Tissue degeneration in ALS affected spinal cord evaluated by Raman spectroscopy

Abstract The Raman spectral features from spinal cord tissue sections of transgenic, ALS model mice and non-transgenic mice were compared using 457 nm excitation line, profiting from the favourable signal intensity obtained in the molecular fingerprint region at this wavelength. Transverse sections...

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Autores principales:	Gennaro Picardi, Alida Spalloni, Amanda Generosi, Barbara Paci, Nicola Biagio Mercuri, Marco Luce, Patrizia Longone, Antonio Cricenti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d8efe57bf9c04374911dafdc275187db
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spelling	oai:doaj.org-article:d8efe57bf9c04374911dafdc275187db2021-12-02T15:08:19ZTissue degeneration in ALS affected spinal cord evaluated by Raman spectroscopy10.1038/s41598-018-31469-42045-2322https://doaj.org/article/d8efe57bf9c04374911dafdc275187db2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-31469-4https://doaj.org/toc/2045-2322Abstract The Raman spectral features from spinal cord tissue sections of transgenic, ALS model mice and non-transgenic mice were compared using 457 nm excitation line, profiting from the favourable signal intensity obtained in the molecular fingerprint region at this wavelength. Transverse sections from four SOD1G93A mice at 75 days and from two at 90 days after birth were analysed and compared with sections of similarly aged control mice. The spectra acquired within the grey matter of tissue sections from the diseased mice is markedly different from the grey matter signature of healthy mice. In particular, we observe an intensity increase in the spectral windows 450–650 cm−1 and 1050–1200 cm−1, accompanied by an intensity decrease in the lipid contributions at ~1660 cm−1, ~1440 cm−1 and ~1300 cm−1. Axons demyelination, loss of lipid structural order and the proliferation and aggregation of branched proteoglycans are related to the observed spectral modifications. Furthermore, the grey and white matter components of the spinal cord sections could also be spectrally distinguished, based on the relative intensity of characteristic lipid and protein bands. Raman spectra acquired from the white matter regions of the SOD1G93A mice closely resembles those from control mice.Gennaro PicardiAlida SpalloniAmanda GenerosiBarbara PaciNicola Biagio MercuriMarco LucePatrizia LongoneAntonio CricentiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gennaro Picardi Alida Spalloni Amanda Generosi Barbara Paci Nicola Biagio Mercuri Marco Luce Patrizia Longone Antonio Cricenti Tissue degeneration in ALS affected spinal cord evaluated by Raman spectroscopy
description	Abstract The Raman spectral features from spinal cord tissue sections of transgenic, ALS model mice and non-transgenic mice were compared using 457 nm excitation line, profiting from the favourable signal intensity obtained in the molecular fingerprint region at this wavelength. Transverse sections from four SOD1G93A mice at 75 days and from two at 90 days after birth were analysed and compared with sections of similarly aged control mice. The spectra acquired within the grey matter of tissue sections from the diseased mice is markedly different from the grey matter signature of healthy mice. In particular, we observe an intensity increase in the spectral windows 450–650 cm−1 and 1050–1200 cm−1, accompanied by an intensity decrease in the lipid contributions at ~1660 cm−1, ~1440 cm−1 and ~1300 cm−1. Axons demyelination, loss of lipid structural order and the proliferation and aggregation of branched proteoglycans are related to the observed spectral modifications. Furthermore, the grey and white matter components of the spinal cord sections could also be spectrally distinguished, based on the relative intensity of characteristic lipid and protein bands. Raman spectra acquired from the white matter regions of the SOD1G93A mice closely resembles those from control mice.
format	article
author	Gennaro Picardi Alida Spalloni Amanda Generosi Barbara Paci Nicola Biagio Mercuri Marco Luce Patrizia Longone Antonio Cricenti
author_facet	Gennaro Picardi Alida Spalloni Amanda Generosi Barbara Paci Nicola Biagio Mercuri Marco Luce Patrizia Longone Antonio Cricenti
author_sort	Gennaro Picardi
title	Tissue degeneration in ALS affected spinal cord evaluated by Raman spectroscopy
title_short	Tissue degeneration in ALS affected spinal cord evaluated by Raman spectroscopy
title_full	Tissue degeneration in ALS affected spinal cord evaluated by Raman spectroscopy
title_fullStr	Tissue degeneration in ALS affected spinal cord evaluated by Raman spectroscopy
title_full_unstemmed	Tissue degeneration in ALS affected spinal cord evaluated by Raman spectroscopy
title_sort	tissue degeneration in als affected spinal cord evaluated by raman spectroscopy
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d8efe57bf9c04374911dafdc275187db
work_keys_str_mv	AT gennaropicardi tissuedegenerationinalsaffectedspinalcordevaluatedbyramanspectroscopy AT alidaspalloni tissuedegenerationinalsaffectedspinalcordevaluatedbyramanspectroscopy AT amandagenerosi tissuedegenerationinalsaffectedspinalcordevaluatedbyramanspectroscopy AT barbarapaci tissuedegenerationinalsaffectedspinalcordevaluatedbyramanspectroscopy AT nicolabiagiomercuri tissuedegenerationinalsaffectedspinalcordevaluatedbyramanspectroscopy AT marcoluce tissuedegenerationinalsaffectedspinalcordevaluatedbyramanspectroscopy AT patrizialongone tissuedegenerationinalsaffectedspinalcordevaluatedbyramanspectroscopy AT antoniocricenti tissuedegenerationinalsaffectedspinalcordevaluatedbyramanspectroscopy
_version_	1718388217033523200

Tissue degeneration in ALS affected spinal cord evaluated by Raman spectroscopy

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