Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe

Tao Zhang,1 Fang Tian,2 Lin Long,1 Jianbo Liu,3 Xiaochun Wu4 1Department of Clinical Laboratory, Zaozhuang Municipal Center for Disease Control and Prevention, Zaozhuang, China; 2Department of Clinical Laboratory, Zaozhuang Municipal Maternal and Child Health Hospital, Zaozhuang, China; 3College of...

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Autores principales:	Zhang T, Tian F, Long L, Liu JB, Wu XC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Gold nanorods Platinum nanozyme Enzyme-linked immunosorbent assay(ELISA) Virus diagnosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d90e1c163b6d4b4ba6707933ae08a63d
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spelling	oai:doaj.org-article:d90e1c163b6d4b4ba6707933ae08a63d2021-12-02T10:40:22ZDiagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe1178-2013https://doaj.org/article/d90e1c163b6d4b4ba6707933ae08a63d2018-08-01T00:00:00Zhttps://www.dovepress.com/diagnosis-of-rubella-virus-using-antigen-conjugated-aupt-nanorods-as-n-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Tao Zhang,1 Fang Tian,2 Lin Long,1 Jianbo Liu,3 Xiaochun Wu4 1Department of Clinical Laboratory, Zaozhuang Municipal Center for Disease Control and Prevention, Zaozhuang, China; 2Department of Clinical Laboratory, Zaozhuang Municipal Maternal and Child Health Hospital, Zaozhuang, China; 3College of Opto-electronic Engineering, Zaozhuang University, Zaozhuang, China; 4CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, Beijing, China Background: As nanotechnology advances rapidly, the nanozymes which could replace protein enzymes in bioanalytical assays bring a new opportunity to the development of simple and sensitive diagnostic tools. Purpose: Antibody-conjugated Au-Pt core/shell nanorods (Au@Pt NRs) have been used for nanozyme probes for diagnosis of rubella virus. Au@Pt NRs, prepared by Au nanorod-mediated growth, exhibit peroxidase-like activities and could serve as an inexpensive replacement for horseradish peroxidase (HRP) in conjugation of antigen. Method: Using a capture enzyme-linked immunosorbant assay for the determination of rubella virus. Results: Compared with antibody-conjugated HRP, the antigen-conjugated Au@Pt NRs are more stable and more robust, but less expensive. Based on the enhanced catalytic properties of this nanozyme probe, it was found that the antigen-conjugated Au@Pt NRs-based enzyme-linked immunosorbant assay exhibited good sensitivity. Conclusion: Our results indicate that these antigen-conjugated Au@Pt NRs represent a suitable nanozyme probe for future clinical applications under various conditions. Keywords: gold nanorods, nanozyme, antigen conjugation, rubella virus, diagnosisZhang TTian FLong LLiu JBWu XCDove Medical PressarticleGold nanorodsPlatinumnanozymeEnzyme-linked immunosorbent assay(ELISA)Virus diagnosisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 4795-4805 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gold nanorods Platinum nanozyme Enzyme-linked immunosorbent assay(ELISA) Virus diagnosis Medicine (General) R5-920
spellingShingle	Gold nanorods Platinum nanozyme Enzyme-linked immunosorbent assay(ELISA) Virus diagnosis Medicine (General) R5-920 Zhang T Tian F Long L Liu JB Wu XC Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe
description	Tao Zhang,1 Fang Tian,2 Lin Long,1 Jianbo Liu,3 Xiaochun Wu4 1Department of Clinical Laboratory, Zaozhuang Municipal Center for Disease Control and Prevention, Zaozhuang, China; 2Department of Clinical Laboratory, Zaozhuang Municipal Maternal and Child Health Hospital, Zaozhuang, China; 3College of Opto-electronic Engineering, Zaozhuang University, Zaozhuang, China; 4CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, Beijing, China Background: As nanotechnology advances rapidly, the nanozymes which could replace protein enzymes in bioanalytical assays bring a new opportunity to the development of simple and sensitive diagnostic tools. Purpose: Antibody-conjugated Au-Pt core/shell nanorods (Au@Pt NRs) have been used for nanozyme probes for diagnosis of rubella virus. Au@Pt NRs, prepared by Au nanorod-mediated growth, exhibit peroxidase-like activities and could serve as an inexpensive replacement for horseradish peroxidase (HRP) in conjugation of antigen. Method: Using a capture enzyme-linked immunosorbant assay for the determination of rubella virus. Results: Compared with antibody-conjugated HRP, the antigen-conjugated Au@Pt NRs are more stable and more robust, but less expensive. Based on the enhanced catalytic properties of this nanozyme probe, it was found that the antigen-conjugated Au@Pt NRs-based enzyme-linked immunosorbant assay exhibited good sensitivity. Conclusion: Our results indicate that these antigen-conjugated Au@Pt NRs represent a suitable nanozyme probe for future clinical applications under various conditions. Keywords: gold nanorods, nanozyme, antigen conjugation, rubella virus, diagnosis
format	article
author	Zhang T Tian F Long L Liu JB Wu XC
author_facet	Zhang T Tian F Long L Liu JB Wu XC
author_sort	Zhang T
title	Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe
title_short	Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe
title_full	Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe
title_fullStr	Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe
title_full_unstemmed	Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe
title_sort	diagnosis of rubella virus using antigen-conjugated au@pt nanorods as nanozyme probe
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/d90e1c163b6d4b4ba6707933ae08a63d
work_keys_str_mv	AT zhangt diagnosisofrubellavirususingantigenconjugatedauptnanorodsasnanozymeprobe AT tianf diagnosisofrubellavirususingantigenconjugatedauptnanorodsasnanozymeprobe AT longl diagnosisofrubellavirususingantigenconjugatedauptnanorodsasnanozymeprobe AT liujb diagnosisofrubellavirususingantigenconjugatedauptnanorodsasnanozymeprobe AT wuxc diagnosisofrubellavirususingantigenconjugatedauptnanorodsasnanozymeprobe
_version_	1718396915007094784

Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe

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