Characteristics of Two Crustins from <i>Alvinocaris longirostris</i> in Hydrothermal Vents

Crustins are widely distributed among different crustacean groups. They are characterized by a whey acidic protein (WAP) domain, and most examined Crustins show activity against Gram-positive bacteria. This study reports two Crustins, Al-crus 3 and Al-crus 7, from hydrothermal vent shrimp, <i>...

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Autores principales:	Lu-Lu Guo, Shao-Lu Wang, Fang-Chao Zhu, Feng Xue, Li-Sheng He
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	crustins antibacterial peptides hydrothermal vent anti-Gram-negative bacteria Al-crus 3 and Al-crus 7 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d8fe8ae182554bfd9a5279acaaf295fd
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spelling	oai:doaj.org-article:d8fe8ae182554bfd9a5279acaaf295fd2021-11-25T18:12:43ZCharacteristics of Two Crustins from <i>Alvinocaris longirostris</i> in Hydrothermal Vents10.3390/md191106001660-3397https://doaj.org/article/d8fe8ae182554bfd9a5279acaaf295fd2021-10-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/600https://doaj.org/toc/1660-3397Crustins are widely distributed among different crustacean groups. They are characterized by a whey acidic protein (WAP) domain, and most examined Crustins show activity against Gram-positive bacteria. This study reports two Crustins, Al-crus 3 and Al-crus 7, from hydrothermal vent shrimp, <i>Alvinocaris longirostris</i>. Al-crus 3 and Al-crus 7 belong to Crustin Type IIa, with a similarity of about 51% at amino acid level. Antibacterial assays showed that Al-crus 3 mainly displayed activity against Gram-positive bacteria with MIC<sub>50</sub> values of 10–25 μM. However, Al-crus 7 not only displayed activity against Gram-positive bacteria but also against Gram-negative bacteria Imipenem-resistant <i>Acinetobacter baumannii</i>, in a sensitive manner. Notably, in the effective antibacterial spectrum, Methicillin-sensitive <i>Staphylococcus aureus</i>, <i>Escherichia coli</i> (ESBLs) and Imipenem-resistant <i>A</i><i>. baumannii</i> were drug-resistant pathogens. Narrowing down the sequence to the WAP domain, Al-crusWAP 3 and Al-crusWAP 7 demonstrated antibacterial activities but were weak. Additionally, the effects on bacteria did not significantly change after they were maintained at room temperature for 48 h. This indicated that Al-crus 3 and Al-crus 7 were relatively stable and convenient for transportation. Altogether, this study reported two new Crustins with specific characteristics. In particular, Al-crus 7 inhibited Gram-negative imipenem-resistant <i>A</i><i>. baumannii</i>.Lu-Lu GuoShao-Lu WangFang-Chao ZhuFeng XueLi-Sheng HeMDPI AGarticlecrustinsantibacterial peptideshydrothermal ventanti-Gram-negative bacteriaAl-crus 3 and Al-crus 7Biology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 600, p 600 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	crustins antibacterial peptides hydrothermal vent anti-Gram-negative bacteria Al-crus 3 and Al-crus 7 Biology (General) QH301-705.5
spellingShingle	crustins antibacterial peptides hydrothermal vent anti-Gram-negative bacteria Al-crus 3 and Al-crus 7 Biology (General) QH301-705.5 Lu-Lu Guo Shao-Lu Wang Fang-Chao Zhu Feng Xue Li-Sheng He Characteristics of Two Crustins from <i>Alvinocaris longirostris</i> in Hydrothermal Vents
description	Crustins are widely distributed among different crustacean groups. They are characterized by a whey acidic protein (WAP) domain, and most examined Crustins show activity against Gram-positive bacteria. This study reports two Crustins, Al-crus 3 and Al-crus 7, from hydrothermal vent shrimp, <i>Alvinocaris longirostris</i>. Al-crus 3 and Al-crus 7 belong to Crustin Type IIa, with a similarity of about 51% at amino acid level. Antibacterial assays showed that Al-crus 3 mainly displayed activity against Gram-positive bacteria with MIC<sub>50</sub> values of 10–25 μM. However, Al-crus 7 not only displayed activity against Gram-positive bacteria but also against Gram-negative bacteria Imipenem-resistant <i>Acinetobacter baumannii</i>, in a sensitive manner. Notably, in the effective antibacterial spectrum, Methicillin-sensitive <i>Staphylococcus aureus</i>, <i>Escherichia coli</i> (ESBLs) and Imipenem-resistant <i>A</i><i>. baumannii</i> were drug-resistant pathogens. Narrowing down the sequence to the WAP domain, Al-crusWAP 3 and Al-crusWAP 7 demonstrated antibacterial activities but were weak. Additionally, the effects on bacteria did not significantly change after they were maintained at room temperature for 48 h. This indicated that Al-crus 3 and Al-crus 7 were relatively stable and convenient for transportation. Altogether, this study reported two new Crustins with specific characteristics. In particular, Al-crus 7 inhibited Gram-negative imipenem-resistant <i>A</i><i>. baumannii</i>.
format	article
author	Lu-Lu Guo Shao-Lu Wang Fang-Chao Zhu Feng Xue Li-Sheng He
author_facet	Lu-Lu Guo Shao-Lu Wang Fang-Chao Zhu Feng Xue Li-Sheng He
author_sort	Lu-Lu Guo
title	Characteristics of Two Crustins from <i>Alvinocaris longirostris</i> in Hydrothermal Vents
title_short	Characteristics of Two Crustins from <i>Alvinocaris longirostris</i> in Hydrothermal Vents
title_full	Characteristics of Two Crustins from <i>Alvinocaris longirostris</i> in Hydrothermal Vents
title_fullStr	Characteristics of Two Crustins from <i>Alvinocaris longirostris</i> in Hydrothermal Vents
title_full_unstemmed	Characteristics of Two Crustins from <i>Alvinocaris longirostris</i> in Hydrothermal Vents
title_sort	characteristics of two crustins from <i>alvinocaris longirostris</i> in hydrothermal vents
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d8fe8ae182554bfd9a5279acaaf295fd
work_keys_str_mv	AT luluguo characteristicsoftwocrustinsfromialvinocarislongirostrisiinhydrothermalvents AT shaoluwang characteristicsoftwocrustinsfromialvinocarislongirostrisiinhydrothermalvents AT fangchaozhu characteristicsoftwocrustinsfromialvinocarislongirostrisiinhydrothermalvents AT fengxue characteristicsoftwocrustinsfromialvinocarislongirostrisiinhydrothermalvents AT lishenghe characteristicsoftwocrustinsfromialvinocarislongirostrisiinhydrothermalvents
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Characteristics of Two Crustins from <i>Alvinocaris longirostris</i> in Hydrothermal Vents

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