting human immunoglobulin G (IgG) was evaluated qualitatively and quantitatively by fluorescently labeling live cells and determining metabolically active cells respectively. IgG secretion from these cells was determined as a measure of cell functionality. After culturing cell laden CMC for 11 days, uncoated carriers were found to be more favorable for both, cell growth and productivity. The IgG productivity was 97.85, 74.26 and 84.12 ng/ mL for uncoated, collagen and gelatin coated CMC. CMC, based on its intrinsic properties and without the necessity of surface modifications, acted as a promising support matrix for culturing functionally active adherent cells, in suspension culture.
format	article
author	Tejal Pant Vidhi Murarka Ratnesh Jain Prajakta Dandekar
author_facet	Tejal Pant Vidhi Murarka Ratnesh Jain Prajakta Dandekar
author_sort	Tejal Pant
title	Chitosan based microcarriers for cellular growth and biologics production
title_short	Chitosan based microcarriers for cellular growth and biologics production
title_full	Chitosan based microcarriers for cellular growth and biologics production
title_fullStr	Chitosan based microcarriers for cellular growth and biologics production
title_full_unstemmed	Chitosan based microcarriers for cellular growth and biologics production
title_sort	chitosan based microcarriers for cellular growth and biologics production
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/d8ea62bf53964f96845bdd8bf3be902f
work_keys_str_mv	AT tejalpant chitosanbasedmicrocarriersforcellulargrowthandbiologicsproduction AT vidhimurarka chitosanbasedmicrocarriersforcellulargrowthandbiologicsproduction AT ratneshjain chitosanbasedmicrocarriersforcellulargrowthandbiologicsproduction AT prajaktadandekar chitosanbasedmicrocarriersforcellulargrowthandbiologicsproduction
_version_	1718445242713112576

Chitosan based microcarriers for cellular growth and biologics production

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