Effect of X-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood

Abstract The broad clinical acceptance of intraoperative blood salvage and its applications in cancer surgery remain controversial. Until now, a method that can safely eliminate cancer cells while preserving erythrocytes does not exist. Here, we investigated whether X-ray generated from linear accel...

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Autores principales:	Feng-Jiang Zhang, Jin-Ting Yang, Li-Hui Tang, Wen-Na Wang, Kai Sun, Yue Ming, Kanhar Ghulam Muhammad, Yin-Fei Zheng, Min Yan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d8fc4bf9d08444038c999a5dc9de05b7
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spelling	oai:doaj.org-article:d8fc4bf9d08444038c999a5dc9de05b72021-12-02T12:32:45ZEffect of X-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood10.1038/s41598-017-08405-z2045-2322https://doaj.org/article/d8fc4bf9d08444038c999a5dc9de05b72017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08405-zhttps://doaj.org/toc/2045-2322Abstract The broad clinical acceptance of intraoperative blood salvage and its applications in cancer surgery remain controversial. Until now, a method that can safely eliminate cancer cells while preserving erythrocytes does not exist. Here, we investigated whether X-ray generated from linear accelerator irradiation at a certain dose can kill hepatocarcinoma cells while preserving erythrocytes. HepG2, SK-Hep1 or Huh7 cells were mixed into the aliquots of erythrocytes obtained from healthy volunteers. After the mixed cells were exposed to 30 Gy and 50 Gy X-rays irradiation, the viability, clonogenicity, DNA synthesis and tumorigenicity of the tumor cells were determined by the MTT assay, plate colony formation, 5-ethynyl-2′-deoxyuridine incorporation, and subcutaneous xenograft implantation into immunocompromised mice. The ATP, 2,3-DPG, free Hb, osmotic fragility, blood gas variables in erythrocytes and morphology of erythrocytes at 0 h, 12 h, 24 h, 48 h, 72 h after irradiation were analyzed. X-ray irradiation at 30 Gy effectively inhibited the viability, proliferation, and tumorigenicity of HepG2, SK-Hep1 and Huh7 cells without noticeably damaging the ability of oxygen-carrying, membrane integrity and morphology of erythrocytes. Theses results suggest that X-ray at 30 Gy irradiation might be safe to eliminate hepatocarcinoma cells while preserving erythrocytes in salvaged blood.Feng-Jiang ZhangJin-Ting YangLi-Hui TangWen-Na WangKai SunYue MingKanhar Ghulam MuhammadYin-Fei ZhengMin YanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Feng-Jiang Zhang Jin-Ting Yang Li-Hui Tang Wen-Na Wang Kai Sun Yue Ming Kanhar Ghulam Muhammad Yin-Fei Zheng Min Yan Effect of X-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood
description	Abstract The broad clinical acceptance of intraoperative blood salvage and its applications in cancer surgery remain controversial. Until now, a method that can safely eliminate cancer cells while preserving erythrocytes does not exist. Here, we investigated whether X-ray generated from linear accelerator irradiation at a certain dose can kill hepatocarcinoma cells while preserving erythrocytes. HepG2, SK-Hep1 or Huh7 cells were mixed into the aliquots of erythrocytes obtained from healthy volunteers. After the mixed cells were exposed to 30 Gy and 50 Gy X-rays irradiation, the viability, clonogenicity, DNA synthesis and tumorigenicity of the tumor cells were determined by the MTT assay, plate colony formation, 5-ethynyl-2′-deoxyuridine incorporation, and subcutaneous xenograft implantation into immunocompromised mice. The ATP, 2,3-DPG, free Hb, osmotic fragility, blood gas variables in erythrocytes and morphology of erythrocytes at 0 h, 12 h, 24 h, 48 h, 72 h after irradiation were analyzed. X-ray irradiation at 30 Gy effectively inhibited the viability, proliferation, and tumorigenicity of HepG2, SK-Hep1 and Huh7 cells without noticeably damaging the ability of oxygen-carrying, membrane integrity and morphology of erythrocytes. Theses results suggest that X-ray at 30 Gy irradiation might be safe to eliminate hepatocarcinoma cells while preserving erythrocytes in salvaged blood.
format	article
author	Feng-Jiang Zhang Jin-Ting Yang Li-Hui Tang Wen-Na Wang Kai Sun Yue Ming Kanhar Ghulam Muhammad Yin-Fei Zheng Min Yan
author_facet	Feng-Jiang Zhang Jin-Ting Yang Li-Hui Tang Wen-Na Wang Kai Sun Yue Ming Kanhar Ghulam Muhammad Yin-Fei Zheng Min Yan
author_sort	Feng-Jiang Zhang
title	Effect of X-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood
title_short	Effect of X-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood
title_full	Effect of X-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood
title_fullStr	Effect of X-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood
title_full_unstemmed	Effect of X-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood
title_sort	effect of x-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d8fc4bf9d08444038c999a5dc9de05b7
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Effect of X-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood

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