Variations in morphology, physiology, and multiple bioactive constituents of Lonicerae Japonicae Flos under salt stress

Abstract Lonicerae Japonicae Flos (LJF) is an important traditional Chinese medicine for the treatment of various ailments and plays a vital role in improving global human health. However, as unable to escape from adversity, the quality of sessile organisms is dramatically affected by salt stress. T...

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Autores principales:	Zhichen Cai, Xunhong Liu, Huan Chen, Rong Yang, Jiajia Chen, Lisi Zou, Chengcheng Wang, Jiali Chen, Mengxia Tan, Yuqi Mei, Lifang Wei
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d906eb58b12345c49746c65d2f5361c0
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spelling	oai:doaj.org-article:d906eb58b12345c49746c65d2f5361c02021-12-02T10:54:23ZVariations in morphology, physiology, and multiple bioactive constituents of Lonicerae Japonicae Flos under salt stress10.1038/s41598-021-83566-62045-2322https://doaj.org/article/d906eb58b12345c49746c65d2f5361c02021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83566-6https://doaj.org/toc/2045-2322Abstract Lonicerae Japonicae Flos (LJF) is an important traditional Chinese medicine for the treatment of various ailments and plays a vital role in improving global human health. However, as unable to escape from adversity, the quality of sessile organisms is dramatically affected by salt stress. To systematically explore the quality formation of LJF in morphology, physiology, and bioactive constituents' response to multiple levels of salt stress, UFLC-QTRAP-MS/MS and multivariate statistical analysis were performed. Lonicera japonica Thunb. was planted in pots and placed in the field, then harvested after 35 days under salt stress. Indexes of growth, photosynthetic pigments, osmolytes, lipid peroxidation, and antioxidant enzymes were identified to evaluate the salt tolerance in LJF under different salt stresses (0, 100, 200, and 300 mM NaCl). Then, the total accumulation and dynamic variation of 47 bioactive constituents were quantitated. Finally, Partial least squares discrimination analysis and gray relational analysis were performed to systematically cluster, distinguish, and evaluate the samples, respectively. The results showed that 100 mM NaCl induced growth, photosynthetic, antioxidant activities, osmolytes, lipid peroxidation, and multiple bioactive constituents in LJF, which possessed the best quality. Additionally, a positive correlation was found between the accumulation of phenolic acids with antioxidant enzyme activity under salt stress, further confirming that phenolic acids could reduce oxidative damage. This study provides insight into the quality formation and valuable information to improve the LJF medicinal value under salt stress.Zhichen CaiXunhong LiuHuan ChenRong YangJiajia ChenLisi ZouChengcheng WangJiali ChenMengxia TanYuqi MeiLifang WeiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhichen Cai Xunhong Liu Huan Chen Rong Yang Jiajia Chen Lisi Zou Chengcheng Wang Jiali Chen Mengxia Tan Yuqi Mei Lifang Wei Variations in morphology, physiology, and multiple bioactive constituents of Lonicerae Japonicae Flos under salt stress
description	Abstract Lonicerae Japonicae Flos (LJF) is an important traditional Chinese medicine for the treatment of various ailments and plays a vital role in improving global human health. However, as unable to escape from adversity, the quality of sessile organisms is dramatically affected by salt stress. To systematically explore the quality formation of LJF in morphology, physiology, and bioactive constituents' response to multiple levels of salt stress, UFLC-QTRAP-MS/MS and multivariate statistical analysis were performed. Lonicera japonica Thunb. was planted in pots and placed in the field, then harvested after 35 days under salt stress. Indexes of growth, photosynthetic pigments, osmolytes, lipid peroxidation, and antioxidant enzymes were identified to evaluate the salt tolerance in LJF under different salt stresses (0, 100, 200, and 300 mM NaCl). Then, the total accumulation and dynamic variation of 47 bioactive constituents were quantitated. Finally, Partial least squares discrimination analysis and gray relational analysis were performed to systematically cluster, distinguish, and evaluate the samples, respectively. The results showed that 100 mM NaCl induced growth, photosynthetic, antioxidant activities, osmolytes, lipid peroxidation, and multiple bioactive constituents in LJF, which possessed the best quality. Additionally, a positive correlation was found between the accumulation of phenolic acids with antioxidant enzyme activity under salt stress, further confirming that phenolic acids could reduce oxidative damage. This study provides insight into the quality formation and valuable information to improve the LJF medicinal value under salt stress.
format	article
author	Zhichen Cai Xunhong Liu Huan Chen Rong Yang Jiajia Chen Lisi Zou Chengcheng Wang Jiali Chen Mengxia Tan Yuqi Mei Lifang Wei
author_facet	Zhichen Cai Xunhong Liu Huan Chen Rong Yang Jiajia Chen Lisi Zou Chengcheng Wang Jiali Chen Mengxia Tan Yuqi Mei Lifang Wei
author_sort	Zhichen Cai
title	Variations in morphology, physiology, and multiple bioactive constituents of Lonicerae Japonicae Flos under salt stress
title_short	Variations in morphology, physiology, and multiple bioactive constituents of Lonicerae Japonicae Flos under salt stress
title_full	Variations in morphology, physiology, and multiple bioactive constituents of Lonicerae Japonicae Flos under salt stress
title_fullStr	Variations in morphology, physiology, and multiple bioactive constituents of Lonicerae Japonicae Flos under salt stress
title_full_unstemmed	Variations in morphology, physiology, and multiple bioactive constituents of Lonicerae Japonicae Flos under salt stress
title_sort	variations in morphology, physiology, and multiple bioactive constituents of lonicerae japonicae flos under salt stress
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d906eb58b12345c49746c65d2f5361c0
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Variations in morphology, physiology, and multiple bioactive constituents of Lonicerae Japonicae Flos under salt stress

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