Tear cytokine response to multipurpose solutions for contact lenses

Carolyn M Kalsow,1 William T Reindel,2 Mohinder M Merchea,2 Kirk M Bateman,2,3 Joseph T Barr21Ocular Research Services, Mendon, NY, USA; 2Bausch and Lomb, Inc, Rochester, NY, USA; 3Statistics and Data Corporation, Tempe, AZ, USAPurpose: An increased risk of corneal infiltrative events has been noted...

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Autores principales:	Kalsow CM, Reindel WT, Merchea MM, Bateman KM, Barr JT
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/d8ee60986cb246238fecd06af73e5476
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spelling	oai:doaj.org-article:d8ee60986cb246238fecd06af73e54762021-12-02T02:45:54ZTear cytokine response to multipurpose solutions for contact lenses1177-54671177-5483https://doaj.org/article/d8ee60986cb246238fecd06af73e54762013-06-01T00:00:00Zhttp://www.dovepress.com/tear-cytokine-response-to-multipurpose-solutions-for-contact-lenses-a13495https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Carolyn M Kalsow,1 William T Reindel,2 Mohinder M Merchea,2 Kirk M Bateman,2,3 Joseph T Barr21Ocular Research Services, Mendon, NY, USA; 2Bausch and Lomb, Inc, Rochester, NY, USA; 3Statistics and Data Corporation, Tempe, AZ, USAPurpose: An increased risk of corneal infiltrative events has been noted with the use of certain contact lenses and multipurpose solutions (MPS). This study was designed to evaluate tear cytokine assay as a sensitive, objective, and quantitative measure of the ocular surface response to contact lens/MPS and to consider the assay's clinical relevance in the context of other measures of ocular surface response.Methods: Two MPS, ReNu® Fresh™ (RNF) and Opti-Free® RepleniSH (OFR), were used with daily wear silicone hydrogel contact lenses in a randomized, prospective crossover study involving 26 subjects. Clinical data collection (conjunctival hyperemia, ocular surface sensitivity, solution induced corneal staining (SICS) test score, and subjective responses) and tear cytokine assays were conducted masked. Responses were tracked as change from baseline throughout the experimental schedule.Results: Similar response patterns for several inflammatory cytokines were seen throughout both phases: subjects who received OFR in Phase I had mean tear concentrations that were generally higher than those of the RNF Phase I group. OFR Phase I subjects had significant (P < 0.01) increases over baseline at day 1 and/or following washout for 13 cytokines (cc chemokine ligands [CCL] 3, CCL5, CCL11, granulocyte-macrophage colony-stimulating factor [GM-CSF], interferon [INF]-γ, interleukin [IL]-2, IL-4, IL-5, IL-6, IL-13, IL-15, IL-17, tumor necrosis factor [TNF]-α). These changes were not observed in RNF Phase I subjects, even though SICS test scores increased. Phase I OFR subjects also had increased dryness, while RNF Phase I subjects had decreased bulbar hyperemia. No changes were detected with respect to limbal hyperemia or surface sensitivity thresholds.Conclusion: The tear cytokine assay can detect and differentiate contact lens/MPS induced increases in inflammatory cytokines. Changes in cytokine levels were consistent with measurement of hyperemia and dryness but not with SICS scores, thereby suggesting a proinflammatory response to OFR compared to RNF that is not related to SICS test score. Tear cytokine profiles may be useful for reconciling clinical relevance of test results and in revealing signaling involved in the development of corneal infiltrative events.Keywords: multiplex bead assay, corneal staining, immunoassay, corneal sensitivity, inflammationKalsow CMReindel WTMerchea MMBateman KMBarr JTDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2013, Iss default, Pp 1291-1302 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Kalsow CM Reindel WT Merchea MM Bateman KM Barr JT Tear cytokine response to multipurpose solutions for contact lenses
description	Carolyn M Kalsow,1 William T Reindel,2 Mohinder M Merchea,2 Kirk M Bateman,2,3 Joseph T Barr21Ocular Research Services, Mendon, NY, USA; 2Bausch and Lomb, Inc, Rochester, NY, USA; 3Statistics and Data Corporation, Tempe, AZ, USAPurpose: An increased risk of corneal infiltrative events has been noted with the use of certain contact lenses and multipurpose solutions (MPS). This study was designed to evaluate tear cytokine assay as a sensitive, objective, and quantitative measure of the ocular surface response to contact lens/MPS and to consider the assay's clinical relevance in the context of other measures of ocular surface response.Methods: Two MPS, ReNu® Fresh™ (RNF) and Opti-Free® RepleniSH (OFR), were used with daily wear silicone hydrogel contact lenses in a randomized, prospective crossover study involving 26 subjects. Clinical data collection (conjunctival hyperemia, ocular surface sensitivity, solution induced corneal staining (SICS) test score, and subjective responses) and tear cytokine assays were conducted masked. Responses were tracked as change from baseline throughout the experimental schedule.Results: Similar response patterns for several inflammatory cytokines were seen throughout both phases: subjects who received OFR in Phase I had mean tear concentrations that were generally higher than those of the RNF Phase I group. OFR Phase I subjects had significant (P < 0.01) increases over baseline at day 1 and/or following washout for 13 cytokines (cc chemokine ligands [CCL] 3, CCL5, CCL11, granulocyte-macrophage colony-stimulating factor [GM-CSF], interferon [INF]-γ, interleukin [IL]-2, IL-4, IL-5, IL-6, IL-13, IL-15, IL-17, tumor necrosis factor [TNF]-α). These changes were not observed in RNF Phase I subjects, even though SICS test scores increased. Phase I OFR subjects also had increased dryness, while RNF Phase I subjects had decreased bulbar hyperemia. No changes were detected with respect to limbal hyperemia or surface sensitivity thresholds.Conclusion: The tear cytokine assay can detect and differentiate contact lens/MPS induced increases in inflammatory cytokines. Changes in cytokine levels were consistent with measurement of hyperemia and dryness but not with SICS scores, thereby suggesting a proinflammatory response to OFR compared to RNF that is not related to SICS test score. Tear cytokine profiles may be useful for reconciling clinical relevance of test results and in revealing signaling involved in the development of corneal infiltrative events.Keywords: multiplex bead assay, corneal staining, immunoassay, corneal sensitivity, inflammation
format	article
author	Kalsow CM Reindel WT Merchea MM Bateman KM Barr JT
author_facet	Kalsow CM Reindel WT Merchea MM Bateman KM Barr JT
author_sort	Kalsow CM
title	Tear cytokine response to multipurpose solutions for contact lenses
title_short	Tear cytokine response to multipurpose solutions for contact lenses
title_full	Tear cytokine response to multipurpose solutions for contact lenses
title_fullStr	Tear cytokine response to multipurpose solutions for contact lenses
title_full_unstemmed	Tear cytokine response to multipurpose solutions for contact lenses
title_sort	tear cytokine response to multipurpose solutions for contact lenses
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/d8ee60986cb246238fecd06af73e5476
work_keys_str_mv	AT kalsowcm tearcytokineresponsetomultipurposesolutionsforcontactlenses AT reindelwt tearcytokineresponsetomultipurposesolutionsforcontactlenses AT mercheamm tearcytokineresponsetomultipurposesolutionsforcontactlenses AT batemankm tearcytokineresponsetomultipurposesolutionsforcontactlenses AT barrjt tearcytokineresponsetomultipurposesolutionsforcontactlenses
_version_	1718402191139536896

Tear cytokine response to multipurpose solutions for contact lenses

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