HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women

Iodine is an essential component for fetal neurodevelopment and maternal thyroid function. Urine iodine is the most widely used indicator of iodine status. In this study, a novel validated ion-pair HPLC–UV method was developed to measure iodine concentration in clinical samples. A sodium thiosulfate...

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Autores principales:	Aniceta A. Mikulska, Dorota Filipowicz, Franciszek K. Główka, Ewelina Szczepanek-Parulska, Marek Ruchała, Michał Bartecki, Marta Karaźniewicz-Łada
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ion-pair HPLC–UV method validation in vivo application iodine supplementation pregnancy Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/d9025bc5e8134fccbdbfa4097c7f8c02
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spelling	oai:doaj.org-article:d9025bc5e8134fccbdbfa4097c7f8c022021-11-25T18:27:20ZHPLC Analysis of the Urinary Iodine Concentration in Pregnant Women10.3390/molecules262267971420-3049https://doaj.org/article/d9025bc5e8134fccbdbfa4097c7f8c022021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6797https://doaj.org/toc/1420-3049Iodine is an essential component for fetal neurodevelopment and maternal thyroid function. Urine iodine is the most widely used indicator of iodine status. In this study, a novel validated ion-pair HPLC–UV method was developed to measure iodine concentration in clinical samples. A sodium thiosulfate solution was added to the urine sample to convert the total free iodine to iodide. Chromatographic separation was achieved in a Pursuit XRs C8 column. The mobile phase consisted of acetonitrile and a water phase containing 18-crown-6-ether, octylamine and sodium dihydrogen phosphate. Validation parameters, such as accuracy, precision, limits of detection and quantification, linearity and stability, were determined. Urinary samples from pregnant women were used to complete the validation and confirm the method’s applicability. In the studied population of 93 pregnant women, the median UIC was lower in the group without iodine supplementation (117 µg/L, confidence interval (%CI): 95; 138) than in the supplement group (133 µg/L, %CI: 109; 157). In conclusion, the newly established ion-pair HPLC–UV method was adequately precise, accurate and fulfilled validation the criteria for analyzing compounds in biological fluids. The method is less complicated and expensive than other frequently used assays and permits the identification of the iodine-deficient subjects.Aniceta A. MikulskaDorota FilipowiczFranciszek K. GłówkaEwelina Szczepanek-ParulskaMarek RuchałaMichał BarteckiMarta Karaźniewicz-ŁadaMDPI AGarticleion-pair HPLC–UV methodvalidationin vivo applicationiodine supplementationpregnancyOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6797, p 6797 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ion-pair HPLC–UV method validation in vivo application iodine supplementation pregnancy Organic chemistry QD241-441
spellingShingle	ion-pair HPLC–UV method validation in vivo application iodine supplementation pregnancy Organic chemistry QD241-441 Aniceta A. Mikulska Dorota Filipowicz Franciszek K. Główka Ewelina Szczepanek-Parulska Marek Ruchała Michał Bartecki Marta Karaźniewicz-Łada HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women
description	Iodine is an essential component for fetal neurodevelopment and maternal thyroid function. Urine iodine is the most widely used indicator of iodine status. In this study, a novel validated ion-pair HPLC–UV method was developed to measure iodine concentration in clinical samples. A sodium thiosulfate solution was added to the urine sample to convert the total free iodine to iodide. Chromatographic separation was achieved in a Pursuit XRs C8 column. The mobile phase consisted of acetonitrile and a water phase containing 18-crown-6-ether, octylamine and sodium dihydrogen phosphate. Validation parameters, such as accuracy, precision, limits of detection and quantification, linearity and stability, were determined. Urinary samples from pregnant women were used to complete the validation and confirm the method’s applicability. In the studied population of 93 pregnant women, the median UIC was lower in the group without iodine supplementation (117 µg/L, confidence interval (%CI): 95; 138) than in the supplement group (133 µg/L, %CI: 109; 157). In conclusion, the newly established ion-pair HPLC–UV method was adequately precise, accurate and fulfilled validation the criteria for analyzing compounds in biological fluids. The method is less complicated and expensive than other frequently used assays and permits the identification of the iodine-deficient subjects.
format	article
author	Aniceta A. Mikulska Dorota Filipowicz Franciszek K. Główka Ewelina Szczepanek-Parulska Marek Ruchała Michał Bartecki Marta Karaźniewicz-Łada
author_facet	Aniceta A. Mikulska Dorota Filipowicz Franciszek K. Główka Ewelina Szczepanek-Parulska Marek Ruchała Michał Bartecki Marta Karaźniewicz-Łada
author_sort	Aniceta A. Mikulska
title	HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women
title_short	HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women
title_full	HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women
title_fullStr	HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women
title_full_unstemmed	HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women
title_sort	hplc analysis of the urinary iodine concentration in pregnant women
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d9025bc5e8134fccbdbfa4097c7f8c02
work_keys_str_mv	AT anicetaamikulska hplcanalysisoftheurinaryiodineconcentrationinpregnantwomen AT dorotafilipowicz hplcanalysisoftheurinaryiodineconcentrationinpregnantwomen AT franciszekkgłowka hplcanalysisoftheurinaryiodineconcentrationinpregnantwomen AT ewelinaszczepanekparulska hplcanalysisoftheurinaryiodineconcentrationinpregnantwomen AT marekruchała hplcanalysisoftheurinaryiodineconcentrationinpregnantwomen AT michałbartecki hplcanalysisoftheurinaryiodineconcentrationinpregnantwomen AT martakarazniewiczłada hplcanalysisoftheurinaryiodineconcentrationinpregnantwomen
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HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women

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