Overcoming biomass recalcitrance by combining genetically modified switchgrass and cellulose solvent-based lignocellulose pretreatment.

Decreasing lignin content of plant biomass by genetic engineering is believed to mitigate biomass recalcitrance and improve saccharification efficiency of plant biomass. In this study, we compared two different pretreatment methods (i.e., dilute acid and cellulose solvent) on transgenic plant biomas...

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Autores principales:	Noppadon Sathitsuksanoh, Bin Xu, Bingyu Zhao, Y-H Percival Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d8ecbd80466b4358a8bece10ce640486
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spelling	oai:doaj.org-article:d8ecbd80466b4358a8bece10ce6404862021-11-18T08:53:24ZOvercoming biomass recalcitrance by combining genetically modified switchgrass and cellulose solvent-based lignocellulose pretreatment.1932-620310.1371/journal.pone.0073523https://doaj.org/article/d8ecbd80466b4358a8bece10ce6404862013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24086283/?tool=EBIhttps://doaj.org/toc/1932-6203Decreasing lignin content of plant biomass by genetic engineering is believed to mitigate biomass recalcitrance and improve saccharification efficiency of plant biomass. In this study, we compared two different pretreatment methods (i.e., dilute acid and cellulose solvent) on transgenic plant biomass samples having different lignin contents and investigated biomass saccharification efficiency. Without pretreatment, no correlation was observed between lignin contents of plant biomass and saccharification efficiency. After dilute acid pretreatment, a strong negative correlation between lignin content of plant samples and overall glucose release was observed, wherein the highest overall enzymatic glucan digestibility was 70% for the low-lignin sample. After cellulose solvent- and organic solvent-based lignocellulose fractionation pretreatment, there was no strong correlation between lignin contents and high saccharification efficiencies obtained (i.e., 80-90%). These results suggest that the importance of decreasing lignin content in plant biomass to saccharification was largely dependent on pretreatment choice and conditions.Noppadon SathitsuksanohBin XuBingyu ZhaoY-H Percival ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e73523 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Noppadon Sathitsuksanoh Bin Xu Bingyu Zhao Y-H Percival Zhang Overcoming biomass recalcitrance by combining genetically modified switchgrass and cellulose solvent-based lignocellulose pretreatment.
description	Decreasing lignin content of plant biomass by genetic engineering is believed to mitigate biomass recalcitrance and improve saccharification efficiency of plant biomass. In this study, we compared two different pretreatment methods (i.e., dilute acid and cellulose solvent) on transgenic plant biomass samples having different lignin contents and investigated biomass saccharification efficiency. Without pretreatment, no correlation was observed between lignin contents of plant biomass and saccharification efficiency. After dilute acid pretreatment, a strong negative correlation between lignin content of plant samples and overall glucose release was observed, wherein the highest overall enzymatic glucan digestibility was 70% for the low-lignin sample. After cellulose solvent- and organic solvent-based lignocellulose fractionation pretreatment, there was no strong correlation between lignin contents and high saccharification efficiencies obtained (i.e., 80-90%). These results suggest that the importance of decreasing lignin content in plant biomass to saccharification was largely dependent on pretreatment choice and conditions.
format	article
author	Noppadon Sathitsuksanoh Bin Xu Bingyu Zhao Y-H Percival Zhang
author_facet	Noppadon Sathitsuksanoh Bin Xu Bingyu Zhao Y-H Percival Zhang
author_sort	Noppadon Sathitsuksanoh
title	Overcoming biomass recalcitrance by combining genetically modified switchgrass and cellulose solvent-based lignocellulose pretreatment.
title_short	Overcoming biomass recalcitrance by combining genetically modified switchgrass and cellulose solvent-based lignocellulose pretreatment.
title_full	Overcoming biomass recalcitrance by combining genetically modified switchgrass and cellulose solvent-based lignocellulose pretreatment.
title_fullStr	Overcoming biomass recalcitrance by combining genetically modified switchgrass and cellulose solvent-based lignocellulose pretreatment.
title_full_unstemmed	Overcoming biomass recalcitrance by combining genetically modified switchgrass and cellulose solvent-based lignocellulose pretreatment.
title_sort	overcoming biomass recalcitrance by combining genetically modified switchgrass and cellulose solvent-based lignocellulose pretreatment.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d8ecbd80466b4358a8bece10ce640486
work_keys_str_mv	AT noppadonsathitsuksanoh overcomingbiomassrecalcitrancebycombininggeneticallymodifiedswitchgrassandcellulosesolventbasedlignocellulosepretreatment AT binxu overcomingbiomassrecalcitrancebycombininggeneticallymodifiedswitchgrassandcellulosesolventbasedlignocellulosepretreatment AT bingyuzhao overcomingbiomassrecalcitrancebycombininggeneticallymodifiedswitchgrassandcellulosesolventbasedlignocellulosepretreatment AT yhpercivalzhang overcomingbiomassrecalcitrancebycombininggeneticallymodifiedswitchgrassandcellulosesolventbasedlignocellulosepretreatment
_version_	1718421232857120768

Overcoming biomass recalcitrance by combining genetically modified switchgrass and cellulose solvent-based lignocellulose pretreatment.

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