Rickettsia species in African Anopheles mosquitoes.

<h4>Background</h4>There is higher rate of R. felis infection among febrile patients than in healthy people in Sub-Saharan Africa, predominantly in the rainy season. Mosquitoes possess a high vectorial capacity and, because of their abundance and aggressiveness, likely play a role in ric...

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Autores principales:	Cristina Socolovschi, Frédéric Pages, Mamadou O Ndiath, Pavel Ratmanov, Didier Raoult
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d8f09ede0535494c98fec02e41f39808
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spelling	oai:doaj.org-article:d8f09ede0535494c98fec02e41f398082021-11-18T08:10:37ZRickettsia species in African Anopheles mosquitoes.1932-620310.1371/journal.pone.0048254https://doaj.org/article/d8f09ede0535494c98fec02e41f398082012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23118963/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>There is higher rate of R. felis infection among febrile patients than in healthy people in Sub-Saharan Africa, predominantly in the rainy season. Mosquitoes possess a high vectorial capacity and, because of their abundance and aggressiveness, likely play a role in rickettsial epidemiology.<h4>Methodology/principal findings</h4>Quantitative and traditional PCR assays specific for Rickettsia genes detected rickettsial DNA in 13 of 848 (1.5%) Anopheles mosquitoes collected from Côte d'Ivoire, Gabon, and Senegal. R. felis was detected in one An. gambiae molecular form S mosquito collected from Kahin, Côte d'Ivoire (1/77, 1.3%). Additionally, a new Rickettsia genotype was detected in five An. gambiae molecular form S mosquitoes collected from Côte d'Ivoire (5/77, 6.5%) and one mosquito from Libreville, Gabon (1/88, 1.1%), as well as six An. melas (6/67, 9%) mosquitoes collected from Port Gentil, Gabon. A sequence analysis of the gltA, ompB, ompA and sca4 genes indicated that this new Rickettsia sp. is closely related to R. felis. No rickettsial DNA was detected from An. funestus, An. arabiensis, or An. gambiae molecular form M mosquitoes. Additionally, a BLAST analysis of the gltA sequence from the new Rickettsia sp. resulted in a 99.71% sequence similarity to a species (JQ674485) previously detected in a blood sample of a Senegalese patient with a fever from the Bandafassi village, Kedougou region.<h4>Conclusion</h4>R. felis was detected for the first time in An. gambiae molecular form S, which represents the major African malaria vector. The discovery of R. felis, as well as a new Rickettsia species, in mosquitoes raises new issues with respect to African rickettsial epidemiology that need to be investigated, such as bacterial isolation, the degree of the vectorial capacity of mosquitoes, the animal reservoirs, and human pathogenicity.Cristina SocolovschiFrédéric PagesMamadou O NdiathPavel RatmanovDidier RaoultPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e48254 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cristina Socolovschi Frédéric Pages Mamadou O Ndiath Pavel Ratmanov Didier Raoult Rickettsia species in African Anopheles mosquitoes.
description	<h4>Background</h4>There is higher rate of R. felis infection among febrile patients than in healthy people in Sub-Saharan Africa, predominantly in the rainy season. Mosquitoes possess a high vectorial capacity and, because of their abundance and aggressiveness, likely play a role in rickettsial epidemiology.<h4>Methodology/principal findings</h4>Quantitative and traditional PCR assays specific for Rickettsia genes detected rickettsial DNA in 13 of 848 (1.5%) Anopheles mosquitoes collected from Côte d'Ivoire, Gabon, and Senegal. R. felis was detected in one An. gambiae molecular form S mosquito collected from Kahin, Côte d'Ivoire (1/77, 1.3%). Additionally, a new Rickettsia genotype was detected in five An. gambiae molecular form S mosquitoes collected from Côte d'Ivoire (5/77, 6.5%) and one mosquito from Libreville, Gabon (1/88, 1.1%), as well as six An. melas (6/67, 9%) mosquitoes collected from Port Gentil, Gabon. A sequence analysis of the gltA, ompB, ompA and sca4 genes indicated that this new Rickettsia sp. is closely related to R. felis. No rickettsial DNA was detected from An. funestus, An. arabiensis, or An. gambiae molecular form M mosquitoes. Additionally, a BLAST analysis of the gltA sequence from the new Rickettsia sp. resulted in a 99.71% sequence similarity to a species (JQ674485) previously detected in a blood sample of a Senegalese patient with a fever from the Bandafassi village, Kedougou region.<h4>Conclusion</h4>R. felis was detected for the first time in An. gambiae molecular form S, which represents the major African malaria vector. The discovery of R. felis, as well as a new Rickettsia species, in mosquitoes raises new issues with respect to African rickettsial epidemiology that need to be investigated, such as bacterial isolation, the degree of the vectorial capacity of mosquitoes, the animal reservoirs, and human pathogenicity.
format	article
author	Cristina Socolovschi Frédéric Pages Mamadou O Ndiath Pavel Ratmanov Didier Raoult
author_facet	Cristina Socolovschi Frédéric Pages Mamadou O Ndiath Pavel Ratmanov Didier Raoult
author_sort	Cristina Socolovschi
title	Rickettsia species in African Anopheles mosquitoes.
title_short	Rickettsia species in African Anopheles mosquitoes.
title_full	Rickettsia species in African Anopheles mosquitoes.
title_fullStr	Rickettsia species in African Anopheles mosquitoes.
title_full_unstemmed	Rickettsia species in African Anopheles mosquitoes.
title_sort	rickettsia species in african anopheles mosquitoes.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d8f09ede0535494c98fec02e41f39808
work_keys_str_mv	AT cristinasocolovschi rickettsiaspeciesinafricananophelesmosquitoes AT fredericpages rickettsiaspeciesinafricananophelesmosquitoes AT mamadouondiath rickettsiaspeciesinafricananophelesmosquitoes AT pavelratmanov rickettsiaspeciesinafricananophelesmosquitoes AT didierraoult rickettsiaspeciesinafricananophelesmosquitoes
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Rickettsia species in African Anopheles mosquitoes.

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